Differential alterations of antioxidant defenses as bioindicators of mercury and cadmium toxicity in alfalfa

Sobrino-Plata, Juan; Ortega-Villasante, Cristina; Flores-Cáceres, María Laura; Escobar, Carolina; Campo, Francisca F. del; Hernández, Luís E.

doi:10.1016/j.chemosphere.2009.08.007

Cited by 118 publications

(112 citation statements)

References 36 publications

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“…Accumulated evidence suggests that GR plays an important role in the detoxification of Cd-induced ROS, possibly via the ascorbate-glutathione cycle. Increased GR activity in the roots exposed to Cd was reported in plants, including Phaseolus vulgaris , potato (Stroinski et al 1999), radish (Vitoria et al 2001), soybean , sugarcane , Arabidopsis thaliana and alfalfa (Sobrino-Plata et al 2009). Moreover, GR activity was enhanced in shoots and roots of alfalfa plants exposed to Cd (Sobrino-Plata et al 2009).…”

Section: Heavy Metal Stressmentioning

confidence: 96%

Involvement of AsA/DHA and GSH/GSSG Ratios in Gene and Protein Expression and in the Activation of Defence Mechanisms Under Abiotic Stress Conditions

Fotopoulos

Ziogas

Tanou

et al. 2010

Ascorbate-Glutathione Pathway and Stress Tolerance in Plants

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In a persistently changing environment there are many adverse abiotic stress conditions such as cold, heat, drought, salinity, heavy metal toxicity and oxygen deprivation, which remarkably influence plant growth and crop production. Plant cells produce oxygen radicals and their derivatives, so-called reactive oxygen species (ROS) during various processes associated with abiotic stress. Moreover, the generation of ROS is the main means for higher plants to transmit cellular signalling information concerning the changing environmental conditions. Therefore, plants have evolved inducible redox state-based sensing mechanisms that are activated or amplified in response to adverse environmental conditions. Ascorbate and glutathione, the key cellular redox buffers, are used for both detoxification of ROS and transmission of redox signals. In recent years, it has become clear that abiotic stress conditions induce changes in the reduction/oxidation (redox) state of signalling molecules, which in turn modulate gene and protein expression to increase plant acclimation to abiotic stress. This important redox state-related branch of science has given several clues in understanding the adaptive plant responses to different stressful regimes. In this chapter, an overview of the literature is briefly presented in terms of the main function of ascorbate and glutathione in plant cells. Further more, we describe how important forms of abiotic stress regulate the expression of genes and proteins involved in the ascorbate and glutathione redox sensing system.

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Section: Heavy Metal Stressmentioning

confidence: 96%

Involvement of AsA/DHA and GSH/GSSG Ratios in Gene and Protein Expression and in the Activation of Defence Mechanisms Under Abiotic Stress Conditions

Fotopoulos

Ziogas

Tanou

et al. 2010

Ascorbate-Glutathione Pathway and Stress Tolerance in Plants

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“…Cd is not active redox but contributes indirectly to oxidative stress by affecting the cellular thiol redox balance (Stochs & Bagchi 1995). As Cd generates an oxidative stress, we have highlighted the disturbance of some enzymes involved directly or indirectly in the antioxidative cellular mechanisms (Smeets et al 2009;Sobrino-Plata et al 2009;Szollosi et al 2009;Zhang et al 2010). All analyzed GPOX, as well as ÁSHNP, were present in dry pea seeds which allowed the antioxidative machinery to be active as soon as the enzymes were reactivated by seed imbibitions (Figures 6 and 7).…”

mentioning

confidence: 86%

Role for plant peroxiredoxin in cadmium chelation

Smiri

Jelali

Ghoul

2013

Journal of Plant Interactions

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Studies on heavy metal underline the role of thiols in plants and attribute tolerance to metal binding. The thiol and peroxiredoxins (Prx) contents and guaiacol peroxidase (GPOX) activity were analyzed in the cotyledons and embryo of pea (Pisum sativum L.) germinating seeds exposed to toxic Cd concentration. The 2-cysteine peroxiredoxin (2Cys-Prx) level as well as the non-protein thiol (ÁSHNP) pool increased in both tissues treated with Cd compared to the control. An oxidized dimer of 2Cys-Prx was resolved in the presence of Cd ions. The obtained results suggest that Prx constitute a main key target in Cd toxicity. Despite of the decrease in GPOX activity due to the generation of an intracellular oxidative stress, a protective action via increasing Prx expression on thiols is possible to improve the redox status.

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“…Biothiol profiles were analyzed by HPLC (Sobrino-Plata et al, 2009). Extracts (100 mL) were injected into a Mediterranea SEA18 column (250 x 4.6 mm; Teknokroma, Sant Cugat del Vallés, Spain), using an Agilent Technologies 1200 series HPLC system (Santa Clara, CA).…”

Section: Quantification Of Biothiolsmentioning

confidence: 99%

Cytokinin determines thiol-mediated arsenic tolerance and accumulation in Arabidopsis thaliana

et al. 2016

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The presence of arsenic in soil and water is a constant threat to plant growth in many regions of the world. Phytohormones act in the integration of growth control and stress response, but their role in plant responses to arsenic remains to be elucidated. Here, we show that arsenate [As(V)], the most prevalent arsenic chemical species in nature, causes severe depletion of endogenous cytokinins (CKs) in the model plant Arabidopsis (Arabidopsis thaliana). We found that CK signaling mutants and transgenic plants with reduced endogenous CK levels showed an As(V)-tolerant phenotype. Our data indicate that in CK-depleted plants exposed to As(V), transcript levels of As(V)/phosphate-transporters were similar or even higher than in wild-type plants. In contrast, CK depletion provoked the coordinated activation of As(V) tolerance mechanisms, leading to the accumulation of thiol compounds such as phytochelatins and glutathione, which are essential for arsenic sequestration. Transgenic CK-deficient Arabidopsis and tobacco lines show a marked increase in arsenic accumulation. Our findings indicate that CK is an important regulatory factor in plant adaptation to arsenic stress.Since the inception of life, arsenic in the biosphere has been a constant challenge to the survival of life forms. Although all organisms on earth have developed strategies to cope with this extremely toxic metalloid (Rosen, 2002;Tripathi et al., 2007), arsenic currently poses a major worldwide environmental problem (Naujokas et al., 2013).Arsenate [As(V)] is the most abundant chemical form of arsenic. Due to its structural similarity to phosphate (Pi), it is easily incorporated into plants and other organisms through Pi transporters. Once taken up by the cell, As(V) is rapidly reduced to arsenite [As(III)] by As (V) reductases. As(III) is either extruded from the cytoplasm or is sequestered by phytochelatins (PCs) and other related thiol-containing compounds and is compartmentalized into vacuoles (Tripathi et al., 2007).In plants, when As(V) is perceived, Pi transporters are rapidly downregulated, and thiol compound accumulation increases concomitantly to cope with the metalloid. These two responses are key As(V) tolerance strategies for natural plant populations (Meharg and Macnair 1992;Bleeker et al., 2006). Accumulation of PC and other related thiol-containing compounds is widely used by plants for heavy metal detoxification. PCs are synthesized from glutathione (GSH) by the enzyme PHYTOCHELATIN SYNTHASE1 (PCS1). As(V) induces PCS1, together with two other genes involved in GSH biosynthesis: g-GLUTAMYLCYSTEINE SYNTHETASE and GLUTATHIONE SYNTHETASE (GSH2; Sung et al., 2009). In addition, As(III) activates GSH and PC accumulation; As(V) reductase activity is thus essential for arsenic tolerance (Bleeker et al., 2006). Arabidopsis thaliana ARSENATE REDUCTASE QTL1 (AtARQ1, At2g21045; also termed HAC1; Chao et al., 2014), an As(V) reductaseencoding gene, is also transcriptionally regulated by As(V) (Sánchez-Bermejo et al., 2014). Arsenic tolerance thus ...

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Differential alterations of antioxidant defenses as bioindicators of mercury and cadmium toxicity in alfalfa

Cited by 118 publications

References 36 publications

Involvement of AsA/DHA and GSH/GSSG Ratios in Gene and Protein Expression and in the Activation of Defence Mechanisms Under Abiotic Stress Conditions

Involvement of AsA/DHA and GSH/GSSG Ratios in Gene and Protein Expression and in the Activation of Defence Mechanisms Under Abiotic Stress Conditions

Role for plant peroxiredoxin in cadmium chelation

Cytokinin determines thiol-mediated arsenic tolerance and accumulation in Arabidopsis thaliana

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