Differential antioxidant enzyme and thiol responses of tolerant and non-tolerant clones of Chloris barbata to cadmium-stress

Baisakhi, Barnali; Patra, Jita; Panigrahy, Rabindra K.; Panda, Brahma B.

doi:10.1007/s11738-003-0017-0

Cited by 10 publications

(5 citation statements)

References 27 publications

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“…This is in accordance with the results obtained by Wu et al (2003) that Cd-stress induced a concentration and gene type dependent oxidative stress response and changes in barley leaf blades characterised by the alteration pattern of antioxidative enzymes superoxide dismutase (SOD) and peroxidase (POD). The increase of total polyphenols content as a response to Cd-stress is similar, as Baisakhi et al (2003) determined in the tolerant clone of Chloris barbata Sw. in protein-thiol level. As Chai et al (2003) found in the French bean (Phaseolus vulgaris), the expression of stress-related genes as PvSR2 can enhance the Cd tolerance, esp.…”

Section: Resultssupporting

confidence: 78%

Effect of cadmium on polyphenol content in young barley plants (Hordeum vulgare L.)

Dudjak¹,

Lachman²,

Miholová³

et al. 2004

PLANT SOIL ENVIRON

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The effect of abiotic stress caused by cadmium on the total polyphenols content in root, shoots and leaf blades of barley was investigated in model experiments. Total polyphenols content was determined spectrophotometrically with Folin Ciocalteau’s reagent, Cd content by atomic absorption spectrometry (AAS) in 28-day-old plants. Treatment of the barley plants with Cd (1.10<sup>–6</sup> mol/l) in a nutrition solution caused the increase in the total polyphenols in all parts of the plant. The highest increase was found in the leaf blades (35.2%, 8340 mg/kg d.m.), a lesser increase found in the shoots (16.7%, 3590 mg/kg d.m.), and the lowest increase found in the roots (10.3%, 3650 mg/kg d.m.). The highest Cd increase in the treated plants was found in the roots (10 400%, 240 mg/kg d.m.), the lesser in their shoots (4990%, 16.3 mg/kg d.m.), and the lowest in the leaf blades (2580%, 5.78 mg/kg d.m.). There was found a statistically significant increase (at least P < 0.05) in the total polyphenols and Cd contents in all investigated parts of the plant. Statistically significant differences (at least P < 0.05) of the total polyphenols and Cd contents among barley roots, shoots, and leaf blades were found.

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Section: Resultssupporting

confidence: 78%

Effect of cadmium on polyphenol content in young barley plants (Hordeum vulgare L.)

Dudjak¹,

Lachman²,

Miholová³

et al. 2004

PLANT SOIL ENVIRON

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“…Although the oxidative stress profiles were unaffected in Cdtolerant callus acclimatized to grown under high Cd concentration, these were significantly enhanced both in terms of lipid peroxidation and protein oxidation in callus exposed to Cd shock. The differential expression of lipid peroxidation between Cd-tolerant/resistant and -non-tolerant/ susceptible clones has also been reported in Chloros barbata (Baisakhi et al 2003) and in Salix viminalis (Landberg and Greger 2002). However, the present results could constitute initial evidence that Cd-acclimatized cells were more effective in coping with Cd-induced oxidative damage.…”

Section: Discussionsupporting

confidence: 70%

Glutathione-mediated Antioxidative Mechanisms in Sunflower (Helianthus Annuus L.) Cells in Response to Cadmium Stress

et al. 2005

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Cadmium (Cd) homeostasis and detoxification in sunflower (Helianthus annuus L.) cells differing in Cd sensitivity/tolerance were studied by analyzing the glutathione-mediated antioxidant mechanism vis-a`-vis phytochelatin biosynthesis in vitro. Calluses exposed to Cd-shock/-acclimatization (150 lM) were assayed for oxidative stress, reduced glutathione (GSH), glutathione disulfide (GSSG), phytochelatins (PCs) and reactive oxygen species (ROS). Although Cd did not induce any oxidative stress in Cd-tolerant callus (TCd), it generated oxidative stress in Cd-shock callus (SCd) both in terms of lipid peroxidation and protein oxidation. GSH/GSSG ratio remained similar to control values in the cadmium-acclimatized calluses. However, after acute treatment, there was a decline in both GSH and GSSG levels in SCd with concomitant reduction in the GSH/GSSG ratio. Analysis of PCs was performed using HPLC and mass spectrometry methods. PC concentration in TCd were approximately twice those that in SCd, showing in both cases a 1:2:1 relative proportion for PC n = 2 (PC2): PC n = 3 (PC3): PC n = 4 (PC4). Calluses growing in the presence of Cd developed an increased resistance to paraquat oxidative stress generation. These results indicated that PCs synthesis was an important mechanism for Cd detoxification in sunflower calluses, but the capacity to grow in the presence of Cd is related to the tissues ability to maintain high intracellular levels of GSH.

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“…Similarly, high increases in the level of TT and NPT were observed in maize plants treated with isoproturon, but thiols were elevated only during the first few days and by low doses of the tested compound [38]. In contrast, the content of NPT in non-tolerant clone of Chloris barbata L. increased under cadmium stress [39].…”

Section: Discussionmentioning

confidence: 99%

Aphid-Triggered Changes in Oxidative Damage Markers of Nucleic Acids, Proteins, and Lipids in Maize (Zea mays L.) Seedlings

Sytykiewicz

Łukasik

Goławska

et al. 2019

IJMS

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Prior experiments illustrated reactive oxygen species (ROS) overproduction in maize plants infested with bird-cherry-oat (Rhopalosiphum padi L.) aphids. However, there is no available data unveiling the impact of aphids feeding on oxidative damages of crucial macromolecules in maize tissues. Therefore, the purpose of the current study was to evaluate the scale of oxidative damages of genomic DNA, total RNA and mRNA, proteins, and lipids in seedling leaves of two maize genotypes (Złota Karłowa and Waza cvs—susceptible and relatively resistant to the aphids, respectively). The content of oxidized guanosine residues (8-hydroxy-2′-deoxyguanosine; 8-OHdG) in genomic DNA, 8-hydroxyguanosine (8-OHG) in RNA molecules, protein carbonyl groups, total thiols (T-SH), protein-bound thiols (PB-SH), non-protein thiols (NP-SH), malondialdehyde (MDA) and electrolyte leakage (EL) levels in maze plants were determined. In addition, the electrical penetration graphs (EPG) technique was used to monitor and the aphid stylet positioning and feeding modes in the hosts. Maize seedlings were infested with 0 (control), 30 or 60 R. padi adult apterae per plant. Substantial increases in the levels of RNA, protein and lipid oxidation markers in response to aphid herbivory, but no significant oxidative damages of genomic DNA, were found. Alterations in the studied parameters were dependent on maize genotype, insect abundance and infestation time.

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Differential antioxidant enzyme and thiol responses of tolerant and non-tolerant clones of Chloris barbata to cadmium-stress

Cited by 10 publications

References 27 publications

Effect of cadmium on polyphenol content in young barley plants (Hordeum vulgare L.)

Effect of cadmium on polyphenol content in young barley plants (Hordeum vulgare L.)

Glutathione-mediated Antioxidative Mechanisms in Sunflower (Helianthus Annuus L.) Cells in Response to Cadmium Stress

Aphid-Triggered Changes in Oxidative Damage Markers of Nucleic Acids, Proteins, and Lipids in Maize (Zea mays L.) Seedlings

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