Differential associations of Borrelia species with European badgers (Meles meles) and raccoon dogs (Nyctereutes procyonoides) in western Poland

Wodecka, Beata; Michalik, Jerzy; Lane, Robert S.; Nowak-Chmura, Magdalena; Wierzbicka, Anna

doi:10.1016/j.ttbdis.2016.05.008

Cited by 32 publications

(50 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11,17,18,22 Moreover, the rapid expansion of reservoir-adapted pathogens, such as Anaplasma, Bartonella, and Borrelia spp., has been described. 4,5,12,13,22,23,52 These previous studies and our present work suggest that raccoon dogs may serve as potential reservoirs for the transmission of VBPs. Furthermore, the increased population of wild animals may contribute to the transmission of VBPs between wildlife, humans, and companion animals in Korea.…”

Section: Discussionsupporting

confidence: 80%

See 1 more Smart Citation

Molecular Detection of Anaplasma, Bartonella, and Borrelia theileri in Raccoon Dogs (Nyctereutes procyonoides) in Korea

Kang

Chae

Cho

et al. 2018

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Anaplasmosis, cat-scratch disease, and Lyme disease are emerging vector-borne infectious diseases in Korea. Although the prevalence of vector-borne pathogens (VBPs) in domestic animals and vector arthropods has been documented, there is limited information on the presence of VBPs in wild animals. The raccoon dog (), a wild canid found in East Asia and Europe, represents a potential wildlife reservoir for zoonotic diseases. To investigate the prevalence of VBPs in raccoon dogs, 142 carcasses and 51 blood samples from captured raccoon dogs were collected from 2003 to 2010 and from 2008 to 2009, respectively, in Korea. In addition, 105 (14 larvae, 43 nymphs, 32 males, and 16 females) and nine (all female) were collected from three raccoon dogs. Samples of the spleen and blood were tested for the presence of VBPs by using nested polymerase chain reaction. Among the samples collected from 193 raccoon dogs and 114 ticks, two samples were positive for , four for, two for , and two for. To the best of our knowledge, this study is the largest survey of raccoon dogs aimed at the analysis of VBPs in this species. Moreover, the present study represents the first identification of ,, and in raccoon dogs in their native habitat (East Asia).

show abstract

Section: Discussionsupporting

confidence: 80%

“…2,3 Moreover, the prevalence of tick-borne pathogens that originate from raccoon dogs has been reported in Europe, Japan, and Korea. [4][5][6][7] Therefore, the increased population of raccoon dogs, and the resulting elevated zoonotic disease risk, represents a potential public health threat.…”

Section: Introductionmentioning

confidence: 99%

Molecular Detection of Anaplasma, Bartonella, and Borrelia theileri in Raccoon Dogs (Nyctereutes procyonoides) in Korea

Kang

Chae

Cho

et al. 2018

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…Recently, ticks resembling I. kaiseri were reported in Poland [33]. Although I. kaiseri was previously reported from Romania [34], this was not confirmed by later studies involving or reporting ticks of carnivores [8, 35–37].…”

Section: Discussionmentioning

confidence: 95%

Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females

et al. 2017

View full text Add to dashboard Cite

BackgroundIn Europe, hard ticks of the subgenus Pholeoixodes (Ixodidae: Ixodes) are usually associated with burrow-dwelling mammals and terrestrial birds. Reports of Pholeoixodes spp. from carnivores are frequently contradictory, and their identification is not based on key diagnostic characters. Therefore, the aims of the present study were to identify ticks collected from dogs, foxes and badgers in several European countries, and to reassess their systematic status with molecular analyses using two mitochondrial markers.ResultsBetween 2003 and 2017, 144 Pholeoixodes spp. ticks were collected in nine European countries. From accurate descriptions and comparison with type-materials, a simple illustrated identification key was compiled for adult females, by focusing on the shape of the anterior surface of basis capituli. Based on this key, 71 female ticks were identified as I. canisuga, 21 as I. kaiseri and 21 as I. hexagonus. DNA was extracted from these 113 female ticks, and from further 31 specimens. Fragments of two mitochondrial genes, cox1 (cytochrome c oxidase subunit 1) and 16S rRNA, were amplified and sequenced. Ixodes kaiseri had nine unique cox1 haplotypes, which showed 99.2–100% sequence identity, whereas I. canisuga and I. hexagonus had eleven and five cox1 haplotypes, respectively, with 99.5–100% sequence identity. The distribution of cox1 haplotypes reflected a geographical pattern. Pholeoixodes spp. ticks had fewer 16S rRNA haplotypes, with a lower degree of intraspecific divergence (99.5–100% sequence identity) and no geographical clustering. Phylogenetic analyses were in agreement with morphology: I. kaiseri and I. hexagonus (with the similar shape of the anterior surface of basis capituli) were genetically more closely related to each other than to I. canisuga. Phylogenetic analyses also showed that the subgenus Eschatocephalus (bat ticks) clustered within the subgenus Pholeoixodes.ConclusionsA simple, illustrated identification key is provided for female Pholeoixodes ticks of carnivores (including I. hexagonus and I. rugicollis) to prevent future misidentification of these species. It is also shown that I. kaiseri is more widespread in Europe than previously thought. Phylogenetic analyses suggest that the subgenus Pholeoixodes is not monophyletic: either the subgenus Eschatocephalus should be included in Pholeoixodes, or the latter subgenus should be divided, which is a task for future studies.

show abstract

“…Additionally, as reported in Additional file 1: Table S1, HRMA discrimination between C. l. familiaris and V. vulpes was straightforward thanks to their particular discriminant melting temperatures and to differences in the number of peaks (a single peak for dogs and two peaks for foxes). As for other wild European carnivores (badger Meles meles and raccoon dog Nyctereutes procyonoides [35]; marbled polecat Vormela peregusna and European mink Mustela lutreola [36]), the role of dogs and foxes in the epidemiology of TBDs is still not well-defined as they do not appear to be amplification hosts for TBEv [37], or reservoir hosts for Borrelia burgdorferi ( s.l .) [38] or A. phagocytophilum [28, 39], although all of these mammal species are known to carry these pathogens.…”

Section: Discussionmentioning

confidence: 99%

Identification of Ixodes ricinus blood meals using an automated protocol with high resolution melting analysis (HRMA) reveals the importance of domestic dogs as larval tick hosts in Italian alpine forests

et al. 2016

View full text Add to dashboard Cite

BackgroundIn Europe, Ixodes ricinus L. is the main vector of a variety of zoonotic pathogens, acquired through blood meals taken once per stage from a vertebrate host. Defining the main tick hosts in a given area is important for planning public health interventions; however, until recently, no robust molecular methods existed for blood meal identification from questing ticks. Here we improved the time- and cost-effectiveness of an HRMA protocol for blood meal analysis and used it to identify blood meal sources of sheep tick larvae from Italian alpine forests.MethodsNine hundred questing nymphs were collected using blanket-dragging in 18 extensive forests and 12 forest patches close to rural villages in the Province of Trento. Total DNA was either extracted manually, with the QIAamp DNA Investigator kit, or automatically using the KingFisher™ Flex Magnetic Particle Processors (KingFisher Cell and Tissue DNA Kit). Host DNA was amplified with six independent host group real-time PCR reactions and identified by means of HRMA. Statistical analyses were performed in R to assess the variables important for achieving successful identification and to compare host use in the two types of forest.ResultsAutomating DNA extraction improved time- and cost-effectiveness of the HRMA protocol, but identification success fell to 22.4% (KingFisher™) from 55.1% (QIAamp), with larval hosts identified in 215 of 848 questing nymphs; 23 mixed blood meals were noted. However, the list of hosts targeted by our primer sets was extended, improving the potential of the method. Host identification to species or genus level was possible for 137 and 102 blood meals, respectively. The most common hosts were Rodentia (28.9%) and, unexpectedly, Carnivora (28.4%), with domestic dogs accounting for 21.3% of all larval blood meals. Overall, Cetartiodactyla species fed 17.2% of larvae. Passeriformes (14.6%) fed a significantly higher proportion of larvae in forest patches (22.3%) than in extensive forest (9.6%), while Soricomorpha (10.9%) were more important hosts in extensive forest (15.2%) than in forest patches (4.3%).ConclusionsThe HRMA protocol for blood meal analysis is a valuable tool in the study of feeding ecology of sheep ticks, especially with the cost- and time- reductions introduced here. To our knowledge, we show for the first time that domestic dogs are important larval hosts in the Alps, which may have possible implications for tick-borne disease cycles in urbanized areas.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1901-y) contains supplementary material, which is available to authorized users.

show abstract

Differential associations of Borrelia species with European badgers (Meles meles) and raccoon dogs (Nyctereutes procyonoides) in western Poland

Cited by 32 publications

References 26 publications

Molecular Detection of Anaplasma, Bartonella, and Borrelia theileri in Raccoon Dogs (Nyctereutes procyonoides) in Korea

Molecular Detection of Anaplasma, Bartonella, and Borrelia theileri in Raccoon Dogs (Nyctereutes procyonoides) in Korea

Contributions to the phylogeny of Ixodes (Pholeoixodes) canisuga, I. (Ph.) kaiseri, I. (Ph.) hexagonus and a simple pictorial key for the identification of their females

Identification of Ixodes ricinus blood meals using an automated protocol with high resolution melting analysis (HRMA) reveals the importance of domestic dogs as larval tick hosts in Italian alpine forests

Contact Info

Product

Resources

About