Differential binding of Nocardia asteroides in the murine lung and brain suggests multiple ligands on the nocardial surface

Beaman, Blaine L.

doi:10.1128/iai.64.11.4859-4862.1996

Cited by 22 publications

(16 citation statements)

References 12 publications

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“…This experiment revealed the ability of the bacteria to induce behavioral disorders in the host animal while affecting neurological areas. Our results confirmed those obtained by Beaman, 1991 andBeaman &Tam , 2008. Our study revealed that N. cyriacigeorgica (not only the GUH-2 strain) and N. farcinica could induce dopaminergic neuron degeneration in C. elegans and mice, despite their origins. In the light of our results, N. farcinica 10152 seems to have had a greater neurotoxic effect on dopaminergic neurons than other tested strains.…”

Section: Resultssupporting

confidence: 91%

“…two clinical strains of N. cyriacigeorgica, one clinical strain of N. farcinica, and one environmental strain of N. asteroides (Table 1). The non-virulent status of N. asteroides 19247 defined by Beaman 1996, Beaman and Beaman 1998(Beaman 1996, Beaman & Beaman 1998) was confirmed in this study ( Table 3). The other strains induced behavioral disorders from day 6 post-infection (Table 3).…”

Section: Mouse Behavioral Disorders Induced By Nocardiasupporting

confidence: 80%

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Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral disorders

Claire

Zoropogui

Voisin

et al. 2017

Preprint

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Background. Parkinson’s disease (PD) is a disorder characterized by dopaminergic neuron programmed cell death. The etiology of PD remains uncertain—some cases are due to selected genes associated with familial heredity, others are due to environmental exposure to toxic components, but over 90% of cases have a sporadic origin. Nocardia are Actinobacteria that can cause human diseases like nocardiosis. This illness can lead to lung infection or central nervous system (CNS) invasion in both immunocompromised and immunocompetent individuals. The main species involved in CNS are N. farcinica, N. nova, N. brasiliensis and N. cyriacigeorgica. Some studies have highlighted the ability of N. cyriacigeorgica to induce Parkinson’s disease-like symptoms in animals. Actinobacteria are known to produce a large variety of secondary metabolites, some of which can be neurotoxic. We hypothesized that neurotoxic secondary metabolite production and the onset of PD-like symptoms in animals could be linked. Methods. Here we used a method to screen bacteria that could induce dopaminergic neurodegeneration before performing mouse experiments. Results.The nematode Caenorhabditis elegans allowed us to demonstrate that Nocardia strains belonging to N. cyriacigeorgica and N. farcinica species can induce dopaminergic neurodegeneration. Strains of interest involved with the nematodes in neurodegenerative disorders were then injected in mice. Infected mice had behavioral disorders that may be related to neuronal damage, thus confirming the ability of Nocardia strains to induce neurodegeneration. These behavioral disorders were induced by N. cyriacigeorgica species (N. cyriacigeorgica GUH-2 and N. cyriacigeorgica 44484) and N. farcinica 10152. Discussion.We conclude that C. elegans is a good model for detecting Nocardia strains involved in neurodegeneration. This model allowed us to detect bacteria with high neurodegenerative effects and which should be studied in mice to characterize the induced behavioral disorders and bacterial dissemination.

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Section: Resultssupporting

confidence: 91%

Section: Mouse Behavioral Disorders Induced By Nocardiasupporting

confidence: 80%

Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral disorders

Claire

Zoropogui

Voisin

et al. 2017

Preprint

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“…In the US it has been observed that nocardial infections are more frequent in the dry, windy climates of the southwest, possibly because such conditions facilitate aerosolization and dispersal of the organisms . Furthermore, there may be species and strain differences in virulence due to cell wall composition, inhibition of immune responses and a variety of other virulence factors . N. farcinica , for example, has been demonstrated to be more virulent than other Nocardia species in a mouse model .…”

Section: Epidemiology and Risk Factorsmentioning

confidence: 99%

Nocardia Infections in Solid Organ Transplantation

Clark

Reid

2013

American Journal of Transplantation

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“…Certain strains of N. asteroides, in log phase, actively invade through capillary endothelial cells in specific regions of the murine brain (4,10). In contrast, other strains of N. asteroides penetrate pulmonary epithelial cells but not endothelial cells in the brain (4).…”

mentioning

confidence: 99%

Filament Tip-Associated Antigens Involved in Adherence to and Invasion of Murine Pulmonary Epithelial Cells In Vivo and HeLa Cells In Vitro by Nocardia asteroides

Beaman¹,

Beaman

1998

Infect Immun

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The interactions of Nocardia asteroides GUH-2 with pulmonary epithelial cells of C57BL/6 mice and with HeLa cells were studied. Electron microscopy demonstrated that only the tips of log-phase cells penetrated pulmonary epithelial cells following intranasal administration, and nocardiae were recovered from the brain. Coccobacillary cells neither invaded nor disseminated. Serum from immunized mice (IMS) decreased attachment to and penetration of pulmonary epithelial cell surfaces by log-phase GUH-2 and inhibited spread to the brain. IMS was adsorbed against stationary-phase cells. Western immunoblots suggested that this adsorbed IMS was reactive primarily with 43- and 62-kDa proteins. Immunofluorescence showed that adsorbed IMS preferentially labeled the tips of log-phase GUH-2 cells. Since this IMS was reactive to culture filtrate antigens, several of these proteins were cut from gels, and mice were immunized. Sera against 62-, 55-, 43-, 36-, 31-, and 25-kDa antigens were obtained. The antisera against the 43- and 36-kDa proteins labeled the filament tips of GUH-2 cells. Only the antiserum against the 43-kDa antigen increased pulmonary clearance, inhibited apical attachment to and penetration of pulmonary epithelial cells, and prevented spread to the brain. An in vitro model with HeLa cells demonstrated that the tips of log-phase cells of GUH-2 adhered to and penetrated the surface of HeLa cells. Invasion assays with amikacin treatment demonstrated that nocardiae were internalized. Adsorbed IMS blocked attachment to and invasion of these cells. These data suggested that a filament tip-associated 43-kDa protein was involved in attachment to and invasion of pulmonary epithelial cells and HeLa cells by N. asteroides GUH-2.

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Differential binding of Nocardia asteroides in the murine lung and brain suggests multiple ligands on the nocardial surface

Cited by 22 publications

References 12 publications

Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral disorders

Virulence test using nematodes to prescreen Nocardia species capable of inducing neurodegeneration and behavioral disorders

Nocardia Infections in Solid Organ Transplantation

Filament Tip-Associated Antigens Involved in Adherence to and Invasion of Murine Pulmonary Epithelial Cells In Vivo and HeLa Cells In Vitro by Nocardia asteroides

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