Differential biofilm formation and chemical disinfection resistance of Escherichia coli on stainless steel and polystyrene tissue culture plate

Muazu, Anas; Rahman, Nor Iza A.; Aliyu, Sani; Abdullahi, Umar Faruk; Umar, Abubakar Abubakar; Ogidi, Jonathan Ajisafe

doi:10.18203/2320-6012.ijrms20151181

Cited by 7 publications

(11 citation statements)

References 25 publications

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“…This methodology involved shaking the cultures alongside extended periods of dehydration at 66% relative humidity (RH), interspersed with periods of hydration at 4, 7and 9 days. This was different to most studies on biofilms in the built environment that typically maintain conditions of high nutrient and moisture (Kostaki et al 2012;Muazu et al 2017;Gomes et al 2018).…”

Section: Introductionmentioning

confidence: 58%

Development of a high throughput and low cost model for the study of semi-dry biofilms

et al. 2020

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The persistence of microorganisms as biofilms on dry surfaces resistant to usual terminal cleaning methods may pose an additional risk of transmission of infections.In this study, the Centre for Disease Control (CDC) dry biofilm model (DBM) was adapted into a microtiter plate format (Model 1) and replicated to create a novel in vitro model that replicates conditions commonly encountered in the healthcare environment (Model 2). Biofilms of S. aureus grown in the two models were comparable to the biofilms of the CDC DBM in terms of recovered log 10 CFU/well. Assessment of antimicrobial tolerance of biofilms grown in the two models showedModel 2 a better model for biofilm formation. Confirmation of biofilms phenotype with an extracellular matrix deficient S. aureus suggested stress tolerance through a nonmatrix defined mechanism in organisms. This study highlights the importance of conditions maintained in bacterial growth as they affect biofilm phenotype and behaviour.

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Section: Introductionmentioning

confidence: 58%

Development of a high throughput and low cost model for the study of semi-dry biofilms

et al. 2020

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“…The microtiter plates washed twice or three times with 200 µl of phosphate buffered saline and allowed biofilms to fixed in the bottom of well after incubation for one hour. Crystal violet was used to stain biofilms, 200 µl of 1% crystal violet were add to each well and incubated at room temperature for 45 minutes followed by destained with 95% ethanol for 40 min at 37°C.The absorbance at 595 nm of each well was recorded by microtiter plate reader "Huma Reader-HS, Human GmbH, Wiesbaden, Germany" The percentage of anti-biofilm activity was calculated according to the following equation "[1−(A 595 of cells treated with AgNPs/A 595 of non-treated control cells)] × 100" (11).…”

Section: Sample Collection and Bacterial Identificationmentioning

confidence: 99%

Evaluation of bactericidal and anti-biofilm activities of silver nanoparticles against multidrug-resistant Gram-negative bacilli isolated from burn wound infections

Nasser¹

2018

Al-Kindy Col. Med. J

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Background: The emergence and spread of multidrug-resistant Gram-negative bacilliin burn wound infections related to biofilm formation, which lend to challenge in treatment with conventional antibiotics andprompting to search for novel antimicrobial agents to control the infections.Silver nanoparticles (AgNPs) have wide spectrum biological properties with different mechanisms of action and less toxicity towards human cells. Objective:The goal of this study was to evaluated the anti-bacterial and anti-biofilm activities of AgNPs alone and in combination with aminoglycoside (Amikacin) and β-lactam (Ampicillin) antibiotics against multidrug resistant Gram-negative bacilli (Pseudomonas aeruginosa, Escherichia coli, klebsiellapneumoniae) isolated from burn wound infections. Type of the study: Cross –sectional study. Methods: 70 clinical isolates of GNBtested for susceptibility tests by disk diffusion method against 10 antibiotics. The minimum inhibitory concentrations (MICs) of AgNPs and antibiotics were carried out according to the standard broth microdilution method, while synergistic interactions were evaluated by time kill-kinetic assays. Calgary method was applied for anti-biofilm activity. Results:Pseudomonas aeruginosa represented the majority of GNBisolated from burn wound infections 34 (48.5 %)followed by Klebsiella pneumonia 21 (30 %) and Escherichia coli 15 (21.5 %). Silver nanoparticles showed remarkable antibacterial activity against GNB that isolated from burn wound infections with the MICs between 25- 75 µg/ml. Aztreonam, amikacin and cefepime were the most effective antimicrobial drugagainst GNB isolates.Synergistic bactericidal effects were observed in two-drug combinations of AgNPswith broad-spectrum aminoglycoside (Amikacin) and β-lactam (Ampicillin) antibiotics against multidrug resistant GNB. In addition,AgNPsalone or in combination with ampicillin inhibited biofilm activity about 60 % – 75 % ofGNB,while combination of AgNPs withamikacin exhibited a powerful anti-biofilm activity and inhibition biofilm formation by 75% to 80%. Conclusion: The results confirmed a synergistic bactericidal effects and significant enhancing of anti-biofilm activity of AgNPs in combination with antibiotics (amikacin and ampicillin) against multidrug resistant GNB isolated from burn wound infections. These data suggest that AgNPs could beapplied as nanodrug for treatment of burn wound infections

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“…In summary, the proposed bacterial adherence assay (Figure 5.3), which is a composite of the processes and conditions in multiple literature procedures, [300][301][302][305][306][307][308] would begin with dilution to OD600 0.05 of an overnight (16 h) culture of E. coli NZRM 3647 in M63 minimal medium. As in Chapter 4 (Section 4.3), the coated, plastic test square (23 mm × 23 mm or 25 mm × 25 mm) would be prepared by applying a single layer of the test coating via the draw-down technique to both sides of a vinyl chloride/acetate copolymer, allowing each side to cure overnight, and cutting out a square to the desired dimensions.…”

Section: Methods Development With E Coli Nzrm 3647mentioning

confidence: 99%

“…291,[293][294][295]304 Regarding the length of incubation in the diluted culture of E. coli, multiple studies required incubation of the substrate for 24 h before rinsing it and detaching the bacteria. 301,302,305 Detachment was achieved by vortexing 1-2 min and/or sonication, 300,[305][306][307] and agar plating, specifically by the drop plate method, was performed to determine the colony forming units (CFU) per cm 2 of the substrate. 300,305 For the drop plate method (Figure 5.2), aliquots (10 µL) of each dilution were dispensed in small, separate drops on agar plates and then were allowed to absorb into the agar, followed by incubation of the plate and colony-counting within the drops.…”

Section: Bacterial Adherence To Coatings 51 Backgroundmentioning

confidence: 99%

“…301,302,305 Detachment was achieved by vortexing 1-2 min and/or sonication, 300,[305][306][307] and agar plating, specifically by the drop plate method, was performed to determine the colony forming units (CFU) per cm 2 of the substrate. 300,305 For the drop plate method (Figure 5.2), aliquots (10 µL) of each dilution were dispensed in small, separate drops on agar plates and then were allowed to absorb into the agar, followed by incubation of the plate and colony-counting within the drops. The countable dilution yielded 3-30 colonies per 10 µL drop.…”

Section: Bacterial Adherence To Coatings 51 Backgroundmentioning

confidence: 99%

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Selective Metal Coordination in Antifouling Coatings

Robinson¹

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<p>Marine biofouling is the accumulation of biological material (e.g. microorganisms, soft- and hard-fouling organisms) on the surface of an object submerged in seawater, and it remains a worldwide problem for shipping industries. The fouling of ship hulls results in a reduction of speed and manoeuvrability due to frictional drag, as well as increased fuel consumption and accelerated corrosion, and the exorbitant expenses and losses of efficiency attributed to biofouling have prompted the development of antifouling coatings. Current antifouling paints use copper as a biocidal agent, but copper-based paints are increasingly being banned due to environmental concerns about the non-target effects of leached copper. This project aims to circumvent these concerns and tightening regulations via a revolutionary concept: the development of marine antifouling paints that incorporate Cu(II)-selective ligands to draw the biocidal ingredient (i.e. Cu(II)) from seawater. A multistage strategy emerged for the development of this technology. First, criteria were established for the project’s ideal ligand, and ligands were synthesised or selected based on these criteria. Second, the ligands were incorporated in coatings through covalent modification of the paint binder or additives. Third, methodology was developed and implemented to test each coating’s ability to coordinate and retain Cu(II), as well as its subsequent ability to prevent microfouling by marine bacteria. The suitability of two ligand classes was assessed: acylhydrazones and tetraaza macrocycles, specifically cyclen. Unlike the acylhydrazones, cyclen met the established criteria and was initially evaluated as a curing agent and/or surface-modifier in a two-pack epoxy system with resin Epikote™ 235. However, the Cu(II)-loading by these coatings was relatively low, being at most ~0.05% w/w, and the modification of silica, a common paint additive, with cyclen was explored as an alternative formulation route. The method for the functionalisation of silica with cyclen was optimised, and the maximum Cu(II)-loading achieved by the product was 2.60% w/w. The cyclen-functionalised silica was incorporated on the surface of an epoxy coating, and a bacterial adherence assay was developed to assess the cellular attachment of marine bacterium Vibrio harveyi to this coating, which was found to be undeterred. Yet, the development of the strategy and testing methodology by which the project’s goals may be achieved provides a solid foundation for future work.</p>

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Differential biofilm formation and chemical disinfection resistance of Escherichia coli on stainless steel and polystyrene tissue culture plate

Cited by 7 publications

References 25 publications

Development of a high throughput and low cost model for the study of semi-dry biofilms

Development of a high throughput and low cost model for the study of semi-dry biofilms

Evaluation of bactericidal and anti-biofilm activities of silver nanoparticles against multidrug-resistant Gram-negative bacilli isolated from burn wound infections

Selective Metal Coordination in Antifouling Coatings

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