Differential capacity of kaolinite and birnessite to protect surface associated proteins against thermal degradation

Chacon, Stephany S.; García-Jaramillo, Manuel; Liu, Suet Yi; Ahmed, Musahid; Kleber, Markus

doi:10.1016/j.soilbio.2018.01.020

Cited by 7 publications

(12 citation statements)

References 53 publications

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“…6). These minerals tend to catalyze moderate changes to the structure and electronic state of adsorbed molecules, primarily through direct bonding, hydrolytic breakdown from reactions with surface OH, and heterogeneous oxidation with adsorbed O 2 123,126,127 .…”

Section: [H1] Catalysismentioning

confidence: 99%

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Dynamic interactions at the mineral–organic matter interface

Kleber

Bourg

Coward

et al. 2021

Nat Rev Earth Environ

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513

369

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Minerals are widely assumed to protect organic matter (OM) from degradation in the environment, promoting the persistence of carbon in soil and sediments. In this Review, we describe the mechanisms and processes operating at the mineral-organic interface as they relate to OM transformation dynamics. A broad set of interactions occur, with minerals adsorbing organic compounds to their surfaces and/or acting as catalysts for organic reactions. Minerals can serve as redox partners for OM through direct electron transfer or by generating reactive oxygen species, which then oxidize OM. Finally, the compartmentalization of soil and sediment by minerals creates unique microsites that host diverse microbial communities. Acknowledgement of this multiplicity of interactions suggests the general assumption that the mineral matrix provides a protective function for organic matter is overly simplistic. Future work must recognize adsorption as a condition for further reactions instead of as a final destination for organic adsorbates, and should consider the spatial and functional complexity that is characteristic of the environments where mineral-OM interactions are observed.

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Section: [H1] Catalysismentioning

confidence: 99%

“…6). Reduced clays (those with substituted transition metals), Mn oxides, sulfides and magnetite belong to this category 123,[127][128][129] . Mineral carbonates and sulfides, through major changes of surface acidity at the interface, play an important role in the hydrolytic breakdown of macromolecular organic molecules.…”

Section: [H1] Catalysismentioning

confidence: 99%

Dynamic interactions at the mineral–organic matter interface

Kleber

Bourg

Coward

et al. 2021

Nat Rev Earth Environ

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513

369

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“…

In soils and sediments, extracellular enzymes are in contact with mineral surfaces. Minerals may (a) serve as inert sorbent surfaces (Gianfreda et al, 2011); (b) act as reactants in chemical reactions (Chacon et al, 2018); and (c) serve as catalysts (Chacon et al, 2018;Reardon et al, 2016), thus inducing alterations to the adsorbed substrate. Of particular interest are modifications in enzyme activity that may be a direct consequence of adsorption to mineral surfaces (Allison, 2006;Quiquampoix & Burns, 2007;Zimmerman & Ahn, 2010).

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mentioning

confidence: 99%

The Important Role of Enzyme Adsorbing Capacity of Soil Minerals in Regulating β‐Glucosidase Activity

Sheng

Dong

Coffin

et al. 2022

Geophysical Research Letters

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Mineral surfaces are known for their ability to adsorb enzymes, but their effects on enzyme activity are not well studied. This study explores the extent to which interaction with primary silicates and secondary minerals affects the activity and longevity of a model soil enzyme β‐glucosidase (BG). The BG activity was correlated with the capacity‐related properties of minerals (controlling space for adsorption), including specific surface area (SSA) and the SSA‐normalized amount of BG adsorption, but not with the functional properties (controlling the mechanisms of adsorption) such as surface charge. BG conformational change is a function of SSA‐normalized amount of adsorption, suggesting that spatially more distributed enzyme molecules would have a higher probability of encounter with the substrate than the congested molecules. The highest decay rate of BG activity was observed in the presence of Mn‐oxide. Our results shed new light on the effect of mineral surface on enzyme activity.

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“…Previous studies − indicated that the propensity of proteins to become cleaved increased with decreasing pH, suggesting the process might be particularly efficient in, and possibly restricted to, acidic soils and environments. When we cumulated the number of unique peptide fragments over all four time points (Figure ), the previously noted pH dependence of protein fragmentation by birnessite disappeared.…”

Section: Resultsmentioning

confidence: 99%

“…The persistence of a protein involved in a mineral association, and, by extension, its functional lifespan, are thought to be constrained by eventual microbial degradation. However, previous research − demonstrated abiotic fragmentation of protein by manganese oxides, but little is known about the propensity of other relevant surface archetypes (such as negatively charged, positively charged, and predominantly neutral surfaces) to fragment a protein.…”

Section: Introductionmentioning

confidence: 99%

Mineral Surfaces as Agents of Environmental Proteolysis: Mechanisms and Controls

Chacon

Reardon

Burgess

et al. 2019

Environ. Sci. Technol.

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We investigated the extent to which contact with mineral surfaces affected the molecular integrity of a model protein, with an emphasis on identifying the mechanisms (hydrolysis, oxidation) and conditions leading to protein alteration. To this end, we studied the ability of four mineral surface archetypes (negatively charged, positively charged, neutral, redox-active) to abiotically fragment a well-characterized protein (GB1) as a function of pH and contact time. GB1 was exposed to the soil minerals montmorillonite, goethite, kaolinite, and birnessite at pH 5 and pH 7 for 1, 8, 24, and 168 hours and the supernatant was screened for peptide fragments using Tandem Mass Spectrometry. To distinguish between products of oxidative and hydrolytic cleavage, we combined results from the SEQUEST algorithm, which identifies protein fragments that were cleaved hydrolytically, with the output of a deconvolution algorithm (DECON-Routine) designed to identify oxidation fragments. All four minerals were able to induce protein cleavage. Manganese oxide was effective at both hydrolytic and oxidative cleavage. The fact that phyllosilicates-which are not redox active-induced oxidative cleavage indicates that surfaces acted as catalysts and not as reactants. Our results extend previous observations of proteolytic capabilities in soil minerals to the groups of phyllosilicates and Fe-oxides. We identified structural regions of the protein with particularly high susceptibility to cleavage (loops and beta strands) as well as regions that were entirely unaffected (alpha helix).

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Differential capacity of kaolinite and birnessite to protect surface associated proteins against thermal degradation

Cited by 7 publications

References 53 publications

Dynamic interactions at the mineral–organic matter interface

Dynamic interactions at the mineral–organic matter interface

The Important Role of Enzyme Adsorbing Capacity of Soil Minerals in Regulating β‐Glucosidase Activity

Mineral Surfaces as Agents of Environmental Proteolysis: Mechanisms and Controls

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