Differential composition of proteomes in sow colostrum and milk from anterior and posterior mammary glands1

Abstract

Piglets obtaining milk from anterior and middle mammary glands (MG) grow faster than those suckling posterior MG, but the underlying mechanisms are not clear. The purpose of this study was to investigate the differential proteomes of colostrum and milk secreted by anterior and posterior MG. Six healthy primiparous sows with 7 pairs of MG were used; the first and the second pairs were defined as anterior MG and the sixth and seventh pairs as posterior MG. Colostrum and milk were collected at d 1 and 14 after parturition, respectively. Comparative proteomics analysis was performed to identify the differentially expressed proteins in colostrum and milk secreted by anterior and posterior MG. Results show that protein composition in colostrum and milk varied markedly with the anatomical location of MG. Immunoglobulins, lactadherin, and haptoglobin were upregulated (P < 0.05) in colostrum from anterior MG compared with posterior MG. Concentrations of immunoglobulins and lactoferrin in milk from anterior MG were greater (P < 0.05) than milk from posterior MG. Moreover, concentration of proteins from somatic cells was greater (P < 0.05) in milk from posterior MG compared with anterior MG. Most proteins, in which abundance was upregulated in colostrum and milk from anterior MG, contribute to passive immunity, intestinal development of suckling piglets and epithelial integrity, and the health of MG. Collectively, these results indicate that in comparison with posterior MG, anterior MG are more active in protein synthesis and produce more immunoglobulins and lactoferrin in colostrum and milk.

Within-litter variation in birth weight: impact of nutritional status in the sow

“…Offspring that obtain milk from anterior and middle mammary glands (MGs) grow faster than those suckling posterior MG (Puppe and Tuchscherer, 1999;Kim et al, 2000). Besides the greater amount of colostrum and milk from anterior and middle MGs (Gill and Thomson, 1956), we reported that components in colostrum and milk secreted by anterior and posterior MGs are also distinctive (Wu W.Z. et al, 2010) in having proteins that are beneficial to passive immunity, intestinal development, and epithelial integrity; they include immunoglobulins and haptoglobin in colostrum and lactoferrin in milk from anterior MG, which are more abundant in anterior and middle MGs compared with posterior MG.…”

Análise Etológica Relacionada Ao Procedimento De Desgastar Ou Não Dentes De Lactentes Suínos

“…Após o nascimento, os leitões são direcionados a mamar o colostro, rico em imunoglobulinas e importante para sua imunidade passiva e desenvolvimento intestinal (23) . Durante este período, os leitões são expostos ao aparelho mamário da porca e, nas primeiras dezoito horas, elegem um teto preferencial, sendo que neste período ocorrem disputas entre indivíduos e cada lactente poderá mamar em três ou quatro tetos diferentes (24,25) .…”

Physiologie de l’immunité colostrale chez les porcins et facteurs de variation du rendement et de la qualité immunitaire du colostrum de la truie

“…Par contre, l'effet de la parité sur la concentration colostrale d'Igs est remarquable chez la truie avec une plus forte concentration d'IgG et d'IgA dans le colostrum des multipares (à partir de parité 4) (Quesnel, 2011b ;CarneyHinkle et al, 2013). L'effet de la position des tétines sur la qualité du colostrum a été aussi observé, le colostrum des mamelles antérieures étant plus riche en Igs que celui des postérieures (Wu et al, 2010).…”