Differential contractile actions of reactive oxygen species on rat aorta: Selective activation of ATP receptor by H2O2

Shen, Jianzhong; Zheng, Xiufeng; Kwan, Chiu‐Yin

doi:10.1016/s0024-3205(00)00539-7

Cited by 30 publications

(19 citation statements)

References 19 publications

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“…superoxide, hydrogen peroxide and hydroxyl radicals, are involved in sustained contraction. In support of this, Shen et al reported that pyrogallol (a superoxide donor), hydrogen peroxide, or vitamin C/Fe 2ϩ (a hydroxyl radical-generating system) contracted resting aortic rings from rats, 15) indicating that each ROS contributed to contraction. Zhang et al recently reported sustained contraction caused by the XϩXOD system used as superoxide donor in isolated porcine coronary artery rings, but only when endothelium was present.…”

Section: Fig 5 Inhibitory Effects Of Various Ros Scavengers On Dysfmentioning

confidence: 90%

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Sustained Contraction and Endothelial Dysfunction Induced by Reactive Oxygen Species in Porcine Coronary Artery

Ishihara

Sekine

Hatano

et al. 2008

Biological & Pharmaceutical Bulletin

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Reactive oxygen species (ROS), including superoxide anions, hydrogen peroxide and hydroxyl radicals, can be produced in vivo by mechanisms related to mitochondrial respiration, xanthine oxidase (XOD), nitric oxide synthase (NOS), NADPH oxidase, and arachidonic acid metabolism.1,2) Disruption to one or more of these mechanisms is thought to be responsible for the pathogenesis of various diseases. [3][4][5][6] Of these mechanisms, XOD may be involved in the generation of ROS in the endothelium during ischemia and/or reperfusion. 7,8) Roy and McCord demonstrated that xanthine dehydrogenase is proteolytically converted to XOD in tissues within seconds or minutes of an ischemic episode, depending on the tissue involved. 9) Although both enzymes are involved in the catabolism of purine compounds, the latter transfers electrons to molecular oxygen to yield the ROS, superoxide anions and hydrogen peroxide. 10,11) Thus, the deleterious effects of reoxygenation or reperfusion in ischemic tissues can therefore be explained on the basis of the ROS produced by the xanthine-XOD (XϩXOD) system. For a better understanding of the pathogenesis of tissue damage by ROS, it is important to clarify which species of oxygen intermediates are involved in these events, and whether or not there are differences in sensitivity to ROS among various tissues.The direct effects of ROS on cultured vascular endothelial cells or isolated, perfused tissues have been extensively investigated, either by using ROS directly, or using a ROS-generating system, such as the XϩXOD system. [12][13][14][15][16][17] In these studies, ROS scavengers are used to clarify the species of ROS involved in these effects. Among the various biological effects attributed to ROS, vascular endothelial dysfunction has consistently been noted, irrespective of the preparations used. 2,18) In addition, ROS generated by the XϩXOD system caused contraction of rat pulmonary artery 19) and canine basilar artery, 20) whereas it relaxed canine coronary artery, 21) implying the existence of species or tissue differences in response to ROS. Furthermore, the responses of arteries to ROS are well-known to be influenced by the presence of endothelium. 12,13,16) Thus, the responses of the vasculature, including the endothelium, to ROS are complex, and the sensitivity to ROS probably varies depending on the tissue involved. Disruption to the functional integrity of blood vessels will inevitably alter the function of the organ supplied by these vessels. Thus, in the present study, we directed our attention to the effects of ROS on porcine coronary artery, in terms of muscular and endothelial functions, and attempted to determine characteristics of the responses of each vascular element, and to clarify the specific ROS involved. MATERIALS AND METHODS Preparation of Coronary Arterial RingsPorcine hearts, immersed in ice-cooled saline, were transported to the laboratory from an abattoir, usually within 1 h of death. The left anterior descending coronary artery was isolated 2-3 cm from its orig...

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Section: Fig 5 Inhibitory Effects Of Various Ros Scavengers On Dysfmentioning

confidence: 90%

“…[12][13][14][15][16][17] In these studies, ROS scavengers are used to clarify the species of ROS involved in these effects. Among the various biological effects attributed to ROS, vascular endothelial dysfunction has consistently been noted, irrespective of the preparations used.…”

mentioning

confidence: 99%

Sustained Contraction and Endothelial Dysfunction Induced by Reactive Oxygen Species in Porcine Coronary Artery

Ishihara

Sekine

Hatano

et al. 2008

Biological & Pharmaceutical Bulletin

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“…On the other hand, under a resting state, H 2 O 2 induces tension development of vascular smooth muscle through its action on numerous cellular mechanisms [5][6][7][8][9]. Recently, Pelaez et al [9] reported that skinned (cell membrane permeabilized) muscle slowly contracted in response to H 2 O 2 in the absence of Ca 2ϩ , indicating that H 2 O 2 directly acts on the contractile proteins in vascular smooth muscle.…”

mentioning

confidence: 99%

Effects of Hydrogen Peroxide on Contraction of Skinned Aorta from Guinea Pigs

Sakurai

Watanabe²,

Yamashina

et al. 2003

JJP

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“…relaxant or contractile effects on endothelium through activating different pathways and / or modifying mem-http://bmbreports.org brane permeability to macromolecules (1,10,(13)(14)(15).…”

Section: O2mentioning

confidence: 99%

The bimodal regulation of vascular function by superoxide anion: role of endothelium

2008

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Reactive oxygen species (ROS) are implicated in vascular homeostasis. This study investigated whether O2. , the foundation molecule of all ROS, regulates vasomotor function. Hence, vascular reactivity was measured using rat thoracic aortas exposed to an O2 . generator (pyrogallol) which dose-dependently regulated both α-adrenergic agonist-mediated contractility to phenylephrine and endothelium-dependent relaxations to acetylcholine. Pyrogallol improved and attenuated responses to acetylcholine at its lower (10 nM -1 μM) and higher (10 -100 μM) concentrations, respectively while producing the inverse effects with phenylephrine

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Differential contractile actions of reactive oxygen species on rat aorta: Selective activation of ATP receptor by H2O2

Cited by 30 publications

References 19 publications

Sustained Contraction and Endothelial Dysfunction Induced by Reactive Oxygen Species in Porcine Coronary Artery

Sustained Contraction and Endothelial Dysfunction Induced by Reactive Oxygen Species in Porcine Coronary Artery

Effects of Hydrogen Peroxide on Contraction of Skinned Aorta from Guinea Pigs

The bimodal regulation of vascular function by superoxide anion: role of endothelium

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