Differential control of growth, cell cycle progression, and expression of NF-κB in human breast cancer cells MCF-7, MCF-10A, and MDA-MB-231 by ponicidin and oridonin, diterpenoids from the chinese herb Rabdosia rubescens

Hsieh, Tze Chen; Wijeratne, E. Kithsiri; Liang, Jing; Gunatilaka, A. Leslie; Wu, Joseph M.

doi:10.1016/j.bbrc.2005.09.040

Cited by 138 publications

(102 citation statements)

References 28 publications

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“…Cells were incubated with 0, 5 or 10 µl/ml water or ethanolic extracts of LZ for 1 and 3 days. Control and treated cells were harvested by trypsinization and cell numbers were determined using a hemocytometer (22)(23)(24). Harvested cells were washed twice with PBS, and pellets were stored at -80˚C for further analysis.…”

Section: Preparation Of Aqueous and Ethanolic Extracts Of Lzmentioning

confidence: 99%

“…Harvested cells were washed twice with PBS, and pellets were stored at -80˚C for further analysis. The colony formation assay was performed as described previously with some modification (23). Cells (800-1200 cells/ml, 2 ml/well) were added to 6-well tissue culture plates containing varying concentrations of water or ethanolic LZ extracts, followed by an additional 8 days of incubation to allow colonies to form.…”

Section: Preparation Of Aqueous and Ethanolic Extracts Of Lzmentioning

confidence: 99%

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Suppression of proliferation and oxidative stress by extracts of Ganoderma lucidum in the ovarian cancer cell line OVCAR-3

Hsieh

2011

Int J Mol Med

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Abstract. Lingzhi (LZ) is a medical mushroom also known as Ganoderma lucidum, which has been used in traditional Chinese medicine for more than 4,000 years and moreover, due to its presumed health benefits and apparent absence of side-effects it has also been widely consumed as a dietary supplement by cancer patients and by individuals diagnosed with various chronic diseases. The reported benefits of Ganoderma lucidum may be largely ascribed to its biologically active constituent polysaccharides and triterpenes known as ganoderic acids having structural similarity to steroid hormones. Laboratory studies have shown that Ganoderma lucidum enhances immune functions and also inhibits growth of various cancer cells both in vitro and in vivo. However, the mechanism by which Ganoderma lucidum exerts its chemopreventive activities remains unknown. In this study, we investigated whether Ganoderma lucidum elicits its anti-tumor effects by suppressing cell growth and inducing antioxidative/detoxification activity in human ovarian OVCAR-3 cells. The results showed that Ganoderma lucidum inhibits cell growth and disruption of cell cycle progression via down regulation of cyclin D1. Chemopreventive activities elicited by Ganoderma lucidum were demonstrated by the induction of antioxidant SOD and catalase as well as the phase II detoxification enzyme NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase P1 (GSTP1) via the Nrf2 mediated signaling pathway known to provide chemoprotection against carcinogenicity. These findings indicate that Ganoderma lucidum possesses chemopreventive potential contributing to its overall health effects and further suggest that Ganoderma lucidum may have clinical applications as an adjunct supplementary agent in chemotherapy.

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Section: Preparation Of Aqueous and Ethanolic Extracts Of Lzmentioning

confidence: 99%

Section: Preparation Of Aqueous and Ethanolic Extracts Of Lzmentioning

confidence: 99%

Suppression of proliferation and oxidative stress by extracts of Ganoderma lucidum in the ovarian cancer cell line OVCAR-3

Hsieh

2011

Int J Mol Med

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“…Oridonin, the most biologically active molecule purified from RRE, has been studied extensively for its effects on breast cancer (12,13), leukemia (14), cervical cancer (15), melanoma (16)(17)(18) and prostate cancer (19). Oridonin has previously been shown to inhibit the proliferation of a wide variety of human cancer cells, including prostate (lncap, du-145 and pc3), breast (mcF-7 and mda-mB-231) and non-small cell lung (nci-h520, nci-h460 and nci-h1299) cancers, acute promyelocytic leukemia (nB4) and glioblastoma multiforme (u118 and u138) (11).…”

Section: Discussionmentioning

confidence: 99%

Genomic and in vivo evidence of synergy of a herbal extract compared to its most active ingredient: Rabdosia rubescens vs. oridonin

Wong

Zhang

Kesler

et al. 2010

Experimental and Therapeutic Medicine

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Abstract. Rabdosia rubescens is a herbal root extract of traditional Chinese medicine (TCM) used to treat inflammatory diseases and oral cancers. a key principle of tcm is that multiple ingredients in a plant extract are more effective and less toxic than a single purified active ingredient or a purified drug derived from a plant product. Rabdosia rubescens extract (RRE) contains terpenoids and flavonoids, but the most active ingredient within the extract attributed to the inhibition of cancer is the kaurene diterpene, oridonin. in order to research synergy with a complete plant extract, the effects of rrE on the inhibition of prostate cancer cell proliferation were compared to the effects of pure oridonin alone in vitro. three groups of 8 Scid mice bearing human prostate cancer xenografts (lapc-4) were administered either rrE containing 0.02 mg/g oridonin, pure oridonin at a dose of 0.02 mg/g, or pure oridonin at a dose of 0.1 mg/g, by gavage for 5 days/week for 4 weeks. rrE and pure oridonin at 0.1 mg/g inhibited tumor growth to a similar extent, while oridonin at a dose of 0.02 mg/g did not. Therefore, in comparison to RRE, five times more pure oridonin was required to obtain equivalent prostate xenograft growth inhibition. Since the nuclear factor-κB signaling pathway and inflammation are implicated in prostate carcinogenesis, gene microarray analysis was conducted and demonstrated activation of genes by rrE that was not evident with oridonin treatment alone. this study demonstrated that genomic methods and xenograft studies are capable of demonstrating the benefits of the synergy of whole plant extracts rather than active ingredients isolated and purified as drugs. IntroductionBotanical extracts are used for the prevention and treatment of common conditions by 80% of the world's population according to estimates made by the World health Organization (1). drugs are produced from botanical sources by isolation and purification of the most active ingredients, while other substances in the raw fraction are separated. Synergistic interactions of mixtures of bioactive constituents and related substances or analogs in plant extracts have been proposed to occur with the most active ingredient. these interactions help explain the improved effectiveness of extracts containing multiple ingredients compared to drugs developed from single constituents.We previously studied the action and metabolism of chinese red yeast rice (Monascus purpureus Went) (crYr), a dietary supplement containing monacolins, one of which (monacolin K) is identical in structure to the statin drug lovastatin, and to unsaturated fatty acids and phytosterols capable of lowering low-density lipoprotein (ldl) cholesterol in humans (2-5). the apparent bioactivity of crYr containing 6 mg of Monacolin K was equivalent to 20 mg of purified lovastatin. moreover, crYr is well tolerated in patients who are intolerant to statin drugs (6), suggesting that the lower amounts of active substances, enhanced action and more complete metabolism in comparison to a d...

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“…1), a tetracycline diterpenoid firstly identified in the year of 1967 and subsequently synthesized in the year of 1973, has various pharmacological effects such as anti-inflammation, anti-bacteria, and anti-tumor ones (Fujita et al 1976;Li et al 2011). Oridonin shows remarkable growth inhibition and apoptosis induction activities against a broad spectrum of tumors (Hsieh et al 2005;Huang et al 2008;Kang et al 2010;Zhou et al 2007). Also, it was reported to suppress melanoma and highly metastatic breast cancer cells migration and invasion (Ren et al 2006;Wang et al 2013), although the mechanisms remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Oridonin suppress cell migration via regulation of nonmuscle myosin IIA

Sun

Zhao

et al. 2014

Cytotechnology

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Oridonin, which is isolated from Chinese herb Rabdosia rubescens (Hemsl.) Hara, has been implicated in regulation of tumor cell migration and invasion. In this study, treatment with oridonin enhanced the phosphorylation of myosin regulatory light chain (T18/S19) that regulates the ATPase activity of myosin IIA. Meanwhile, stress fibers were significantly more prominent after oridonin incubation, which impaired cell migration in transwell migration assays. All of these effects may be caused by the decreased interaction between myosin IIA and myosin phosphatase complex, but not kinases. Our data provide clear evidence of this novel pharmacological function for oridonin in treating cancer cell migration.

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Differential control of growth, cell cycle progression, and expression of NF-κB in human breast cancer cells MCF-7, MCF-10A, and MDA-MB-231 by ponicidin and oridonin, diterpenoids from the chinese herb Rabdosia rubescens

Cited by 138 publications

References 28 publications

Suppression of proliferation and oxidative stress by extracts of Ganoderma lucidum in the ovarian cancer cell line OVCAR-3

Suppression of proliferation and oxidative stress by extracts of Ganoderma lucidum in the ovarian cancer cell line OVCAR-3

Genomic and in vivo evidence of synergy of a herbal extract compared to its most active ingredient: Rabdosia rubescens vs. oridonin

Oridonin suppress cell migration via regulation of nonmuscle myosin IIA

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