Differential CRABP-II and FABP5 expression patterns and implications for medulloblastoma retinoic acid sensitivity

Zhang, Song; Liu, Huan; Li, Hong; Wu, Mo‐Li; Yu, Yang; Li, FengZhi; Cheng, Xiaoxin

doi:10.1039/c8ra00744f

Cited by 6 publications

(3 citation statements)

References 33 publications

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“…Secondly, there were increased levels of CRAPB2 and FABP5, retinoid binding proteins essential for carrying the RA to their nuclear receptors. In medulloblastoma, it has been shown that the levels of these retinoid binding proteins can lead to either proliferation when FABP5 levels are above CRABP2 levels as shown in our study or to growth inhibition (when CRAPB2 > FABP5) 23 , 24 . While both proteins were upregulated by the retinoids, FABP5 was found to be at higher increased levels than CRABP2, suggesting an increased proliferation.…”

Section: Discussionsupporting

confidence: 60%

One-year longitudinal study of the stratum corneum proteome of retinol and all-trans-retinoic acid treated human skin: an orchestrated molecular event

Abed¹,

Foucher²,

Bernard³

et al. 2023

Sci Rep

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Topically applied all-trans-retinoic acid (RA) is a gold-standard anti-aging molecule used in dermatology. As its cosmetic counterpart used in anti-aging, Retinol (ROL) is also a known metabolic precursor of RA. Despite this metabolic link, they haven’t been compared exhaustively in vivo at a mechanistic level. Therefore, to highlight the effect of a topical application of both molecules on in vivo skin, we undertook a longitudinal 1-year study and performed an untargeted proteomic analysis to get a more holistic view on the underlying biological mechanisms of action. The generation of the temporal proteomics signatures of retinol and all-trans-retinoic acid reveals the impact of these molecules on biological functions related to the aging of skin. New biological functions impacted by retinoids were discovered: glycan metabolism and protein biosynthesis. In addition, the temporal analysis reveals highest modulations at early time points while the physical measures, such as epidermal thickening, was mostly observed at the latest time point, demonstrating a strong time lapse between molecular and morphological impacts. Finally, these global temporal signatures could be used to identify new cosmetic compounds of interest.

show abstract

Section: Discussionsupporting

confidence: 60%

One-year longitudinal study of the stratum corneum proteome of retinol and all-trans-retinoic acid treated human skin: an orchestrated molecular event

Abed¹,

Foucher²,

Bernard³

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Secondly, there were increased levels of CRAPB2 and FABP5, retinoid binding proteins essential for carrying the retinoids to their nuclear receptors. In medulloblastoma, it has been shown that the levels of these retinoid binding proteins can lead to either proliferation when FABP5 levels are above CRABP2 levels as shown in our study or to growth inhibition (where CRAPB2>FABP5) [3,33] . While both proteins were upregulated by the retinoids, FABP5 was found to be at higher increased levels than CRABP2, suggesting an increased proliferation.…”

Section: Discussionsupporting

confidence: 59%

One-year longitudinal study of the stratum corneum proteome of retinol and all trans retinoic acid-treated human skin: an orchestrated molecular event

Abed

Foucher

Bernard

et al. 2023

Preprint

View full text Add to dashboard Cite

Topically applied all trans retinoic acid (RA) is a gold-standard anti-aging molecule used in dermatology. As its cosmetic counterpart used in anti-aging, Retinol (RO) is also a known metabolic precursor of RA. Despite this metabolic link, they haven’t been compared exhaustively in vivo at a mechanistic level. Therefore, to highlight the effect of a topical application of both molecules on in vivoskin, we undertook a longitudinal 1-year study and performed an untargeted proteomic analysis to get a more holistic view on the underlying biological mechanisms of action. The generation of the temporal proteomics signatures of retinol and retinoic acid reveals the impact of these molecules on biological functions related to the aging of skin. New biological functions impacted by retinoids were discovered: glycan metabolism and protein biosynthesis. In addition, the temporal analysis reveals highest modulations at early time points while the physical measures, such as epidermal thickening, was only observed at the latest time point, demonstrating a strong time lapse between molecular and morphological impacts. Finally, these global temporal signatures could be used to identify new cosmetic compounds of interest.

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“…In our study, it was upregulated in sPV compared to mPV, which agrees with previous work showing higher serum levels of psoriasin in patients with severe psoriasis 9 and a reduction after treatment with biological agents 15 . KRT6A is one of the stress keratins that regulate keratinocyte differentiation and correlation to proteins that participate in EGFR and retinoic acid signalling 22,23 . Our results demonstrated that it was upregulated in DIA but downregulated in the PRM test, which the conclusions of the previous study can explain that individual stress keratin genes are associated with partially distinct gene networks 24 .…”

Section: Discussionmentioning

confidence: 99%

Cross-sectional study of proteomic differences between moderate and severe psoriasis

Wu,

Cen,

Xie

et al. 2024

Preprint

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Although an ongoing understanding of psoriasis vulgaris (PV) pathogenesis, little is known about the proteomic differences between moderate and severe psoriasis. In this cross-sectional study, we evaluated the proteomic differences between moderate and severe psoriasis using data-independent acquisition mass spectrometry (DIA-MS). 173 differentially expressed proteins (DEPs) were significantly differentially expressed between the two groups. Among them, 85 proteins were upregulated, while 88 were downregulated (FC ≥ ± 1.5, P < 0.05). Eighteen DEPs were mainly enriched in the IL − 17 signalling pathway, Neutrophil extracellular trap formation, Neutrophil degranulation and NF − kappa B signalling pathway, which were associated with psoriasis pathogenesis. Ingenuity pathway Analysis (IPA) identified TNF and TDP53 as the top upstream up-regulators, while Lipopolysaccharide and YAP1 were the top potential down-regulators. The main active pathways were antimicrobial peptides and PTEN signalling, while the inhibitory pathways were the neutrophil extracellular trap pathway, neutrophil degranulation, and IL-8 signalling. 4D-parallel reaction monitoring (4D-PRM) suggested that KRT6A were downregulated in severe psoriasis. Our data identify Eighteen DEPs as biomarkers of disease severity, and are associated with IL − 17 signalling pathway, Neutrophil extracellular trap formation, NF − kappa B signalling pathway, and defence response to the bacterium. Targeting these molecules and measures to manage infection may improve psoriasis's severity and therapeutic efficacy.

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Differential CRABP-II and FABP5 expression patterns and implications for medulloblastoma retinoic acid sensitivity

Abstract: This study demonstrates the highly variable expression patterns of CRABP-II and FABP5 in medulloblastoma cells and their correlation with RA sensitivities.

Cited by 6 publications

References 33 publications

One-year longitudinal study of the stratum corneum proteome of retinol and all-trans-retinoic acid treated human skin: an orchestrated molecular event

One-year longitudinal study of the stratum corneum proteome of retinol and all-trans-retinoic acid treated human skin: an orchestrated molecular event

One-year longitudinal study of the stratum corneum proteome of retinol and all trans retinoic acid-treated human skin: an orchestrated molecular event

Cross-sectional study of proteomic differences between moderate and severe psoriasis

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