Differential cytotoxic activity of Quercetin on colonic cancer cells depends on ROS generation through COX-2 expression

Raja, S; Rajendiran, Vijayabharathi; Kasinathan, Nirmal Kumar; Amrithalakshmi, P; Venkatabalasubramanian, Sivaramakrishnan; Murali, Malliga Raman; Devaraj, Halagowder; Devaraj, S. Niranjali

doi:10.1016/j.fct.2017.05.006

Cited by 80 publications

(53 citation statements)

References 46 publications

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“…Cells treated with drugs (indomethacin, juglone, and combination of indomethacin and juglone) not only exhibited decreased expression of antiapoptotic Bcl2, but also showed an increased expression of proapoptotic proteins, which is in agreement with previous reports. [44] The activation of the Notch/ NICD signaling pathway causes the accumulation, transcription, and overexpression of its target gene Hes1, which plays a key role in tumorogenesis by maintaining stemness and also involved in cell proliferation, differentiation, and apoptosis. Previous reports have shown that the activated inflammatory cytokine NFκB may trigger the Wnt/βcatenin pathway, which could interact with the Notch/NICD signaling pathway to mediate cancer initiation and progression.…”

Section: Discussionmentioning

confidence: 99%

Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells

Seetha

Devaraj

Sudhandiran

2020

J Biochem & Molecular Tox

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Colorectal cancer (CRC) is the third most common fatal cancer. Indomethacin, a nonsteroidal anti-inflammatory drug, is known to reduce the occurrence of CRC.This study evaluated the potential anticolon cancer effects of juglone (5-hydroxy-1,4-naphthoquinone) in combination with indomethacin. Human colon adenocarcinoma cells (HT29) were subjected to treatment with indomethacin, juglone, and a combination of both. Morphological analysis, cell cycle regulation, and dual staining using acridine orange and ethidium bromide in control and treated cells revealed the apoptotic potential of these compounds. Bcl2 and inflammatory molecules (tumor necrosis factor-α, nuclear factor kappa B, and Cox-2) were found to be decreased with a concomitant increase in the expression of proapoptotic molecules (Bad, Bax, cytochrome c, and PUMA) as a result of the molecular regulation of Wnt, Notch, and peroxisome proliferator-activated receptor-γ signaling. Treatment with juglone was not as effective as with indomethacin; however, a combination of both was shown to be more effective, suggesting that juglone may be considered for therapeutic intervention of colon cancer.

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Section: Discussionmentioning

confidence: 99%

Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells

Seetha

Devaraj

Sudhandiran

2020

J Biochem & Molecular Tox

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“…This may explain the increase in micronuclei formation obtained after treatments with Q and E since both present a flavonoid structure with a catechol group in the B ring that gives them pro-oxidant capacities, even at the low concentrations used in this study. When reacted with the superoxide radical, this can lead to the generation of hydrogen peroxide, thus increasing its cytotoxic capacity [33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

Radioprotective and Antimutagenic Effects of Pycnanthus angolensis Warb Seed Extract against Damage Induced by X rays

Achel

Alcaraz-Saura

Castillo

et al. 2019

JCM

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Although different studies have demonstrated different applications of Pycnanthus angolensis extracts in traditional African and Asian medicine, its possible antimutagenic or genoprotective capacities have never been explored. We studied these capabilities of Pycnanthus angolensis seed extract (PASE) by means of the two micronucleus assays, determining the frequency of micronucleus (MN) yield in mouse bone marrow (in vivo) and in human lymphocytes blocked by cytochalasin B (in vitro). PASE exhibited a significant genoprotective capacity (p < 0.001) against X-rays with a protection factor of 35% in both in vivo and in vitro assays. Further, its radioprotective effects were determined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-tetrazolium bromide (MTT) cell viability test in two cell lines: one being radiosensitive (i.e., human prostate epithelium (PNT2) cells) and the other being radioresistant (i.e., B16F10 melanoma cells). In the radiosensitive cells, PASE showed a protection factor of 35.5%, thus eliminating 43.8% of X-ray-induced cell death (p < 0.001) and a dose reduction factor of 2.5. In the radioresistant cells, a protection factor of 29% (p < 0.001) with a dose reduction factor of 4 was realized. PASE elicited a greater radioprotective capacity than the substances currently used in radiation oncology and, thus, could be developed as a nutraceutical radioprotectant for workers and patients exposed to ionizing radiation.

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“…Additionally, well-known flavonoid quercetin, a plant flavonol, induced apoptosis in gastric, bladder, glioblastoma, breast, lung, colon, ovarian, cervical, liver, and pancreatic cancer cells (Balakrishnan et al, 2017;H. Li & Chen, 2018;Lin et al, 2017;Liu et al, 2017;Nwaeburu, Abukiwan, Zhao, & Herr, 2017;Raja et al, 2017;Ren et al, 2017;Roomi, Kalinovsky, Rath, & Niedzwiecki, 2017;Tao et al, 2017;E. X. Wang et al, 2017).…”

Section: Flavonoids: Cancer Therapymentioning

confidence: 99%

Leukemia therapy by flavonoids: Future and involved mechanisms

Saraei

Marofi

Naimi

et al. 2018

Journal Cellular Physiology

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Flavonoids are a varied family of phytonutrients (plant chemicals) usually are detected in fruits and vegetables. In this big family, there exist more than 10,000 members that is separated into six chief subtypes: isoflavonols, flavonoenes, flavones, flavonols, anthocyanins, and chalcones. The natural compounds, such as fruits, have visible positive effects in regulating of survival involved signaling pathways that performance as the regulator of cell survival, growth, and proliferation. Researchers have established that commonly consumption up flavonoids decreases incidence and development risk of certain cancers, especially leukemia. Flavonoids have been able to induce apoptosis and stimulate cell cycle arrest in cancer cells via different pathways. Similarly, they have antiangiogenesis and antimetastasis capability, which were shown in wide ranges of cancer cells, particularly, leukemia. It seems that flavonoid because of their widespread approval, evident safety and low rate of side effects, have hopeful anticarcinogenic potential for leukemia therapy. Based on the last decade reports, the most important acting mechanisms of these natural compounds in leukemia cells are stimulating of apoptosis pathways by upregulation of caspase 3, 8, 9 and poly ADP‐ribose polymerase (PARP) and proapoptotic proteins, particularly Bax activation. As well, they can induce cell cycle arrest in target cells not only via increasing of activated levels of p21 and p53 but also by inhibition of cyclins and cyclin‐dependent kinases. Furthermore, attenuation of neclear factor‐κB and signal transducer and activator of transcription 3 activation, suppression of signaling pathway and downregulation of intracellular antiapoptotic proteins are other significant antileukemic function mechanism of flavonoids. Overall, it appears that flavonoids are promising and effective compounds in the field of leukemia therapy. In this review, we tried to accumulate and revise most promising flavonoids and finally declared their major working mechanisms in leukemia cells.

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Differential cytotoxic activity of Quercetin on colonic cancer cells depends on ROS generation through COX-2 expression

Cited by 80 publications

References 46 publications

Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells

Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells

Radioprotective and Antimutagenic Effects of Pycnanthus angolensis Warb Seed Extract against Damage Induced by X rays

Leukemia therapy by flavonoids: Future and involved mechanisms

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