Differential development of odorant receptor expression patterns in the olfactory epithelium: A quantitative analysis in the mouse septal organ

Tian, Huikai; Ma, Minghong

doi:10.1002/dneu.20612

Cited by 24 publications

(18 citation statements)

References 42 publications

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“…This trend was not continued however as I7 was amongst the lower expressing ORs during maturity (via microarray), reaching a plateau around PND20. This result of unique expression profiles is in agreement with the developmental profile of nine ORs developmentally characterized in the septal organ (Tian and Ma, 2008). …”

Section: Discussionsupporting

confidence: 89%

Chromosomal Location-Dependent Nonstochastic Onset of Odor Receptor Expression

Rodriguez‐Gil¹,

Treloar²,

Zhang³

et al. 2010

J. Neurosci.

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As odorant receptors (ORs) are thought to be critical determinants of olfactory sensory neuron (OSN) axon targeting and organization, we examined the spatiotemporal onset of mice ORs expression from the differentiation of OSNs in the olfactory placode to an aging olfactory epithelium. ORs were first detected in the placode at embryonic day 9 (E9), at the onset of OSN differentiation but before axon extension. By E13, 22 of 23 ORs were expressed. Onset of individual OR expression was diverse; levels and patterns of expression were unique for each OR. Regional distribution of ORs within zones of the olfactory epithelium appeared stable across development; adult-like patterns were observed by E13. Finally, analysis of OR expression and chromosomal location suggests that ORs are not stochastically expressed; they show evidence of coordinated expression. Collectively, these studies demonstrate that ORs are not equally represented in the "olfactome" across an animal's lifespan.

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Section: Discussionsupporting

confidence: 89%

Chromosomal Location-Dependent Nonstochastic Onset of Odor Receptor Expression

Rodriguez‐Gil¹,

Treloar²,

Zhang³

et al. 2010

J. Neurosci.

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“…The procedure for in situ hybridization was published previously [31], [32]. Briefly, digoxigenin (DIG) labeled RNA probes of the OR genes were generated using DIG RNA Labeling Kit (SP6/T7) (Roche, Indianapolis, IN).…”

Section: Methodsmentioning

confidence: 99%

Activity-Dependent Modulation of Odorant Receptor Gene Expression in the Mouse Olfactory Epithelium

Zhao

Tian

et al. 2013

PLoS ONE

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Activity plays critical roles in development and maintenance of the olfactory system, which undergoes considerable neurogenesis throughout life. In the mouse olfactory epithelium, each olfactory sensory neuron (OSN) stably expresses a single odorant receptor (OR) type out of a repertoire of ∼1200 and the OSNs with the same OR identity are distributed within one of the few broadly-defined zones. However, it remains elusive whether and how activity modulates such OR expression patterns. Here we addressed this question by investigating OR gene expression via in situ hybridization when sensory experience or neuronal excitability is manipulated. We first examined the expression patterns of fifteen OR genes in mice which underwent neonatal, unilateral naris closure. After four-week occlusion, the cell density in the closed (sensory-deprived) side was significantly lower (for four ORs), similar (for three ORs), or significantly higher (for eight ORs) as compared to that in the open (over-stimulated) side, suggesting that sensory inputs have differential effects on OSNs expressing different OR genes. We next examined the expression patterns of seven OR genes in transgenic mice in which mature OSNs had reduced neuronal excitability. Neuronal silencing led to a significant reduction in the cell density for most OR genes tested and thinner olfactory epithelium with an increased density of apoptotic cells. These results suggest that sensory experience plays important roles in shaping OR gene expression patterns and the neuronal activity is critical for survival of OSNs.

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“…To confirm that in situ OSNs can respond to MTMT, we performed perforated patch clamp recordings in the SO, which expresses SR1 and MOR244-3 in high abundance (31,32) (Fig. S5A).…”

Section: Modeling Mor244-3 Receptor-ligand Interaction With a Candidatementioning

confidence: 99%

Crucial role of copper in detection of metal-coordinating odorants

Duan

Block

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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107

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Odorant receptors (ORs) in olfactory sensory neurons (OSNs) mediate detection of volatile odorants. Divalent sulfur compounds, such as thiols and thioethers, are extremely potent odorants. We identify a mouse OR, MOR244-3, robustly responding to (methylthio)methanethiol (MeSCH 2 SH; MTMT) in heterologous cells. Found specifically in male mouse urine, strong-smelling MTMT [human threshold 100 parts per billion (ppb)] is a semiochemical that attracts female mice. Nonadjacent thiol and thioether groups in MTMT suggest involvement of a chelated metal complex in MOR244-3 activation. Metal ion involvement in thiol-OR interactions was previously proposed, but whether these ions change thiol-mediated OR activation remained unknown. We show that copper ion among all metal ions tested is required for robust activation of MOR244-3 toward ppb levels of MTMT, structurally related sulfur compounds, and other metal-coordinating odorants (e.g., strong-smelling transcyclooctene) among >125 compounds tested. Copper chelator (tetraethylenepentamine, TEPA) addition abolishes the response of MOR244-3 to MTMT. Histidine 105, located in the third transmembrane domain near the extracellular side, is proposed to serve as a copper-coordinating residue mediating interaction with the MTMTcopper complex. Electrophysiological recordings of the OSNs in the septal organ, abundantly expressing MOR244-3, revealed neurons responding to MTMT. Addition of copper ion and chelator TEPA respectively enhanced and reduced the response of some MTMTresponding neurons, demonstrating the physiological relevance of copper ion in olfaction. In a behavioral context, an olfactory discrimination assay showed that mice injected with TEPA failed to discriminate MTMT. This report establishes the role of metal ions in mammalian odor detection by ORs.

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Differential development of odorant receptor expression patterns in the olfactory epithelium: A quantitative analysis in the mouse septal organ

Cited by 24 publications

References 42 publications

Chromosomal Location-Dependent Nonstochastic Onset of Odor Receptor Expression

Chromosomal Location-Dependent Nonstochastic Onset of Odor Receptor Expression

Activity-Dependent Modulation of Odorant Receptor Gene Expression in the Mouse Olfactory Epithelium

Crucial role of copper in detection of metal-coordinating odorants

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