Differential developmental expression and cell type specificity of Dictyostelium catalases and their response to oxidative stress and UV-light

Garcia, Ma. Xenia U.; Foote, Christopher; Es, Saskia van; Devreotes, Peter N.; Alexander, Stephen; Alexander, Hannah

doi:10.1016/s0167-4781(00)00063-4

Cited by 51 publications

(38 citation statements)

References 77 publications

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“…CatC is highly similar to catalase P from the fungal human pathogen Ajellomyces (Histoplasma) capsulatus (84% identity), Ctt1 from Schizosaccharomyces pombe (61% identity), CAT1 (43) from Candida albicans (61% identity), and the peroxisomal catalase Cta1p from Saccharomyces cerevisiae (58% identity). CatC also shows high similarity to CatA (14) from the slime mold Dictyostelium discoideum (52% identity) and catalases from the strict anaerobic methanogenic archaeon Methanosarcina barkeri (52% identity) and animals (data not shown). All of these enzymes belong to the family of small-subunit (50-to 65-kDa) monofunctional catalases.…”

Section: Resultsmentioning

confidence: 95%

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Multiple Catalase Genes Are Differentially Regulated in Aspergillus nidulans

2001

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Detoxification of hydrogen peroxide is a fundamental aspect of the cellular antioxidant responses in which catalases play a major role. Two differentially regulated catalase genes, catA and catB, have been studied in Aspergillus nidulans. Here we have characterized a third catalase gene, designated catC, which predicts a 475-amino-acid polypeptide containing a peroxisome-targeting signal. With a molecular mass of 54 kDa, CatC shows high similarity to other small-subunit monofunctional catalases and is most closely related to catalases from other fungi, Archaea, and animals. In contrast, the CatA (ϳ84 kDa) and CatB (ϳ79 kDa) enzymes belong to a family of large-subunit catalases, constituting a unique fungal and bacterial group. The catC gene displayed a relatively constant pattern of expression, not being induced by oxidative or other types of stress. Targeted disruption of catC eliminated a constitutive catalase activity not detected previously in zymogram gels. However, a catalase activity detected in catA catB mutant strains during late stationary phase was still present in catC and catABC null mutants, thus demonstrating the presence of a fourth catalase, here named catalase D (CatD). Neither catC nor catABC triple mutants showed any developmental defect, and both mutants grew as well as wild-type strains in H 2 O 2 -generating substrates, such as fatty acids, and/or purines as the sole carbon and nitrogen sources, respectively. CatD activity was induced during late stationary phase by glucose starvation, high temperature, and, to a lesser extent, H 2 O 2 treatment. The existence of at least four differentially regulated catalases indicates a large and regulated capability for H 2 O 2 detoxification in filamentous fungi.Several studies indicate that reactive oxygen species play crucial roles in various aspects of cell physiology, such as cellular defense (45), life span (38), stress signaling (22), development (19), apoptosis (30), and pathology (33). The hydrogen peroxide formed during aerobic metabolism is capable of generating other reactive oxygen species, which can damage many cellular components (18). Catalases and peroxidases are the most important enzymatic systems used to degrade H 2 O 2 . There are three separate families of catalases: Mn-catalases, bifunctional catalase-peroxidases, and monofunctional, or "true," catalases. The last group is the one best characterized and corresponds to homotetrameric heme-containing enzymes present in eubacteria and eukaryotes and recently also found in the Archaea (34). Within this family of catalases, two clearly distinct classes can be recognized: the small-subunit (50-to 65-kDa) and the large-subunit (ϳ80-kDa) enzymes. The first class includes a large number of catalases from bacteria, plants, fungi, and animals. An increasing number of catalases of the second class have been identified in bacteria and filamentous fungi (5,8,13,15,(23)(24)(25)27, 37) but not in higher eukaryotes.The core sequence of the true catalases is composed of 360 to 390 amino acid resid...

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Section: Resultsmentioning

confidence: 95%

“…The (14), and M. barkeri (34). Conserved amino acids that form part of the active (*) and heme coordination (͉) sites are indicated.…”

Section: Resultsmentioning

confidence: 99%

Multiple Catalase Genes Are Differentially Regulated in Aspergillus nidulans

2001

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“…It also has been useful in the study of DNA repair [Deering, 1988;Bronner et al, 1992;Garcia et al, 2000]. An apurinic/apyrimidinic (AP) site-specific endonuclease (APEA) involved in DNA repair has been cloned and characterized from this organism [Freeland et al, 1996].…”

Section: Introductionmentioning

confidence: 99%

Dictyostelium discoideum plasmid containing an AP‐endonuclease upstream sequence: Bleomycin induction of a luciferase reporter

Ling

Guyer

Deering

2001

Environ and Mol Mutagen

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A sequence of 1624 bp 5' to the apurinic/apyrimidinic (AP) endonuclease structural gene of Dictyostelium discoideum (APEA) has been inserted upstream of the luciferase reporter gene in pVTL2, an autonomously replicating nuclear plasmid in this organism. Cells transformed with this plasmid, designated pVTL-AL, displayed strong luciferase induction during treatment with the DNA-damaging agent bleomycin. For example, a luciferase activity of 45-fold above the constitutive level was observed for 20 hours of growth in axenic medium with 0.002 U/mL of bleomycin. The response was bleomycin concentration-dependent. Cell survival was greater than 90% for all treatments. The level of luciferase expression was highly dependent on the cell growth conditions, with the greatest induction observed for stationary phase axenically-grown cells. This effect may be related to a variation of plasmid copy number with growth conditions.

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“…Cells were collected by centrifugation, washed twice in SS (10 mM NaCl, 5 mM KCl, 2.7 mM CaCl 2 ), frozen in dry ice-ethanol, and stored at Ϫ80°C. To examine other treatments for cup induction, cells in MES-HL5 were treated as described above with 80 mM MgCl 2 , MnCl 2 , or CoCl 2 or 100 mM NaCl (ionic stress), 400 mM sorbitol (osmotic stress) (38), 1 mM H 2 O 2 (oxidative stress) (18), or 30°C (heat stress) (29). In all experiments, the cells were examined microscopically for damage at the time of sampling; no lysis or other cell damage was ever detected.…”

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confidence: 99%

The Ca ²⁺ /Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development

Coukell

Moniakis

et al. 2004

Eukaryot Cell

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Differential developmental expression and cell type specificity of Dictyostelium catalases and their response to oxidative stress and UV-light

Cited by 51 publications

References 77 publications

Multiple Catalase Genes Are Differentially Regulated in Aspergillus nidulans

Multiple Catalase Genes Are Differentially Regulated in Aspergillus nidulans

Dictyostelium discoideum plasmid containing an AP‐endonuclease upstream sequence: Bleomycin induction of a luciferase reporter

The Ca ²⁺ /Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development

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Differential developmental expression and cell type specificity of Dictyostelium catalases and their response to oxidative stress and UV-light

Cited by 51 publications

References 77 publications

Multiple Catalase Genes Are Differentially Regulated in Aspergillus nidulans

Multiple Catalase Genes Are Differentially Regulated in Aspergillus nidulans

Dictyostelium discoideum plasmid containing an AP‐endonuclease upstream sequence: Bleomycin induction of a luciferase reporter

The Ca 2+ /Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development

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The Ca ²⁺ /Calcineurin-Regulated cup Gene Family in Dictyostelium discoideum and Its Possible Involvement in Development