Differential diagnosis of HTLV-I-associated myelopathy and multiple sclerosis in Iranian patients

Kitzel, B.; Turner, Rodney W.; Burchhardt, M.; Poser, S.; Hunsmann, Gerhard; Weber, Thomas

doi:10.1007/bf00180311

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…In nonendemic regions, HTLV-1 has been detected in individuals originating from endemic areas, in their sexual partners, or in intravenous drug users. In Europe, and particularly in Germany, the prevalence of HTLV-1 is generally low (0.001±0.03%) [Goubau et al, 1996] and only a few HTLV-1-associated clinical cases have been reported for Germany [Detmar et al, 1991;Kitze et al, 1992;Ellerbrok et al, 1997Ellerbrok et al, , 1998van Dooren et al, 1998]. …”

Section: Introductionmentioning

confidence: 99%

Defective human T‐cell leukaemia virus type 1 (HTLV‐1) genomes: No evidence in serologically indeterminate german blood donors but new type detected in established cell lines

Morozov¹,

Ellerbrok

Fleischer

et al. 2001

Journal of Medical Virology

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Individuals reactive in antibody screening tests (ELISA) and with one or more reactions to HTLV-1 proteins on Western blotting, but lacking the criteria of a confirmed HTLV infection, are not exceptional in regions with a low prevalence of HTLV-1/-2 infections. PCR analysis of these indeterminate samples, using "diagnostic" pol and tax sets of primers, give negative results. However, expression of HTLV-1 defective proviruses with internal deletions undetectable by PCR with diagnostic primers could have taken place. Seven German HTLV-1 ELISA-reactive blood donors, who showed reactivity also in Western blots against several viral proteins, and twenty haemophiliacs, were examined by nested PCR and/or PCR/Southern hybridisation with primers designed for detection of HTLV-1 defective proviruses. No HTLV-1-specific amplification products were obtained. However, HTLV-1 defective proviruses with large internal deletions were detected in four out of five cell lines established from symptomatic HTLV-1 cases and two in HUT-102 cells. In two amplicons, short inverted rRNA sequences between gag and env fragments of HTLV-1 defective proviruses were revealed. These results do not exclude the presence of defective HTLV-1 proviruses in individuals with indeterminate serology although this is unlikely.

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Section: Introductionmentioning

confidence: 99%

Defective human T‐cell leukaemia virus type 1 (HTLV‐1) genomes: No evidence in serologically indeterminate german blood donors but new type detected in established cell lines

Morozov¹,

Ellerbrok

Fleischer

et al. 2001

Journal of Medical Virology

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“…On the other hand, if HTLV-I is present in the Muslim population of Mashhad, its molecular characterization could provide some clues as to the origin of HTLV-I in the Middle East. The first data suggesting that HTLV-I infection is common in Muslims from the Mashhad region were obtained in the studies of Iranian immigrants in several West European countries [Kitze et al, 1992;Gabarre et al, 1993]. It was shown that a surprisingly high proportion of HTLV-I positive cases observed in Germany, France, and the UK comprised of Iranians originating from the Mashhad region.…”

Section: Discussionmentioning

confidence: 96%

“…The Israelis infected with HTLV-I were shown to be almost exclusively immigrants from the northeast of Iran, the so called Mashhadi Jews Sidi et al, 1990;Achiron et al, 1993]. Later, it was also shown that the high prevalence of HTLV-I infection is also characteristic of the Muslim population of Mashhad [Kitze et al, 1992;Gabarre et al, 1993;Vidal et al, 1994;PuccioniSohler et al, 1995;Safai et al, 1996, Farid et al, 1995. Cases of HTLV-I infection and HTLV-I-associated diseases have also been described in Iraq [Denic et al, 1990] and, recently, in Kuwait [Voevodin et al, 1995;Farah et al, 1996;Al-Mufti et al, 1996].…”

Section: Introductionmentioning

confidence: 93%

Common origin of human T-lymphotropic virus type-I from Iran, Kuwait, Israel, and La Réunion Island

Voevodin

Gessain

1997

J. Med. Virol.

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We found previously that Kuwaiti HTLV-I isolates had two nucleotide substitutions in the most frequently sequenced regions of HTLV-I genome, namely: T-->C 4783 in the pol and T-->C 6569 the env genes. These substitutions were observed rarely in other HTLV-I isolates and seemed to be good markers of the HTLV-I lineage, the "epicentre" of which was located in Mashhad, Iran. To test this hypothesis we sequenced the fragments of HTLV-I genome including sites 4783 and 6569 from seven isolates obtained from the Iranians either living in (five isolates) or originating from (two isolates) Mashhad. RFLP-based tests were also designed and used for typing of the substitutions. All seven isolates were positive for T-->C 4783 and six, from which env fragment was amplifiable, were also positive for T-->C 6569. It is highly probable that all the isolates from Mashhadi Jews belong to the same HTLV-I lineage, although they were not typed yet for the presence of T-->C 6569 substitution. Only 2 "non-Middle Eastern" HTLV-1, both from La Réunion Island were positive for both of the substitutions. Another possible member of Mashhadi lineage of HTLV-I is one isolate from southern India and two isolates from the American Indians, British Columbia, Canada. The determination of the T-->C 4783 and T-->C 6569 markers in HTLV-I isolates of different geographical/ethnic origin may be useful for the reconstruction of the routes of HTLV-I spread from the Middle East and/or Indian subcontinent to other regions of the world and, possibly, for gaining insights into the origin of HTLV-I in Asia.

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“…Iran. Sub sequently 2 Iranians with HTLV-1 myelopa thy were reported by Kitze et al [17], The mother of 1 of them tested positive for HTLV-1 virus.…”

Section: Discussionmentioning

confidence: 99%

HTLV-1 Myelopathy in Kuwait: A Report of 2 Patients and Review of Possible Links

Farah¹,

Khan

Voevodin

et al. 1996

Med Princ Pract

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Differential diagnosis of HTLV-I-associated myelopathy and multiple sclerosis in Iranian patients

Cited by 8 publications

References 16 publications

Defective human T‐cell leukaemia virus type 1 (HTLV‐1) genomes: No evidence in serologically indeterminate german blood donors but new type detected in established cell lines

Defective human T‐cell leukaemia virus type 1 (HTLV‐1) genomes: No evidence in serologically indeterminate german blood donors but new type detected in established cell lines

Common origin of human T-lymphotropic virus type-I from Iran, Kuwait, Israel, and La Réunion Island

HTLV-1 Myelopathy in Kuwait: A Report of 2 Patients and Review of Possible Links

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