Differential diagnosis of immature germ cells in semen utilizing monoclonal antibody MHS-10 to the intra-acrosomal antigen SP-10

Homyk, Mona; Anderson, Deborah J.; Wolff, Hans; Herr, John C.

doi:10.1016/s0015-0282(16)53289-0

Cited by 18 publications

(9 citation statements)

References 13 publications

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“…In our study SIAA was immunohistologically localized within round spermatids and all cells of subsequent phases of spermiogenesis. I9G9 mAb might therefore function as a probe system for diagnosing the immature germ cells in semen, as has been shown with MHS-10 mAb [40].…”

Section: Discussionmentioning

confidence: 85%

Characterization of a Monoclonal Antibody to a Human Intra-Acrosomal Antigen that Inhibits Fertilization1

Jimenez

Sion

Grizard

et al. 1994

Biology of Reproduction

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Among monoclonal antibodies (mAb) selected after the immunization of mice with human ejaculated spermatozoa, mAb I9G9 (IgG1 kappa) was found by immunoperoxidase staining to label most of the acrosome of human spermatozoa permeabilized with methanol-acetone. The antigen was poorly expressed on the surface of fresh ejaculated sperm, but was detectable on most viable sperm after 5-h incubation in medium containing human serum albumin (HSA) followed by 30-min incubation with the calcium ionophore A23187. This treatment resulted in acrosomal loss. Immunoelectron microscopy labeling with I9G9 mAb localized the antigen within the acrosome. Immunocytochemistry on testis sections showed that antigen was located in the round spermatids within the adluminal compartment of the seminiferous epithelium. Western blotting of sperm extract proteins showed that sperm intra-acrosomal (SIAA) recognized by I9G9 mAb had a polymorphism of immunogenic peptides from 16 to 35 kDa. Most of the antigenic peptides possessed an isoelectric point of approximately 5. When spermatozoa were treated with a series of protease inhibitors, the polymorphism of immunogenic peptides was reduced, suggesting that the multiple form of the antigen was due, at least in part, to proteolytic processing. In the testis, only a single peptide band of 35 kDa was detected with mAb I9G9. Studies of human tissue specificity by Western blotting showed that the epitope recognized by I9G9 mAb was present solely in ejaculated spermatozoa and the testis. I9G9 mAb did not agglutinate or immobilize sperm but inhibited the penetration of zona-free hamster ova by human sperm.

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Section: Discussionmentioning

confidence: 85%

Characterization of a Monoclonal Antibody to a Human Intra-Acrosomal Antigen that Inhibits Fertilization1

Jimenez

Sion

Grizard

et al. 1994

Biology of Reproduction

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“…The immunocytochemical approach used in the present study has been used to detect immature germ cells in human semen and to label germ cells specifically at and beyond the immature spermatid stage (15,16,18). The use of an oil lens with a NA of 1.40 allowed good optical sectioning through the labeled cells, thus facilitating the differentiation of immature spermatids from other round cells.…”

Section: Discussionmentioning

confidence: 99%

“…The identification of these cell types by light microscopy in semen smears using conventional staining has been difficult, particularly the differentiation of round spermatids from lymphocytes/monocytes, and of spermatid symplasts (with two or more nuclei in a common cytoplasm) from polymorphonuclear granulocytes. Recently, this task has been facilitated by immunocytologic techniques using monoclonal antibodies against acrosomal and leukocyte antigens (14)(15)(16)(17)(18).…”

mentioning

confidence: 99%

Immature spermatids are not prevalent in semen from men who are receiving androgen-based contraceptive regimens

Yang

Wreford

Bremner

et al. 1998

Fertility and Sterility

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“…We have estimated from data published elsewhere [44,45] that there is a 40-fold reduction in cell volume (from 1500 m 3 to 40 m 3 ) during spermatogenesis in parallel with a 5-to 6-fold reduction in mtDNA copy number. As a consequence of the cellular volume reduction, a 7-fold increase in mtDNA copy number per cubic micrometer of cell is concurrent with the higher ATP requirement during spermatozoon maturation.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial DNA Content of Human Spermatozoa1

Dı́ez-Sánchez

Ruiz‐Pesini

Lapeña

et al. 2003

Biology of Reproduction

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Sperm mitochondria play an important role in spermatozoa because of the high ATP demand of these cells. Different mitochondrial DNA (mtDNA) mutations and haplogroups influence sperm function. The mtDNA dose also contributes to genetic variability and pathology in different tissues and organs, but nothing is known about its relevance in the performance of spermatozoa. We estimated the variability in mtDNA content within a population of men. Different mtDNA:nuclear DNA ratios were characteristic of progressive and nonprogressive spermatozoa, confirming the influence of mtDNA content on sperm functionality. We also estimated that the absolute content of mtDNA was 700 and 1200 mtDNA copies per cell in progressive and nonprogressive human spermatozoa, respectively. These results suggest that a marked increase of mtDNA copy number per cell volume takes place during spermatogenesis.

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Differential diagnosis of immature germ cells in semen utilizing monoclonal antibody MHS-10 to the intra-acrosomal antigen SP-10

Cited by 18 publications

References 13 publications

Characterization of a Monoclonal Antibody to a Human Intra-Acrosomal Antigen that Inhibits Fertilization1

Characterization of a Monoclonal Antibody to a Human Intra-Acrosomal Antigen that Inhibits Fertilization1

Immature spermatids are not prevalent in semen from men who are receiving androgen-based contraceptive regimens

Mitochondrial DNA Content of Human Spermatozoa1

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