Differential diagnosis of Taenia asiatica using multiplex PCR

Geographical Distribution of Taenia asiatica and Related Species

“…The sequence difference between the 2 species was calculated as 4.6% in the cox1 and 4.1% in the cob genes. Two variant nucleotide positions (0.1% of total length) were detected in the cox1 gene among the 5 T. asiatica isolates from China, the Philippines and Korea, whereas 13 variant nucleotide positions (0.2 to 0.8% of total length) were detected in the 10 T. saginata isolates from China, Ethiopia, France, Indonesia, Japan, Korea, Laos, the Philippines, Taiwan, Thailand and Swiss [11].…”

“…Morphological examination as well as partial nucleotide sequences of mitochondrial cox1 and ITS2 (internal transcribed spacer 2) were analyzed for 68 specimens from university or institute museum collections deposited since 1935 [14]. The specimens were identified as 3 T. solium, 51 T. asiatica, and 14 T. saginata (Table 1) [11,14]. Each province in Korea exhibited 1, 2, or 3 kinds of tapeworms.…”

“…They were designed to amplify different sized products: (1) Ta4978F, specific for T. asiatica (5′ -GGG TTT AAG TTA TAA ATG TGA TGT-3′ ; nucleotides 4978 to 5001 from GenBank accession number AF445798); (2) Ts5058F, specific for T. saginata (5′ -ACT ACA TTT GGT TTG TTT TTG TAG-3 ′ ; nucleotides 5058 to 5081 from AY684274); and (3) Tso7421F, specific for T. solium (5′ -CTA GGC CAC TTA GTA GTT TAG TTA-3′ ; nucleotides 7421 to 7444 from AB086-256). The reverse primer was from highly conserved region common to all of these tapeworms: Rev7915 (5′ -CAT AAA ACA CTC AAA CCT TAT AGA-3′ ; nucleotides 5659 to 5685 from AF445-798, nucleotides 5657 to 5683 from AY684274, and nucleotides 7870 to 7895 from AB086256) [11].…”

“…Primer design for Multiplex PCR to differentiate 3 Taenia species Species-specific forward primers were designed based on the nucleotide sequences of valine transfer RNA and NADH dehydrogenase subunit 2 from Taenia species [11]. They were designed to amplify different sized products: (1) Ta4978F, specific for T. asiatica (5′ -GGG TTT AAG TTA TAA ATG TGA TGT-3′ ; nucleotides 4978 to 5001 from GenBank accession number AF445798); (2) Ts5058F, specific for T. saginata (5′ -ACT ACA TTT GGT TTG TTT TTG TAG-3 ′ ; nucleotides 5058 to 5081 from AY684274); and (3) Tso7421F, specific for T. solium (5′ -CTA GGC CAC TTA GTA GTT TAG TTA-3′ ; nucleotides 7421 to 7444 from AB086-256).…”

Spatiotemporal Expression Patterns and Antibody Reactivity of Taeniidae Endophilin B1

“…Taenia solium metacestodes and T. solium, T. saginata, and T. asiatica adults were obtained from naturally infected pigs and humans in regions in which the parasites are endemic (Tiandong County and Sanjiang Dong Autonomous County, Guangxi Province, China). The species were identified by observing specific bands for T. solium (474 bp), T. saginata (629 bp), and T. asiatica (706 bp) by multiplex PCR (18). CE2 cysts and AE masses were collected from naturally infected sheep and experimentally infected mice in Xining (Qinghai Province, China) (19,20).…”