Differential diagnosis of the honey bee trypanosomatids Crithidia mellificae and Lotmaria passim

Ravoet, Jorgen; Schwarz, Ryan S.; Descamps, Tine; Yañez, Orlando; Tozkar, Cansu Özge; Martín-Hernández, Raquel; Bartolomé, Carolina; Smet, Lina De; Higes, Mariano; Wenseleers, Tom; Schmid‐Hempel, Regula; Neumann, Peter; Kadowaki, Tatsuhiko; Evans, Jay D.; Graaf, Dirk C. de

doi:10.1016/j.jip.2015.06.007

Cited by 68 publications

(62 citation statements)

References 40 publications

(77 reference statements)

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“…This confirms earlier studies that N. apis is currently rare in Switzerland (Dainat, Evans, Chen, Gauthier, & Neumann, 2012; Retschnig, Williams, & Neumann, in revision), and also supports that L. passim is currently the predominant trypanosomatid in A. mellifera host populations (Schwarz et al., 2015). Conversely, infections with both L. passim and N. ceranae were equally common in all three apiaries, thereby confirming the widespread occurrence of these two honey bee parasites (Fries, 2010; Ravoet et al., 2013, 2015; Schwarz et al., 2015). A higher number of workers infected with N. ceranae were found in Solothurn and St. Gallen compared to those from Fribourg.…”

Section: Discussionmentioning

confidence: 99%

“…The observed infection loads of individual bees were significantly higher for L. passim compared to N. ceranae , and are well within the limits of previous reports for foraging bees (Bourgeois et al., 2010; Ravoet et al., 2013, 2015; Retschnig, Neumann, & Williams, 2014; Retschnig et al., 2015). Despite the observed high infection loads, there were no significant differences between the infection loads of both parasites in single‐ or double‐infected hosts.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, Lotmaria passim , a trypanosome intestine parasite of honey bees, has received attention (Figure 1; Ravoet et al., 2015; Schwarz et al., 2015; Arismendi, Bruna, Zapata, & Vargas, 2016). Whereas the two trypanosomatids, Crithidia mellificae and L. passim , can both infect honey bee colonies, L. passim is currently the predominant trypanosomatid in A. mellifera host populations globally (Schwarz et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Lotmaria passim, a trypanosome intestine parasite of honey bees, has received attention ( Figure 1; Ravoet et al, 2015;Schwarz et al, 2015;Arismendi, Bruna, Zapata, & Vargas, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Host sharing by the honey bee parasites Lotmaria passim and Nosema ceranae

Tritschler

Retschnig

Yañez

et al. 2017

Ecology and Evolution

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The trypanosome Lotmaria passim and the microsporidian Nosema ceranae are common parasites of the honey bee, Apis mellifera, intestine, but the nature of interactions between them is unknown. Here, we took advantage of naturally occurring infections and quantified infection loads of individual workers (N = 408) originating from three apiaries (four colonies per apiary) using PCR to test for interactions between these two parasites. For that purpose, we measured the frequency of single and double infections, estimated the parasite loads of single and double infections, and determined the type of correlation between both parasites in double infections. If interactions between both parasites are strong and antagonistic, single infections should be more frequent than double infections, double infections will have lower parasite loads than single infections, and double infections will present a negative correlation. Overall, a total of 88 workers were infected with N. ceranae, 53 with L. passim, and eight with both parasites. Although both parasites were found in all three apiaries, there were significant differences among apiaries in the proportions of infected bees. The data show no significant differences between the expected and observed frequencies of single‐ and double‐infected bees. While the infection loads of individual bees were significantly higher for L. passim compared to N. ceranae, there were no significant differences in infection loads between single‐ and double‐infected hosts for both parasites. These results suggest no strong interactions between the two parasites in honey bees, possibly due to spatial separation in the host. The significant positive correlation between L. passim and N. ceranae infection loads in double‐infected hosts therefore most likely results from differences among individual hosts rather than cooperation between parasites. Even if hosts are infected by multiple parasites, this does not necessarily imply that there are any significant interactions between them.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Host sharing by the honey bee parasites Lotmaria passim and Nosema ceranae

Tritschler

Retschnig

Yañez

et al. 2017

Ecology and Evolution

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“…Besides bacteria, there are many fungi, viruses [62], and protozoa, i.e., Apicystis bombi, Crithidia mellificae, and Lotmaria passim (Figure 1) [63][64][65]. P. larvae is a sporulated Gram-positive Bacillus that causes the American foulbrood disease in larvae.…”

Section: The Gut Microbiota As An Environmental Factor For Honeybee Hmentioning

confidence: 99%

Microorganisms in Honey

Silva¹,

Rabadzhiev²,

Eller³

et al. 2017

Honey Analysis

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Honey is a product with low water activity because of the great amount of sugars (fructose and glucose), and also it has antimicrobial compounds derived from flowers or because of its transformation process in the beehive. Despite all the honey microorganism barriers, some species of microorganisms are able to survive and may cause damage to honeybees or consumers. Techniques of pathogenic microorganism identification by DNA using PCR are recommended and required for sanitary and customs control. It is important to know the diversity of contaminating microorganisms in honey, especially due to disseminate pathogenic microorganisms in the international traded marketing. In contrast, beneficial microorganisms such as yeasts can remain latently in this product waiting for the moment in which the environment is suitable for their development. Among the beneficial bacteria found in honeybee products, we can mention some lactic acid bacteria that act as prebiotics when ingested. The microorganisms in the digestive tract of honeybees are important for their health. Thus, we present the knowledge of microbiota associated with honey from honeybees and stingless bees (Hymenoptera, Apidae) and the techniques available for the detection of microorganisms in honey.

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What proteomics has taught us about honey bee (Apis mellifera) health and disease

Arad,

Ku,

Frey

et al. 2024

Proteomics

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The Western honey bee, Apis mellifera, is currently navigating a gauntlet of environmental pressures, including the persistent threat of parasites, pathogens, and climate change – all of which compromise the vitality of honey bee colonies. The repercussions of their declining health extend beyond the immediate concerns of apiarists, potentially imposing economic burdens on society through diminished agricultural productivity. Hence, there is an imperative to devise innovative monitoring techniques for assessing the health of honey bee populations. Proteomics, recognized for its proficiency in biomarker identification and protein–protein interactions, is poised to play a pivotal role in this regard. It offers a promising avenue for monitoring and enhancing the resilience of honey bee colonies, thereby contributing to the stability of global food supplies. This review delves into the recent proteomic studies of A. mellifera, highlighting specific proteins of interest and envisioning the potential of proteomics to improve sustainable beekeeping practices amidst the challenges of a changing planet.

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Differential diagnosis of the honey bee trypanosomatids Crithidia mellificae and Lotmaria passim

Cited by 68 publications

References 40 publications

Host sharing by the honey bee parasites Lotmaria passim and Nosema ceranae

Host sharing by the honey bee parasites Lotmaria passim and Nosema ceranae

Microorganisms in Honey

What proteomics has taught us about honey bee (Apis mellifera) health and disease

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