Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze]

Sharma, Parul; Kumar, Sanjay

doi:10.1007/bf02703703

Cited by 54 publications

(26 citation statements)

References 26 publications

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“…A better understanding of the genetic basis of stress tolerance in tea is essential for enhancing the efficiency and effectiveness of tea breeding programmes. In relation to drought, dr1, dr2 and dr3 drought responsive ESTs (expressed sequence tags) from leaf tissues have been reported using the differential display technique (Sharma and Kumar 2005). In addition, 572 ESTs from young roots of drought tolerant tea cultivars have been introduced using the suppression subtractive hybridization (SSH) technique (Das et al 2012).…”

Section: Molecular Genetic Studies On Tea Plants Under Stressmentioning

confidence: 99%

Environmental and nutritional requirements for tea cultivation

Hajiboland

2017

Folia Horticulturae

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Tea (Camellia sinensis) is an important beverage crop cultivated in the tropics and subtropics under acid soil conditions. Increased awareness of the health-promoting properties of the tea beverage has led to an increase in its level of consumption over the last decades. Tea production contributes significantly to the economy of several tea-cultivating countries in Asia and Africa. Environmental constrains, particularly water deficiency due to inadequate and/or poorly distributed rainfall, seriously limit tea production in the majority of tea-producing countries. It is also predicted that global climate change will have a considerable adverse impact on tea production in the near future. Application of fertilizers for higher production and increased quality and quantity of tea is a common agricultural practice, but due to its environmental consequences, such as groundwater pollution, the rate of fertilizer application needs to be reconsidered. Cultivation of tea under humid conditions renders it highly susceptible to pathogens and pest attacks. Application of pesticides and fungicides adversely affects the quality of tea and increases health risks of the tea beverage. Organic cultivation as an agricultural practice without using synthetic fertilizers and other chemical additives such as pesticides and fungicides is a sustainable and eco-friendly approach to producing healthy tea. A growing number of tea-producing countries are joining organic tea cultivation programmes in order to improve the quality and to maintain the health benefits of the tea produced.Ke y wor d s: aluminium, global climate change, nitrogen fertilizers, organic culture, phosphorus fertilizers, soil pH, water deficiency Abbreviations: C a -atmospheric CO 2 concentration, T a -atmospheric temperature, ESTs -estimated sequence tags, N sh -harvestable shoot density, W sh -harvestable shoot weight, C i -intracellular CO 2 concentration, T l -leaf temperature, P max -maximum photosynthetic rate, T m -maximum temperature, P n -net photosynthetic rate, T o -optimum temperature, PAR -photosynthetic active radiation, ROS -reactive oxygen species, SER -shoot extension rate, SRC -shoot replacement cycle, T s -soil temperature, SWD -soil water deficit, g s -stomatal conductance, SSH technique -suppression subtractive hybridization technique, T t -threshold temperature, TE -transpiration efficiency, VPD -vapour pressure deficit, WUE -water use efficiency

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Section: Molecular Genetic Studies On Tea Plants Under Stressmentioning

confidence: 99%

Environmental and nutritional requirements for tea cultivation

Hajiboland

2017

Folia Horticulturae

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“…Drought stress (DS) was created by withholding water during the entire period of experimentation. Abscisic acid (ABA, 100 lM) and gibberellic acid (GA 3 , 50 lM) were sprayed separately and also applied to the soil (Sharma and Kumar 2005). AB and the 1st leaf combined were harvested and used for various analyses.…”

Section: Imposition Of External Cuesmentioning

confidence: 99%

Characterization of dihydroflavonol 4-reductase cDNA in tea [Camellia sinensis (L.) O. Kuntze]

Singh

Kumar

Yadav

et al. 2008

Plant Biotechnol Rep

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Tea leaves are major source of catechinsantioxidant flavonoids. Dihydroflavonol 4-reductase (DFR, EC 1.1.1.219) is one of the important enzymes that catalyzes the reduction of dihydroflavonols to leucoanthocyanins, a key ''late'' step in the biosynthesis of catechins. This manuscript reports characterization of DFR from tea (CsDFR) that comprised 1,413 bp full-length cDNA with ORF of 1,044 bp (115-1,158) and encoding a protein of 347 amino acids. Sequence comparison of CsDFR with earlier reported DFR sequences in a database indicated conservation of 69-87% among amino acid residues. In silico analysis revealed CsDFR to be a membrane-localized protein with a domain (between 16 and 218 amino acids) resembling the NADdependent epimerase/dehydratase family. The theoretical molecular weight and isoelectric point of the deduced amino sequence of CsDFR were 38.67 kDa and 6.22, respectively. Upon expression of CsDFR in E. coli, recombinant protein was found to be functional and showed specific activity of 42.85 nmol min -1 mg protein -1 . Expression of CsDFR was maximum in younger rather than older leaves. Expression was down-regulated in response to drought stress and abscisic acid, unaffected by gibberellic acid treatment, but up-regulated in response to wounding, with concomitant modulation of catechins content. This is the first report of functionality of recombinant CsDFR and its expression in tea.

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“…The genetic mechanism of adaptive responses to drought stress in plant has also been reviewed. Drought-modulated genes (dr1,dr2 and dr3) have also been identified in Camellia sinensis L. (O) Kuntze (Sharma and Kumar 2005). Drought stress caused imbalance between the generation and quenching of reactive oxygen species (ROS).…”

Section: Introductionmentioning

confidence: 99%

Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery

2008

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Water stress is a major limitation for plant survival and growth. Several physiological and antioxidative mechanisms are involved in the adaptation to water stress by plants. In this experiment, tea cultivars (TV-1, TV-20, TV-29 and TV-30) were subjected to drought stress by withholding water for 20 days followed by rehydration. An experiment was thus performed to test and compare the effect of dehydration and rehydration in growing seedlings of tea cultivars. The effect of drought stress and post stress rehydration was measured by studying the reactive oxygen species (ROS) metabolism in tea. Water stress decreased nonenzymic antioxidants like ascorbate and glutathione contents with differential responses of enzymic antioxidants in selected clones of Camellia sinensis indicating an oxidative stress situation. This was also apparent from increased lipid peroxidation, O 2 -and H 2 O 2 content in water stress imposed plants. But the oxidative damage was not permanent as the plants recovered after rehydration. Comparatively less decrease in antioxidants, higher activities of POX, GR, CAT with higher phenolic contents suggested better drought tolerance of TV-1, which was also visible from the recovery study, where it showed lower ROS level and higher recovery of antioxidant property in response to rehydration, thus proving its better recovery potential. On the other hand, highest H 2 O 2 and lipid peroxidation with decrease in phenolic content during stress in TV-29 suggested its sensitivity to drought. The antioxidant efficiency and biochemical tolerance in response to drought stress thus observed in the tested clones of Camellia sinensis can be arranged in the order as TV-30

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Differential display-mediated identification of three drought-responsive expressed sequence tags in tea [Camellia sinensis (L.) O. Kuntze]

Cited by 54 publications

References 26 publications

Environmental and nutritional requirements for tea cultivation

Environmental and nutritional requirements for tea cultivation

Characterization of dihydroflavonol 4-reductase cDNA in tea [Camellia sinensis (L.) O. Kuntze]

Variation of physiological and antioxidative responses in tea cultivars subjected to elevated water stress followed by rehydration recovery

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