1984
DOI: 10.1016/0006-2952(84)90353-8
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Differential distribution of glutathione and glutathione-related enzymes in rabbit kidney

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Cited by 557 publications
(362 citation statements)
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“…Glutathione peroxidase activity was measured according to the procedure of Mohandas et al (1984), as described by Athar & Iqbal (1998). The reaction mixture consisted of 1.44 ml phosphate buffer (0.1 M, pH 7.4), 0.1 ml EDTA (0.5 mM) , 0.1ml sodium azide (1.0 mM), 0.05 ml glutathione reductase (1.0 EU/ml), 0.1 ml GSH (1.0 mM), 0.1 ml NADPH (0.1 mM), 0.1 ml hydrogen peroxide (0.019 M), 0.025 ml and 0.05 ml of renal and hepatic PMS (10% w/v) in a total volume of 2.0 ml.…”
Section: Methodsmentioning
confidence: 99%
“…Glutathione peroxidase activity was measured according to the procedure of Mohandas et al (1984), as described by Athar & Iqbal (1998). The reaction mixture consisted of 1.44 ml phosphate buffer (0.1 M, pH 7.4), 0.1 ml EDTA (0.5 mM) , 0.1ml sodium azide (1.0 mM), 0.05 ml glutathione reductase (1.0 EU/ml), 0.1 ml GSH (1.0 mM), 0.1 ml NADPH (0.1 mM), 0.1 ml hydrogen peroxide (0.019 M), 0.025 ml and 0.05 ml of renal and hepatic PMS (10% w/v) in a total volume of 2.0 ml.…”
Section: Methodsmentioning
confidence: 99%
“…The GPx activity was calculated by the method of Mohandas et al (1984). A total of 2 ml volume consisted of 0.1 ml EDTA (1 mM), 0.1 ml sodium azide (1 mM), 1.44 ml phosphate buffer (0.1M, pH 7.4), 0.05 ml glutathione reductase (1 IU/ml), 0.05 ml reduced glutathione (1 mM), 0.1 ml NADPH (0.2 mM) and 0.01 ml H 2 O 2 (0.25 mM) and 0.1ml 10% PMS.…”
Section: Measurement Of Glutathione Peroxidase (Gpx) Activitymentioning
confidence: 99%
“…Liver lobules were dissected out, washed immediately with ice-cold saline to remove blood, and the wet weight noted and then stored at -80 °C for various oxidative stress and biochemical assays. Post-mitochondrial supernatant (PMS) was prepared using method of Mohandas et al (1984) with some modifications.…”
Section: Hepatoprotective Activitymentioning
confidence: 99%