Differential distribution of Salmonella serovars and Campylobacter spp. isolates in free-living crows and broiler chickens in Aomori, Japan

Characterization of <i>Campylobacter jejuni</i> in large-billed crows (<i>Corvus macrorhynchos</i>) in Tochigi prefecture, Japan

“…is the most frequent lineage in humans, broilers, and cattle in Japan [2,3,11,19,20,29], it was not isolated in the present study. Of the 55 C. jejuni isolates obtained in the present study, 35 (63.6%) were classified into 21 novel STs.…”

“…Although, a large number of C. jejuni isolates from humans, broilers, and cattle in Japan have been genetically characterized by MLST [2,3,11,19,20,29], the STs of C. jejuni isolates in those studies considerably differ from the C. jejuni isolates in crows captured in the present study. Although, CC21…”

“…Okamura et al [20] reported that C. jejuni isolates belonging to ST2761, ST9010, and ST1540 were obtained from free-living crows in Aomori prefecture, Japan. Among these, ST2761 was isolated in this study as well, and the remaining two were phylogenetically more related to C. jejuni isolated from crows in this study than those isolated from human campylobacteriosis (Fig.…”

“…Shyaka et al[24] collected cloacal swab samples from 139 crows (Corvus corone and Corvus macrorhynchos) in the Tokachi area of Hokkaido, Japan, from May 2010 to May 2011, and isolated Campylobacter spp., most of which were identified as C. jejuni, from 27 (20.9%) birds. Okamura et al[20] collected cecal content samples from 123 crows (78 Corvus corone, 28 Corvus macrorhynchos, and 17 unidentified species) in Aomori prefecture, Japan, from October 2012 to April 2014, and isolated Campylobacter spp. from all the samples, most of which were also identified as C. jejuni.…”

Endpoint Recombinase Polymerase Amplification (RPA) Assay for Enumeration of Thiocyanate-degrading Bacteria

“…Biological wastewater treatments, such as the activated sludge process, are widely used, and measurements of the abundance of functional (e.g., nitrifiers and denitrifiers) and pathogenic microorganisms are important for assessing the performance and efficiency of these processes (Ishii and Sadowsky, 2008;Nurmiyanto et al, 2017;Okamura et al, 2018). Various molecular tools have been developed for this purpose, including fluorescence in-situ hybridization (FISH) and polymerase chain reaction (PCR)-based assays.…”