1999
DOI: 10.1042/bj3410515
|View full text |Cite
|
Sign up to set email alerts
|

Differential DNA binding by the androgen and glucocorticoid receptors involves the second Zn-finger and a C-terminal extension of the DNA-binding domains

Abstract: The androgen and glucocorticoid hormones evoke specific in vivo responses by activating different sets of responsive genes. Although the consensus sequences of the glucocorticoid and androgen response elements are very similar, this in vivo specificity can in some cases be explained by differences in DNA recognition between both receptors. This has clearly been demonstrated for the androgen response element PB-ARE-2 described in the promoter of the rat probasin gene. Swapping of different fragments between the… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
55
0
1

Year Published

2000
2000
2022
2022

Publication Types

Select...
7
1

Relationship

1
7

Authors

Journals

citations
Cited by 111 publications
(56 citation statements)
references
References 37 publications
0
55
0
1
Order By: Relevance
“…Previous in vitro experiments have shown that the GR is not capable of binding and inducing transcription via selective AREs (6,22,23). In this study, we made a more extensive comparison of AR, PR, MR, and GR.…”
Section: Activity Of the Different Steroid Receptors On Classical Vsmentioning
confidence: 98%
See 2 more Smart Citations
“…Previous in vitro experiments have shown that the GR is not capable of binding and inducing transcription via selective AREs (6,22,23). In this study, we made a more extensive comparison of AR, PR, MR, and GR.…”
Section: Activity Of the Different Steroid Receptors On Classical Vsmentioning
confidence: 98%
“…How to distinguish selective from classical AREs in silico is still unclear, although selective AREs have a more pronounced direct repeat nature. The GR seems unable to bind selective AREs because of a limiting characteristic of the GR-DBD second zinc finger (6). Possibly, its inability to dimerize on DR3 or flexibility limitations of the lever arm described by the group of Yamamoto (39) prevents it from binding to the selective AREs.…”
Section: Evaluation Of Pspmmentioning
confidence: 99%
See 1 more Smart Citation
“…Steroid receptors bind DNA as homodimers to repeated palindromic sequences, TGTTCT, spaced by three nucleotides [241 -244]. Next to this classical element, AR interacts with other binding motifs that resemble a direct, rather than palindromic, repeat of the TGTTCT hexamer [245,246]. The physiological activities of AR are largely determined by the presence of androgens and of others cofactors, such as coactivators (CBP, SRC1 and TIF-2) and corepressors, which enhance or repress its activity [247 -249].…”
Section: Trmentioning
confidence: 99%
“…Hormonal specificity may depend, in part, on the affinity of the steroid receptor for its cognate response element. The AR binding site 2 of the probasin ARR binds differentially with AR and GR, such that it behaves as an androgen-specific response element [38,47,48]. However, when ARBS-2 is placed inside the physiologic probasin promoter context, it cooperates with another AR binding site (ARBS-1) and binds AR and GR [38].…”
Section: Sitesmentioning
confidence: 99%