Differential Effect of Antiallergic Drugs on IgE-Mediated Cutaneous Reaction in Passively Sensitized Mice

Satoh, Taku; Tahara, Eiichi; Yamada, Toshiko; Watanabe, Chie; Itoh, Takashi; Terasawa, Katsutoshi; Nagai, Hiroichi; Saiki, Ikuo

doi:10.1159/000028353

Cited by 11 publications

(10 citation statements)

References 27 publications

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“…In this study TAC did not have any effect on immediate IST reactions but it decreased some allergen‐induced late‐phase reactions in dogs with AD. These results are similar to what is reported in the literature, where no effect was noted on immediate reactions, and suppression of late‐phase and delayed reactions have been reported 13–15 . In one study where the effects of TAC ointment on cutaneous allergic reactions in mice and rats were investigated, TAC significantly suppressed delayed allergic reactions in both species, but had no effect on immediate reactions 16 .…”

Section: Discussionsupporting

confidence: 90%

Investigation on the effects of topical therapy with 0.1% tacrolimus ointment (Protopic®) on intradermal skin test reactivity in atopic dogs

Marsella

Nicklin

Saglio

et al. 2004

Veterinary Dermatology

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Tacrolimus ointment (TAC) is an effective treatment for atopic dermatitis in humans and dogs. The purposes of the present study were to evaluate the effect of 4 weeks of TAC on intradermal skin testing (IST), and in case of suppression, to investigate if reactivity returned to baseline by 2 or 4 weeks post treatment. Intradermal skin test was performed using saline, histamine, lipopolysaccharide (LPS, 0.4 mg mL(-1)), house dust (25 PNU mL(-1)) and house dust mite (1 : 40 000 w/v) at weeks 0, 4, 6 and 8 on nine dogs enrolled in a blinded, crossover, clinical trial, using 0.1% TAC or placebo once daily for 4 weeks. Reactions were evaluated at 15 min, and at 4 and 6 h. Ointment was applied after the 15-min evaluation on weeks 0 and 4. Data were analysed using the statistical software SAS System for Windows. At week 4, TAC did not affect 15-min IST, but some reactions in the TAC group were suppressed at 6 h compared to baseline. In the TAC group, 4-h IST reactivity was reduced 2 weeks after discontinuation but returned to baseline by 4 weeks. In conclusion, TAC has no effect on immediate reactions but decreased some late-phase reactions. Therefore, no withdrawal is recommended to evaluate only immediate reactions, but a 4-week withdrawal may be necessary for evaluation of late-phase reactions.

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Section: Discussionsupporting

confidence: 90%

Investigation on the effects of topical therapy with 0.1% tacrolimus ointment (Protopic®) on intradermal skin test reactivity in atopic dogs

Marsella

Nicklin

Saglio

et al. 2004

Veterinary Dermatology

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“…7) It is worth stating that Gancao inhibited IPR, LPR and vLPR in the same manner as predonisolone, but not as an anti-histaminic agent (H1 receptor antagonists). 31) A pharmacokinetical study was also necessary to clarify the bio-equivalency between the cultivated roots and concurrent medicinal Gancao. The present GA pharmacokinetic profile (Fig.…”

Section: Discussionmentioning

confidence: 99%

Pharmaceutical Evaluation of Glycyrrhiza uralensis Roots Cultivated in Eastern Nei-Meng-Gu of China

Yamamoto

Majima

Saiki

et al. 2003

Biological & Pharmaceutical Bulletin

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Recent environmentally irresponsible over-collection of wild Glycyrrhiza plants, which produce a well-known medicinal licorice (Glycyrrhizae Radix, , Gancao in Chinese and Kanzo in Japanese), is one of the factors inducing desertification. It is reported that the World Health Organization (WHO) plans to introduce Good Field Collection Practice (GFCP) to conserve the natural environment. In 2000, the Chinese government imposed restrictions on the collection of wild Glycyrrhiza plants. Gancao is most frequently used in a famous traditional Chinese medical formulary Shan-HanLun ( ) 2) and has been used for allergic-inflammatory, gastrointestinal and liver disorders in both traditional Chinese and modern medicine. 3,4) With the increasing demand for Gancao and the decline of wild Glycyrrhiza resources, cultivated Glycyrrhiza roots have attracted attention as an additional source of Gancao. This growing interest in a new cultivated source requires a clarification of the equivalency between the cultivated Glycyrrhiza roots and concurrent Gancao. We previously reported on glycyrrhizin (GL)-content in G. uralensis roots cultivated in the eastern Nei-Meng-Gu ( ) of China, which is the habitat of G. uralensis and the major source of Dongbei-Gancao ( , Tohoku-Kanzo in Japanese).5) It was the first report that the GL-content of 4-year-old G. uralensis adventitious roots 6) exceeds the Japanese Pharmacopoeia XIV (JP XIV) standard (2.5% or more). Our cultivation study has been continued and we have obtained higher GL-content and yield of roots as well as adequate ingredients-composition similar to concurrent medicinal Gancao prepared from wild G. uralensis roots.In the present study, we describe the pharmaceutical properties of the cultivated G. uralensis roots. The overall gross similarities in chemical ingredients between the cultivated roots and Dongbei-, Xibei-( , Seihoku-Kanzo in Japanese), Xinjiang-Gancao ( , Shinkyo-Kanzo in Japanese) and wild G. uralensis roots were examined by comparing the HPLC fingerprints (chromatograms) including the GL peak. The analysis of HPLC fingerprints was further examined quantitatively by hierarchical cluster analysis (HCA) and principal component analysis (PCA). In addition to the chemical study, pharmacological and pharmacokinetical studies play an important role to assure safety and effective clinical use of a new medicinal candidate. The pharmacological properties were examined using IgE-mediated triphasic skin reaction in mice.7) The plasma concentration-time profile of glycyrrhetic acid (GA), an antiallergic 8) metabolite biotransformed from GL by intestinal bacteria, was also examined. MATERIALS AND METHODSCultivated G. uralensis Roots and Comparative Samples As shown in Fig. 1, 4-and 5-year-old roots cultivated in the same field in eastern Nei-Meng-Gu as in the previous report 5) were used. The botanical origin of the cultivated plant was identified previously 5) to be G. uralensis by referring to the morphological characteristics of flowers and fruits described.9) The same Dongb...

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“…After the first PCl challenge during the development of skin lesions, NC/Nga mice transiently exhibited a triphasic cutaneous response, which included (1) an immediate hypersensitivity reaction; (2) a delayed-type hypersensitivity reaction, and (3) a very-late-phase reaction [35,36,37]. KW3110 almost completely abrogated the first and third phases but not the second phase.…”

Section: Discussionmentioning

confidence: 99%

“…Since the transient transcriptional induction of IL-4 but not IFN-γ was significantly inhibited during the first phase, the suppression of Th2 activity appeared to contribute to the inhibition of the immediate hypersensitivity response. While the reason for the inhibition of the very-late-phase reaction is currently unclear, it may be associated with an inhibitory effect on granulocyte infiltration, a major pathological feature in this phase, which encompasses inflammatory reactions commonly mediated by IgE [37,38,39]. After the third challenge, the inhibitory effect of KW3110 became much clearer over the entire time course.…”

Section: Discussionmentioning

confidence: 99%

Dietary Supplementation with Lactic Acid Bacteria Attenuates the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice in a Strain-Dependent Manner

Wakabayashi

Nariai

Takemura

et al. 2007

Int Arch Allergy Immunol

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Background: Dietary supplementation with lactic acid bacteria (LAB) is a potential approach to the prevention and manipulation of allergic diseases such as atopic dermatitis (AD). However, the influence of different bacterial strains and their immunomodulating capacities is still largely unknown. Methods: AD-like skin lesions were induced by sensitization to and repeated challenges with picrylchloride in the Th2-skewed NC/Nga mouse strain. The effects of LAB supplementation were assessed over time by monitoring clinical scores and plasma IgE levels. In some cases, mast cell infiltration, cutaneous hypersensitivity responses and cytokine mRNA expression in auricles were also examined. Additionally, cytokine production in vitro and cytokine mRNA accumulation in major lymphoid tissues were measured, comparing Lactobacillus paracasei KW3110 with L. rhamnosus GG (LGG). Results: Supplementation with KW3110 significantly reduced the development of AD-like skin lesions, accompanied by less mast cell infiltration and lower plasma IgE levels. KW3110 also suppressed immediate hypersensitivity reactions and IL-4 mRNA expression in the auricles. These preventive effects sustained when supplementation was terminated; moreover, inhibitory effects were also observed even when supplementation was initiated after the onset of symptoms. In accordance with its effects on IL-12 and IL-4 production in vitro, KW3110 prevented the emergence of clinical symptoms more effectively than LGG in vivo. Conclusions: Supplementation with KW3110 significantly attenuated the onset and exacerbation of AD-like symptoms in NC/Nga mice. The effects were more prominent than those obtained with LGG, suggesting the importance of differences between LAB strains and their immunomodulating capacity.

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Differential Effect of Antiallergic Drugs on IgE-Mediated Cutaneous Reaction in Passively Sensitized Mice

Cited by 11 publications

References 27 publications

Investigation on the effects of topical therapy with 0.1% tacrolimus ointment (Protopic®) on intradermal skin test reactivity in atopic dogs

Investigation on the effects of topical therapy with 0.1% tacrolimus ointment (Protopic®) on intradermal skin test reactivity in atopic dogs

Pharmaceutical Evaluation of Glycyrrhiza uralensis Roots Cultivated in Eastern Nei-Meng-Gu of China

Dietary Supplementation with Lactic Acid Bacteria Attenuates the Development of Atopic Dermatitis-Like Skin Lesions in NC/Nga Mice in a Strain-Dependent Manner

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