Differential effect of opioids on immunoglobulin production by lymphocytes isolated from Peyer's patches and Spleen

Carr, Daniel J.J.; Radulescu, Razvan T.; DeCosta, Brian R.; Rice, Kenner C.; Blalock, J. Edwin

doi:10.1016/0024-3205(90)90479-b

Cited by 31 publications

(15 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The characteristic inhibition curve observed for methadone was also found for naltrindole, naloxonazine, LAAM and dextromethorphan. This type of apparent immunomodulation has been reported previously for naltrindole [6,20,23], but not for the other compounds in this group. It would appear that the responses caused by this group of compounds are not classically opioid due to the high concentration at which responses are elicited, the non-stereoselectivity and methadone's opioid antagonist insensitivity.…”

Section: Discussionmentioning

confidence: 57%

Characterisation of the in vitro modulation of splenocyte proliferation by non-4,5-epoxymorphinan opioids

Hutchinson

Somogyi

2005

International Immunopharmacology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 57%

Characterisation of the in vitro modulation of splenocyte proliferation by non-4,5-epoxymorphinan opioids

Hutchinson

Somogyi

2005

International Immunopharmacology

View full text Add to dashboard Cite

“…In animal models, opium administration can be associated with alterations in a number of immune parameters such as natural killer cells activity, proliferation of lymphocytes, antibody production and interferon production(6-8). Moreover, most of these immunosuppressive effects can be blocked by opioid antagonists (9). Despite confirmed adverse effects of opium use on immune pathways and cells, its impact on pulmonary function parameters and its immune systems is already questioned.…”

Section: Introductionmentioning

confidence: 99%

Effects of Opiate Dependence Through Different Administration Routes on Pulmonary Inflammation and its Severity

Masoomi¹,

Tajoddini²,

Mohammadi³

et al. 2013

Iran Red Crescent Med J

View full text Add to dashboard Cite

BackgroundSerious health problems and socioeconomic consequences of the illicit use of opiates have been proved in both developed and developing societiesObjectivesWe aimed to evaluate" The effects of opiate addiction through different administration routes on pulmonary inflammation and its severity."Materials and MethodsOur experiments were performed on eighteen adult male Syrian golden hamsters which were allocated to one of the three groups (n = 6): control group which did not receive opiate; the first study group were administered oral opiate via stomach tube; and another study group were administered inhaled opiate. After four weeks, all hamsters were anesthetized with diethyl ether and their lung tissues were isolated for pathological assessment.ResultsSevere perivascular inflammation was detected in 33.3% of the samples with oral opiate dependence and 20% of the cases addicted to opiate through inhalation. Also, severe peribronchial inflammation was observed in only 20% of the samples addicted to inhaled opiate and was not found in the other groups. No significant differences were found in the severity of perivascular and peribronchial inflammation across the three groups. Although the mean of total inflammation scale in the subjects with oral opiate dependence (3.00 ± 1.79) was numerically higher than that in the inhaled dependence group (1.40 ± 2.60) and the controls (2.25 ± 1.26), this difference was not statistically significant.ConclusionsAdministration did not influence the appearance or severity of pulmonary inflammation in animal models addicted to opiate.

show abstract

“…The immune system is also affected by stress and there is some evidence that corticotropin may have an immunomodulatory function. It reduces immunoglobulin production by B lymphocytes and increases their growth and differentiation [2,3]. These effects are believed to be mediated via specific corticotropin receptors which are found on the lymphocyte cell surface as determined by radioligand-binding assay [4,5].…”

mentioning

confidence: 99%

“…Cell culture. H357 cells were maintained in keratinocyte growth medium (KGM) supplemented with 10% fetal calf serum and antibiotics in an humidified atmosphere containing 5% CO 2 and 95% air at 37°C [9]. Thymidine-incorporation assays were carried out using confluent, quiescent cultures of H357 cells, in six-well plates, that were incubated in KGM containing 1 µCi/ml [ 3 H]thymidine and increasing concentrations of corticotropin in the presence of 50 µmol/l 3-isobutyl-1-methylxanthine (iBuMeXan).…”

mentioning

confidence: 99%

Direct effects of corticotrophin on oral keratinocyte cell proliferation

Kapas

Hagi‐Pavli

Brown

et al. 1998

European Journal of Biochemistry

View full text Add to dashboard Cite

Corticotropin is produced by keratinocytes and may have an immunoregulatory role in oral mucosa and skin. We have investigated its effects on a human oral keratinocyte cell line and shown that corticotropin, acting via its specific receptor, stimulates a dose-dependent increase in DNA synthesis and induces cell proliferation. When cells were incubated in the presence of increasing concentrations of corticotropin, there were significant increases in intracellular cAMP levels. Corticotropin-stimulated mitogenesis and cell proliferation were attenuated by the adenylyl cyclase inhibitor SQ22,536, but were unaffected by inhibitors of protein kinase C or tyrosine kinase. These data identify corticotropin as a mitogenic regulatory peptide of keratinocytes acting via cAMP.Keywords : corticotropin; cAMP ; keratinocyte ; mitogenesis; proliferation.Corticotropin is a well characterised peptide hormone secreted by the pituitary gland which upregulates synthesis of adrenocorticosteroids through activation of adenylyl cyclase and increases in cAMP [1] and plays a key role in the response of the body to stress. The immune system is also affected by stress and there is some evidence that corticotropin may have an immunomodulatory function. It reduces immunoglobulin production by B lymphocytes and increases their growth and differentiation [2,3]. These effects are believed to be mediated via specific corticotropin receptors which are found on the lymphocyte cell surface as determined by radioligand-binding assay [4,5].It has recently become apparent that production of corticotropin is not restricted to the pituitary. Among other cell types, it is synthesised and released by human keratinocytes in response to a number of stimuli including phorbol myristate acetate, ultraviolet light and interleukin-1 [6,7]. Since keratinocytes play an important role in the regulation of the immune response in the skin and oral mucosa by releasing proinflamatory cytokines [8], these findings raise the possibility that corticotropin may also have an immunoregulatory function. However the target cell for its action is unknown. The aims of this study were threefold; to determine firstly whether corticotropin receptors are present on human oral keratinocytes, secondly the nature of the signalling system and thirdly whether corticotropin affects keratinocyte function in terms of cell proliferation.This study reports that the human oral keratinocytes, H357, express receptors of specific high affinity for corticotropin and show that corticotropin causes an increase in cAMP accumulation and induces mitogenesis and stimulates cell proliferation. Furthermore these effects are mimicked by the cell permeable cAMP analogue, dibutyryl cAMP (Bt2-cAMP). Corticotropinstimulated mitogenesis of H357 cells was abolished in the presence of SQ22,536, a specific antagonist of adenylyl cyclase. Inhibitors of protein kinase C (PKC) or tyrosine kinase were without effect on corticotropin-stimulated mitogenic events. These results identify corticotropin working directly as ...

show abstract

Differential effect of opioids on immunoglobulin production by lymphocytes isolated from Peyer's patches and Spleen

Cited by 31 publications

References 22 publications

Characterisation of the in vitro modulation of splenocyte proliferation by non-4,5-epoxymorphinan opioids

Characterisation of the in vitro modulation of splenocyte proliferation by non-4,5-epoxymorphinan opioids

Effects of Opiate Dependence Through Different Administration Routes on Pulmonary Inflammation and its Severity

Direct effects of corticotrophin on oral keratinocyte cell proliferation

Contact Info

Product

Resources

About