Differential effects of abscisic acid on desiccation tolerance and carbohydrates in three species of liverworts

Pence, Valerie C.; Dunford, Susan S.; Redella, Steven

doi:10.1016/j.jplph.2005.02.002

Cited by 36 publications

(30 citation statements)

References 31 publications

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“…1A) contain 16.5 6 3.9 ng g 21 fresh weight endogenous ABA, comparable to the levels reported by Li et al (1994). Previous reports indicated that thalli of M. polymorpha show little ABA responses and desiccation stress tolerance (Hellwege et al, 1994;Pence et al, 2005). However, we observed that submicromolar concentrations of exogenous ABA retarded the growth of its gemma, known as a stress-tolerant form (Fig.…”

Section: Discussionsupporting

confidence: 86%

“…Response of M. polymorpha to ABA M. polymorpha has been reported as a liverwort species that shows little ABA responses (Pence et al, 2005). Thus, we first examined ABA responses of M. polymorpha gametophytes by using axenic culture of thalli and gemmae.…”

Section: Resultsmentioning

confidence: 99%

“…Recent studies have indicated that ABA might play a role in stress tolerance of liverworts (Hellwege et al, 1994;Pence et al, 2005). Nevertheless, physiological roles of ABA in liverworts have been controversial for years because of unsuccessful detection of endogenous ABA (Weiler, 1979).…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that exogenous application of ABA induces desiccation tolerance of the xerophilic liverwort Exormotheca holstii (Hellwege et al, 1994). Exogenously applied ABA also induces changes in Riccia fluitans from the aqueous form to the desiccation-tolerant land form (Hellwege et al, 1996;Pence et al, 2005). These studies have suggested that the mechanisms for ABA responses are conserved in vascular and nonvascular plants.…”

mentioning

confidence: 97%

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Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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Abscisic acid (ABA) is postulated to be a ubiquitous hormone that plays a central role in seed development and responses to environmental stresses of vascular plants. However, in liverworts (Marchantiophyta), which represent the oldest extant lineage of land plants, the role of ABA has been least emphasized; thus, very little information is available on the molecular mechanisms underlying ABA responses. In this study, we isolated and characterized MpABI1, an ortholog of ABSCISIC ACID INSENSITIVE1 (ABI1), from the liverwort Marchantia polymorpha. The MpABI1 cDNA encoded a 568-amino acid protein consisting of the carboxy-terminal protein phosphatase 2C (PP2C) domain and a novel amino-terminal regulatory domain. The MpABI1 transcript was detected in the gametophyte, and its expression level was increased by exogenous ABA treatment in the gemma, whose growth was strongly inhibited by ABA. Experiments using green fluorescent protein fusion constructs indicated that MpABI1 was mainly localized in the nucleus and that its nuclear localization was directed by the aminoterminal domain. Transient overexpression of MpABI1 in M. polymorpha and Physcomitrella patens cells resulted in suppression of ABA-induced expression of the wheat Em promoter fused to the b-glucuronidase gene. Transgenic P. patens expressing MpABI1 and its mutant construct, MpABI1-d2, lacking the amino-terminal domain, had reduced freezing and osmotic stress tolerance, and associated with reduced accumulation of ABA-induced late embryogenesis abundant-like boiling-soluble proteins. Furthermore, ABA-induced morphological changes leading to brood cells were not prominent in these transgenic plants. These results suggest that MpABI1 is a negative regulator of ABA signaling, providing unequivocal molecular evidence of PP2C-mediated ABA response mechanisms functioning in liverworts.Land plants are repeatedly challenged by environmental stresses such as desiccation, salinity, and freezing, which can cause irreversible damage to intracellular structures and membranes by severe dehydration. Abscisic acid (ABA), which is known to be a phytohormone responsible for physiological control of seed maturation and germination, bud dormancy, and stomata closure, has been postulated to be involved in the development of cellular dehydration tolerance under stress conditions. In higher plants, water-stress conditions trigger a transient or sustained increase in endogenous ABA content in tissues. Increased ABA stimulates the expression of a number of stress-related genes such as those of late embryogenesis abundant (LEA) proteins, which are thought to protect cells from the damage caused by the dehydration stress (Chandler and Robertson, 1994). It has been demonstrated that various tissue-cultured cells of vascular plants, including those of angiosperms, gymnosperms, and ferns, develop desiccation and freezing tolerance upon exogenous ABA treatment, indicating that ABA plays a key role in stress tolerance at cellular

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Section: Discussionsupporting

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 97%

See 2 more Smart Citations

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

et al. 2010

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“…ABA-treatment of mesophytic thalli of different species that are not desiccation tolerant (protonemata of Funaria hygrometrica, Olaf Werner et al 1991;Marchantia polymorpha, Riccia fluitans, Hellwege et al 1994; Pallavicina lyellii, Pence et al 2005) and of non-hardened thalli of Exormotheca (Hellwege et al 1994) increases desiccation tolerance significantly.…”

Section: Desiccation Tolerancementioning

confidence: 99%

The evolution of abscisic acid (ABA) and ABA function in lower plants, fungi and lichen

Hartung¹

2010

Functional Plant Biol.

127

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Abstract. Abscisic acid (ABA) -the universal stress hormone of cormophytes -was detected in very low concentrations in almost all organisms tested from a range of cyanobacteria, algae, bryophytes, fungi and lichens and higher plants (Fig. 1). There are a few reports only on stress-induced ABA biosynthesis in cyanobacteria and algae. This extra ABA is released to the external medium. Application of external ABA has been shown to produce weak and contradicting effects on development and metabolism of algae. In most studies, extremely high concentrations of external ABA have been applied, those being far beyond any physiological concentration range. It is, therefore, extremely difficult to discuss those data satisfactorily. When organisms start to colonise terrestrial habitats (e.g. aquatic liverworts, mosses), endogenous ABA is increased even under mild drought stress, then desiccation protecting mechanisms are stimulated and the formation of terrestrial organs is induced. The same can be observed in water ferns (Marsilea) and in a range of heterophyllous angiosperms. Sporophytes of hornwort and mosses that bear true stomata, have particularly high ABA levels and their stomata respond to ABA as is the case in cormophytes, although a significant regulatory function of these stomata does not exist. Fungi produce large amounts of ABA that are released into the external medium and do not seem to have a function for the fungus. Fungal ABA, however, may be significant in associations of fungi with cyanophytes and algae (lichens), in mycorrhizal associations and in the rhizosphere of higher plants.

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Exploration of Cryo-methods to Preserve Tree and Herbaceous Fern Gametophytes

Mikuła

Makowski²,

Walters³

et al. 2010

Working With Ferns

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Differential effects of abscisic acid on desiccation tolerance and carbohydrates in three species of liverworts

Cited by 36 publications

References 31 publications

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

Evolutionarily Conserved Regulatory Mechanisms of Abscisic Acid Signaling in Land Plants: Characterization of ABSCISIC ACID INSENSITIVE1-Like Type 2C Protein Phosphatase in the Liverwort Marchantia polymorpha

The evolution of abscisic acid (ABA) and ABA function in lower plants, fungi and lichen

Exploration of Cryo-methods to Preserve Tree and Herbaceous Fern Gametophytes

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