Differential effects of chemical and mechanical colonic irritation on behavioral pain response to intraperitoneal acetic acid in mice

Martı́nez, Vicente; Coutinho, Santosh V.; Thakur, Seema; Mogil, Jeffrey S.; Taché, Yvette; Mayer, Emeran A.

doi:10.1016/s0304-3959(99)00008-1

Cited by 86 publications

(49 citation statements)

References 31 publications

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“…Second, CRD is an easily controlled stimulus of predetermined, short duration. Intraperitoneal injection of chemical irritants produces writhes that are typically counted for 30 min after injection (38) but can persist for 6 h (29). Given available alternatives, institutional review committees are increasingly hesitant to approve the use of an uncontrolled noxious stimulus.…”

Section: Discussionmentioning

confidence: 99%

Quantitative assessment and characterization of visceral nociception and hyperalgesia in mice

Kamp

Jones

Tillman

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

102

108

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Colorectal distension (CRD) is a well-characterized model of visceral nociception, which we adapted to the mouse. CRD reproducibly evoked contractions of the abdominal musculature [visceromotor response (VMR)], which was graded to stimulus intensity. The magnitude of VMR was greater in male C57BL6 and female 129S6 mice than in male 129S6 and B6.129 mice. In 129S6, C57BL6, and B6.129 mice strains, VMR was reduced dose dependently by morphine (1-10 mg/kg) and by the -opioid agonist U-69593 (0.2-2 mg/kg), although U-69593 was significantly less potent in C57BL6 mice. In additional experiments, the VMR was recorded from adult male 129S6 mice before and after intracolonic administration of various irritants. Only 30% ethanol significantly enhanced responses to CRD. The colon hyperalgesia persisted for 14 days and was associated with a significant shift of the morphine doseresponse function to the left. We believe this will be a useful model for study of visceral nociception and hyperalgesia, including studies of transgenic mice with mutations relevant to pain. colon; visceral pain; knockout mice; strain; gender; -opioid PAIN IS THE MOST COMMON REASON patients seek care from a physician, and the majority of visits are for pain of visceral origin. Current knowledge about mechanisms of pain has been derived from studies of somatic, principally cutaneous pain. Visceral pain has been less well studied, in part, because of more difficult access to visceral structures. Additionally, stimuli that are painful when applied to the skin, such as burning, crushing, and cutting, evoke no pain when applied to most visceral structures. Furthermore, there is growing evidence that the mechanisms of visceral and cutaneous pain are different (8,19). Certainly, the characteristics of these pains are different; visceral pain is diffuse, poorly localized, and referred to overlying structures (see Refs. 7 and 46 for reviews).Balloon distension of hollow organs, such as the colon, stomach, or urinary bladder produces pain in humans and quantifiable behavioral and autonomic responses in nonhuman animals. These responses have been most extensively characterized for colorectal distension (CRD) in the rat. When applied to rats at pressures comparable to those that produce pain in humans, CRD is aversive and produces tachycardia, pressor effects, contraction of the abdominal musculature, activation of afferent (sensory) fibers in the pelvic nerve, and activation of second-order neurons in the dorsal horn of the spinal cord (45,46,52).The availability of genetically modified mice has increased interest in the mouse as a nonhuman experimental animal. Numerous mice have been generated with mutations relevant to the study of pain; most have been tested in cutaneous models of nociception only. Some transgenic mice have been tested using the intraperitoneal acetic acid model of visceral nociception (writhing test); however, a limited understanding of the underlying structures and sensory neurons activated in this model confounds interpretation of resul...

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Section: Discussionmentioning

confidence: 99%

Quantitative assessment and characterization of visceral nociception and hyperalgesia in mice

Kamp

Jones

Tillman

et al. 2003

American Journal of Physiology-Gastrointestinal and Liver Physi

102

108

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“…62 The pain response was scored by counting the number of abdominal contractions during the 30 min period after the IP treatment (in blocks of 5 min) by 2 independent researchers.…”

Section: Immunohistochemistry and Quantification Of Immunepositive Simentioning

confidence: 99%

Antibiotic-induced dysbiosis alters host-bacterial interactions and leads to colonic sensory and motor changes in mice

2015

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Abbreviations: AMP, antimicrobial peptide; CB1/2, cannabinoid receptor type 1 or 2; FGD, functional gastrointestinal disorder; FISH, fluorescent in situ hybridization; GCM, gut commensal microbiota; GI, gastrointestinal; IBS, irritable bowel syndrome; iNOS, inducible nitric oxide synthase; MOR, mu-opioid receptor; NGF, nerve growth factor; PPR, pattern recognition receptor; Reg3g, regenerating islet-derived protein 3 gamma; RELMb, resistin-like molecule-b; RT-qPCR, reverse transcription quantitative polymerase chain reaction; SFB, segmented filamentous bacteria; sIgA, secretory IgA; TLR, toll-like receptor; TPH 1/2, tryptophan hydroxylase isoforms 1 or 2; TRPV1/3, transient receptor potential vanilloid types 1 or 3Alterations in the composition of the commensal microbiota (dysbiosis) seem to be a pathogenic component of functional gastrointestinal disorders, mainly irritable bowel syndrome (IBS), and might participate in the secretomotor and sensory alterations observed in these patients.We determined if a state antibiotics-induced intestinal dysbiosis is able to modify colonic pain-related and motor responses and characterized the neuro-immune mechanisms implicated in mice. A 2-week antibiotics treatment induced a colonic dysbiosis (increments in Bacteroides spp, Clostridium coccoides and Lactobacillus spp and reduction in Bifidobacterium spp). Bacterial adherence was not affected. Dysbiosis was associated with increased levels of secretory-IgA, up-regulation of the antimicrobial lectin RegIIIg, and toll-like receptors (TLR) 4 and 7 and down-regulation of the antimicrobial-peptide Resistin-Like Molecule-b and TLR5. Dysbiotic mice showed less goblet cells, without changes in the thickness of the mucus layer. Neither macroscopical nor microscopical signs of inflammation were observed. In dysbiotic mice, expression of the cannabinoid receptor 2 was up-regulated, while the cannabinoid 1 and the mu-opioid receptors were down-regulated. In antibiotic-treated mice, visceral painrelated responses elicited by intraperitoneal acetic acid or intracolonic capsaicin were significantly attenuated. Colonic contractility was enhanced during dysbiosis. Intestinal dysbiosis induce changes in the innate intestinal immune system and modulate the expression of pain-related sensory systems, an effect associated with a reduction in visceral painrelated responses. Commensal microbiota modulates gut neuro-immune sensory systems, leading to functional changes, at least as it relates to viscerosensitivity. Similar mechanisms might explain the beneficial effects of antibiotics or certain probiotics in the treatment of IBS.

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“…injection of compounds. Contortions induced by acetic acid were used as a positive control 26 . The NLP vehicle produced fewer abdominal contortions than the CLP vehicle, indicating that the animals experienced less pain upon injection of NLP.…”

Section: Discussionmentioning

confidence: 99%

Caracterização anestésica da nanoemulsão não lipídica de propofol

Sudo¹,

Bonfá²,

Trachez³

et al. 2010

Rev. Bras. Anestesiol.

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Summary: Sudo RT, Bonfá L, Trachez MM, Debom R, Rizzi MDR, Zapata-Sudo G -Anesthetic Profile of a Non-lipid Propofol Nanoemulsion. Background and objectives:The clinical use of a lipid propofol formulation causes pain during injection, allergic reactions, and bacterial growth. Propofol has been reformulated in different non-lipid presentations to reduce the incidence of adverse effects, but those changes can modify its pharmacokinetics and pharmacodynamics. In the present study, we investigate the pharmacology and toxicology of lipid propofol (CLP) and the non-lipid nanoemulsion (NLP).

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Differential effects of chemical and mechanical colonic irritation on behavioral pain response to intraperitoneal acetic acid in mice

Cited by 86 publications

References 31 publications

Quantitative assessment and characterization of visceral nociception and hyperalgesia in mice

Quantitative assessment and characterization of visceral nociception and hyperalgesia in mice

Antibiotic-induced dysbiosis alters host-bacterial interactions and leads to colonic sensory and motor changes in mice

Caracterização anestésica da nanoemulsão não lipídica de propofol

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