Differential Effects of
            <i>Bifidobacterium pseudolongum</i>
            Strain Patronus and Metronidazole in the Rat Gut

Vasquez, Nadia; Suau, Antonia; Magne, Fabien; Pochart, Philippe; Pélissier, M.A.

doi:10.1128/aem.01731-08

Cited by 19 publications

(16 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The primer sequences are presented in Table 1. The forward primer Bia339 and the reverse primer Bia788 were used to amplify variable regions 3 and 4 of the bacterial 16S rRNA genes (Vasquez et al , 2009). The forward primer Bif164 and the reverse primer Bif662 were used to amplify 16S rRNA genes of bifidobacteria.…”

Section: Methodsmentioning

confidence: 99%

“…qPCR was performed using a Mastercycler ep realplex (Eppendorf SARL, Le Pecq, France). Amplification conditions have been described previously (Vasquez et al , 2009). The specific annealing temperatures were 55 °C for total bacteria, 62 °C for bifidobacteria and 58 °C for enterobacteria.…”

Section: Methodsmentioning

confidence: 99%

“…We have demonstrated previously that oral administration of metronidazole reduced basal oxidative stress in colonic tissue of healthy rat (Pelissier et al , 2007). Moreover, in a recent study, we suggested that this antioxidant effect could be linked to the modified composition of colonic microbiota and particularly to the increased bifidobacterial populations induced by metronidazole (Vasquez et al , 2009). Considering the protective role of the mucus layer against oxidative stress, we hypothesized that metronidazole could also be involved in the modulation of the mucus layer through the alteration of intestinal microbiota.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metronidazole effects on microbiota and mucus layer thickness in the rat gut

Pélissier

Vasquez

Balamurugan

et al. 2010

FEMS Microbiology Ecology

Self Cite

View full text Add to dashboard Cite

Both mucus and mucosa-associated bacteria form a specific environment in the gut; their disruption may play a crucial role in the development of intestinal bowel disease (IBD). Metronidazole, an antibiotic used in the treatment of IBD, alters gut microbiota and reduces basal oxidative stress to proteins in colonic tissue of healthy rats. The aim of this study was to evaluate the impact of the altered microbiota due to the metronidazole on the thickness of the mucus layer. This study was performed in healthy untreated rats (control group) or rats treated by metronidazole (metronidazole-treated rats, 1 mg mL(-1) in drinking water for 7 days). Both PCR-temporal temperature gradient gel electrophoresis and quantitative PCR (qPCR) revealed an altered microbiota with an increase in bifidobacteria and enterobacteria in metronidazole-treated rats compared with control rats. Moreover, a dominant bifidobacterial species, Bifidobacterium pseudolongum, was detected. Using qPCR and FISH, we showed that bifidobacteria were also increased in the microbiota-associated mucosa. At the same time, the mucus layer thickness was increased approximately twofold. These results could explain the benefits of metronidazole treatment and warrant further investigations to define the role of bifidobacteria in the colonic mucosa.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metronidazole effects on microbiota and mucus layer thickness in the rat gut

Pélissier

Vasquez

Balamurugan

et al. 2010

FEMS Microbiology Ecology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Bifidobacteria are considered as health-promoting microorganisms due to potential beneficial roles in the intestinal tract, such as immunostimulation, reduction of growth of many potential pathogens and putrefactive bacteria, the prevention of constipation, diarrhoea and other intestinal disorders and improvement of lactose-tolerance, [9], [10], [11]. Moreover, we had previously demonstrated that bifidobacterial increase reduced basal oxidative stress in colonic tissue of healthy rats [12]. In vitro susceptibility test showed that the Bifidobacterium species isolated from the human colonic microbiota were generally sensitive to amoxicillin [13], [14], [15], [16], a beta-lactam antibiotic widely prescribed for the treatment of respiratory tract infections in adults and children.…”

Section: Introductionmentioning

confidence: 99%

Long-Term Changes in Human Colonic Bifidobacterium Populations Induced by a 5-Day Oral Amoxicillin-Clavulanic Acid Treatment

et al. 2012

Self Cite

View full text Add to dashboard Cite

The objective of this study was to assess the possible modifications due to amoxicillin-clavulanic acid (AMC) treatment on total bacteria and on Bifidobacterium species balance in human colonic microbiota. Eighteen healthy volunteers (19 to 36 years old) were given a 875/125 mg dose of AMC twice a day for 5 days. Fecal samples were obtained before and after antibiotic exposure. After total DNA extraction, total bacteria and bifidobacteria were specifically quantified using real-time PCR. Dominant species were monitored over time using bacterial and bifidobacterial Temporal Temperature Gradient gel Electrophoresis (TTGE). At the end of AMC exposure, total bacterial concentrations as well as bifidobacteria concentrations were significantly reduced compared to before AMC exposure:10.7±0.1 log10 16S rRNA gene copies/g vs 11.1±0.1 log10 (p = 0.003) and 8.1±0.5 log10 16S rRNA gene copies/g vs 9.4±0.3 log10 (p = 0.003), respectively. At the same time, the mean similarity percentages of TTGE bacteria and TTGE bifidobacteria profiles were significantly reduced compared to before AMC exposure: 51.6%±3.5% vs 81.4%±2.1% and 55.8%±7.6% vs 84.5%±4.1%, respectively. Occurrence of B. adolescentis, B. bifidum and B. pseudocatenulatum/B. catenulatum species significantly decreased. Occurrence of B. longum remained stable. Moreover, the number of distinct Bifidobacterium species per sample significantly decreased (1.5±0.3 vs 2.3±0.3; p = 0.01). Two months after AMC exposure, the mean similarity percentage of TTGE profiles was 55.6% for bacteria and 62.3% for bifidobacteria. These results clearly demonstrated that a common antibiotic treatment may qualitatively alter the colonic microbiota. Such modifications may have potential long-term physiological consequences.

show abstract

“…The AS plaque PA/PL value was significantly and negatively correlated with the Bifidobacteria count. The dominant Bifidobacteria species in the ApoE −/− mouse gut is Bifidobacterium pseudolongum (52). We make a putative opinion that TP could induce a Bifidobacteria boom, which could prevent AS development by modulating the fat metabolism.…”

Section: Discussionmentioning

confidence: 99%

Impact of the Consumption of Tea Polyphenols on Early Atherosclerotic Lesion Formation and Intestinal Bifidobacteria in High-Fat-Fed ApoE−/− Mice

et al. 2016

View full text Add to dashboard Cite

There is an increasing interest in the effect of dietary polyphenols on the intestinal microbiota and the possible associations between this effect and the development of some cardiovascular diseases, such as atherosclerosis (AS). However, limited information is available on how these polyphenols affect the gut microbiota and AS development. This study was designed to evaluate the modulation of dietary tea polyphenols (TPs) on intestinal Bifidobacteria (IB) and its correlation with AS development in apolipoprotein E-deficient (ApoE−/−) mice. Fifty C57BL/6 ApoE−/− mice were randomized into one of the five treatment groups (n = 10/group): control group fed normal diet (CK); a group fed a high-fat diet (HFD); and the other three groups fed the same HFD supplemented with TPs in drinking water for 16 weeks. The total cholesterol and low-density lipoprotein cholesterol (LDL-C) were decreased significantly (P < 0.05) after TP interference. In addition, the TP diet also decreased the plaque area/lumen area (PA/LA) ratios (P < 0.01) in the TP diet group. Interestingly, copies of IB in the gut of ApoE−/− mice were notably increased with TP interference. This increase was dose dependent (P < 0.01) and negatively correlated with the PA/LA ratio (P < 0.05). We conclude that TPs could promote the proliferation of the IB, which is partially responsible for the reduction of AS plaque induced by HFD.

show abstract

Differential Effects of Bifidobacterium pseudolongum Strain Patronus and Metronidazole in the Rat Gut

Cited by 19 publications

References 55 publications

Metronidazole effects on microbiota and mucus layer thickness in the rat gut

Metronidazole effects on microbiota and mucus layer thickness in the rat gut

Long-Term Changes in Human Colonic Bifidobacterium Populations Induced by a 5-Day Oral Amoxicillin-Clavulanic Acid Treatment

Impact of the Consumption of Tea Polyphenols on Early Atherosclerotic Lesion Formation and Intestinal Bifidobacteria in High-Fat-Fed ApoE−/− Mice

Contact Info

Product

Resources

About