Differential effects of miR-34c-3p and miR-34c-5p on the proliferation, apoptosis and invasion of glioma cells

Wu, Zhiyong; Wu, Yupeng; Tian, Ye; Sun, Xiaofang; Liu, Jixiang; Ren, Haitao; Liang, Chaohui; Song, Ligang; Hu, Hongchao; Wang, Liqun; Jiao, Baohua

doi:10.3892/ol.2013.1579

Cited by 61 publications

(63 citation statements)

References 26 publications

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“…Pattern C miRNAs seem to contribute to the df/df mouse longevity by a CR‐independent mechanism. Recent reports demonstrate tumor suppressor (Wu et al ., 2013) and neuroprotective (Minones‐Moyano et al ., 2011) functions for these miRNAs. Wu and colleagues found downregulation of miR‐34c‐3p/5p in glioma tissue.…”

Section: Discussionmentioning

confidence: 99%

“…Wu and colleagues found downregulation of miR‐34c‐3p/5p in glioma tissue. Overexpression of these two miRNAs in glioma cell lines inhibited the cell proliferation, cell cycle changes, apoptosis, and cell invasion (Wu et al ., 2013). We found that predicted targets of the pattern C GbA miRNAs are significantly enriched in the MAPK signaling pathway (Table S4).…”

Section: Discussionmentioning

confidence: 99%

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Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse

et al. 2015

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SummaryRecent evidence demonstrates that serum levels of specific miRNAs significantly change with age. The ability of circulating sncRNAs to act as signaling molecules and regulate a broad spectrum of cellular functions implicates them as key players in the aging process. To discover circulating sncRNAs that impact aging in the long‐lived Ames dwarf mice, we conducted deep sequencing of small RNAs extracted from serum of young and old mice. Our analysis showed genotype‐specific changes in the circulating levels of 21 miRNAs during aging [genotype‐by‐age interaction (GbA)]. Genotype‐by‐age miRNAs showed four distinct expression patterns and significant overtargeting of transcripts involved in age‐related processes. Functional enrichment analysis of putative and validated miRNA targets highlighted cellular processes such as tumor suppression, anti‐inflammatory response, and modulation of Wnt, insulin, mTOR, and MAPK signaling pathways, among others. The comparative analysis of circulating GbA miRNAs in Ames mice with circulating miRNAs modulated by calorie restriction (CR) in another long‐lived mouse suggests CR‐like and CR‐independent mechanisms contributing to longevity in the Ames mouse. In conclusion, we showed for the first time a signature of circulating miRNAs modulated by age in the long‐lived Ames mouse.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse

et al. 2015

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“…Apart from p27, miRNAs are one of the most common regulatory factors in NPC [49–51], and they play physiological and pathophysiological roles in cell proliferation, differentiation and apoptosis by regulating protein expression in a post-transcriptional manner [52, 53]. Wu et al [54] identified miR-30d as a tumor suppressor, which was regulated by a new AKT/FOXO/miR-30d/MTDH signaling transduction pathway.…”

Section: Discussionmentioning

confidence: 99%

DC120, a novel AKT inhibitor, preferentially suppresses nasopharyngeal carcinoma cancer stem-like cells by downregulating Sox2

Qin

Deng

et al. 2015

Oncotarget

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Side population (SP) contains cancer stem-like cells (CSLCs). In this study, we characterized SP cells from nasopharyngeal carcinoma (NPC) cell lines and found that SP cells had a higher self-renewal ability in vitro and greater tumorigenicity in vivo. The AKT pathway was activated in NPC SP cells. DC120, a 2-pyrimidyl-5-amidothiazole inhibitor of the ATP binding site of AKT, inhibited phosphorylation of FKHRL1 and GSK-3β. DC120 inhibited SP fraction, the sphere-forming ability in vitro and growth of primary xenografts as well as secondary xenografts’ tumor recurrence. This inhibition was accompanied by reduced expression of stem-related gene Sox2 due to induction of p27 and miR-30a. A combination of DC120 and CDDP more effectively inhibited NPC cells compared with monotherapy in vitro and in vivo. Clinical evaluation of DC120 is warranted.

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“…The family of miR-34, including miR-34a, b and c, has been reported to regulate numerous cellular events, including the cell cycle, cell migration and apoptosis (38,39). miR-34c has two identified mature miRNAs: miR-34c-3p and miR-34c-5p (40,41). Previous studies have indicated that miR-34c has tumor-suppressive effects, and suppresses the cell cycle primarily by induction of G1 cell cycle arrest (42).…”

Section: Discussionmentioning

confidence: 99%

Pien Tze Huang inhibits the proliferation of colorectal cancer cells by increasing the expression of miR-34c-5p

Wan

Shen

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. MicroRNAs (miRNAs) are small, short endogenous non-coding RNA that act as oncogenes or tumor suppressors, and serve an important role in various human malignant cancers, including colorectal cancer (CRC). Evidence has indicated that miRNAs regulate the expression of various genes associated with human cancer, in particular the miR-34 family. A well-known traditional Chinese formula, Pien Tze Huang (PZH), has a significant clinical effect on CRC. Previous studies have demonstrated that PZH inhibits CRC growth in vitro and in vivo via multiple mechanisms, including the induction of apoptosis, inhibition of cell proliferation and tumor angiogenesis. To further elucidate the molecular mechanisms underlying the antitumor activity of PZH, in the present study its effects on cell proliferation and miRNA expression in human colon carcinoma (HCT)-8 cell lines was examined. It was observed that treatment with PZH inhibited cell viability and upregulated the expression of miR-34c-5p in HCT-8 cells. In addition, transfection with an miR-34c-5p mimic and treatment with PZH inhibited cell survival and arrested the cell cycle between the G0/G1 and S phase in HCT-8 cells. Furthermore, PZH treatment and transfection with miR-34c-5p downregulated the expression of cyclin-dependent kinase 4 and cMyc (a promoter of cell proliferation), and increased the expression of p53, which is a promoter of apoptosis. These results suggest that PZH may suppress proliferation in CRC cells by upregulating the expression of miR-34c-5p, which provides a novel perspective for understanding the mode of action of PZH.

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Differential effects of miR-34c-3p and miR-34c-5p on the proliferation, apoptosis and invasion of glioma cells

Cited by 61 publications

References 26 publications

Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse

Circulating microRNA signature of genotype‐by‐age interactions in the long‐lived Ames dwarf mouse

DC120, a novel AKT inhibitor, preferentially suppresses nasopharyngeal carcinoma cancer stem-like cells by downregulating Sox2

Pien Tze Huang inhibits the proliferation of colorectal cancer cells by increasing the expression of miR-34c-5p

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