Differential effects of polyadenylic: polyuridylic acid and lipopolysaccharide on the generation of cytotoxic T lymphocytes.

Narayanan, P R; Sundharadas, G.

doi:10.1084/jem.147.5.1355

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…However, in the liver, poly(A:U) decreased the CFU relative to that in control mice given PBS in experiment 1, the appropriate control in this experiment; in a second experiment (experiment 3), poly(A:U) mixed with rabbit serum decreased the CFU relative to that in mice given rabbit serum alone (Table 2). Since poly(A:U) has pleiotropic effects, including direct effects on macrophages and T cells (3,19,20), it was not clear from these experiments if the observed effect is related to NK activation.…”

Section: Resultsmentioning

confidence: 95%

“…While we found no evidence for a positive role for NK cells in control of brucellosis in mice that did not receive immunopotentiators, regardless of the infection dose, our results indicated increased control of brucellosis in the livers (but not the spleens) of C57BL/10 mice infected with the high challenge dose and treated with poly(A:U). Poly(A:U) is known to affect cells other than NK cells (3,19,20), and it has been reported previously by others that the effectiveness of poly(A:U) at reducing the number of brucellae in CBA and athymic congenic mice infected with the attenuated B. abortus strain 19 (4,16) was by its macrophage-activating capacity (17). The role of poly(A:U) for facilitating increased clearance in the studies conducted here may be similar.…”

Section: Vol 63 1995mentioning

confidence: 99%

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Lack of a role for natural killer cells in early control of Brucella abortus 2308 infections in mice

1995

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Studies were conducted to determine if natural killer (NK) cells are important for early control of the virulent strain Brucella abortus 2308 following infection of mice with high or low challenge doses. Splenocytes from C57BL/10 and BALB/c mice that had been infected with the lower dose of B. abortus displayed increased cytotoxicity against YAC-1 cells during the first week after infection, while infection of C57BL/10 mice with the higher challenge dose either did not alter the level of NK cytotoxic activity or decreased it, depending upon the time postinfection. In vivo depletion of NK cells by monoclonal antibody anti-NK1.1 or polyclonal anti-asialo-GM 1 antiserum did not result in an increase in the number of brucellae recovered from the spleens or livers of the brucella-resistant C57BL/10 mice or from the spleens of the susceptible BALB/c mice during the first week after infection. Treatment of control mice with the NK-reactive antibodies, however, decreased killing of the NK-sensitive target YAC-1, indicating that the NK cell depletion regimes were effective. Our results suggest that NK cells are not crucial for early control of B. abortus 2308 even though they may be activated following infection. Further experiments indicated that treatment of C57BL/10 mice with poly(A:U) did not decrease the number of brucellae recovered from their spleens although it did decrease the CFU in livers of mice infected with the high challenge dose.

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Section: Resultsmentioning

confidence: 95%

Section: Vol 63 1995mentioning

confidence: 99%

Lack of a role for natural killer cells in early control of Brucella abortus 2308 infections in mice

1995

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“…20, 23], indicating that LPS influences T-cell responses. Furthermore, other cell-mediated reactions, including the curing of tumours [5,6] and generation of cytotoxic T cells [24] after LPS administration, are dependent on T ceiis, suggesting that LPS plays a role in the generation of tumour-immune T cells. LPS interferes with the induction of T-cell tolerance to protein antigens [27].…”

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confidence: 99%

Cellular Responses to Bacterial Lipopolysaccharide: T Cells Recognize LPS Determinants

1983

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An in vitro proliferative system was used to assess the capacity of lipopolysaccharide (LPS) to induce a specific T-cell response in mice. Sensitized T cells were generated in vivo by subcutaneous inoculation with LPS. These T cells, which were purified on nylon wool columns, were stimulated to proliferate in vitro by LPS. Results of several lines of experimentation confirmed that the responding cells were T cells. Additional experiments indicated that sensitized T cells could distinguish between LPS and ovalbumin. Finally, it was found that genetic responsiveness to the biological effects of lipid A was required for full elicitation of LPS-induced T-cell proliferation. The data were interpreted to indicate that LPS interacted with, and stimulated, antigen-specific murine T cells.

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“…Previously, LPS been shown to potentiate a variety of manifestations of cell-mediated immune responses (19,24,30). However, the mechanism by which LPS immunopotentiates protein antigens and improves their protective ability is not yet well understood.…”

Section: Discussionmentioning

confidence: 99%

Mechanism of protective immunity induced by porin-lipopolysaccharide against murine salmonellosis

Muthukkumar

Muthukkaruppan

1993

Infect Immun

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Investigations were undertaken to characterize the protective immunity induced by porin-lipopolysaccharide (LPS) against SalmoneUla typhimurium infection in mice. Mice immunized with porin-LPS showed higher levels of antiporin immunoglobulin G than mice which received porin alone. Further, T cells from porin-LPSimmunized mice showed an augmented proliferative response to porin in vitro compared with the response of T cells from porin-injected animals. The passive transfer of anti-LPS antibodies conferred significant protection (17%), while antiporin serum failed to protect mice against lethal challenge, indicating the protective ability of anti-LPS antibodies. However, the transfer of serum obtained from porin-LPS-immunized mice resulted in better protection (30%o) than did anti-LPS or antiporin antibodies alone. In contrast to LPS, monophosphoryl lipid A completely failed to induce protection against lethal infection. However, comparable to the effect of LPS, injection of porin with monophosphoryl lipid A enhanced antibody response and the protective ability of porin (81.25%). The transfer of T cells from porin-LPS-immunized mice provided higher levels of protection (47%) against lethal challenge than did T cells from porin-immunized mice (23%). The combination of T cells and serum from porin-immunized mice transferred 36% protection. However, a combination of T cells and serum from porin-LPS-immunized mice conferred the highest level of protection (92%), which was reflected by the number of survivors (100%/) in the porin-LPS-immunized group. These results demonstrate that besides the protective effect of anti-LPS antibodies, the ability of LPS to augment humoral and cell-mediated immune responses to porin confers effective protection against SalnoneUla infection.

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Differential effects of polyadenylic: polyuridylic acid and lipopolysaccharide on the generation of cytotoxic T lymphocytes.

Cited by 11 publications

References 20 publications

Lack of a role for natural killer cells in early control of Brucella abortus 2308 infections in mice

Lack of a role for natural killer cells in early control of Brucella abortus 2308 infections in mice

Cellular Responses to Bacterial Lipopolysaccharide: T Cells Recognize LPS Determinants

Mechanism of protective immunity induced by porin-lipopolysaccharide against murine salmonellosis

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