2012
DOI: 10.3892/ijmm.2012.957
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Differential effects of TGF-β1 and FGF-2 on SDF-1α expression in human periodontal ligament cells derived from deciduous teeth in vitro

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Cited by 9 publications
(9 citation statements)
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“…Indeed, the expression level was much higher than that in human dermal fibroblasts (17). Hasegawa et al reported that SDF-1α expression was increased in hPDLF by treatment with transforming growth factor-β1 but inhibited by treatment with basic fibroblast growth factor-2 (18), which suggests that fibroblasts exhibit cell type-specific reactivity to external stimuli. ANG, VEGF, and SDF-1α are potent angiogenic factors.…”
Section: Discussionmentioning
confidence: 98%
“…Indeed, the expression level was much higher than that in human dermal fibroblasts (17). Hasegawa et al reported that SDF-1α expression was increased in hPDLF by treatment with transforming growth factor-β1 but inhibited by treatment with basic fibroblast growth factor-2 (18), which suggests that fibroblasts exhibit cell type-specific reactivity to external stimuli. ANG, VEGF, and SDF-1α are potent angiogenic factors.…”
Section: Discussionmentioning
confidence: 98%
“…It has been demonstrated in previous studies that a number of cytokines and bioactive materials have clinical uses (32,33). One such cytokine molecule, FGF-2, is a multifunctional growth factor that exerts various effects, including the induction of proliferation and differentiation of a wide range of mesodermal and neuroectodermal cells (10,32,33). FGF-2 is also a crucial factor in wound healing and is involved in the induction of angiogenesis, cell proliferation and the accumulation and modulation of the extracellular matrix (34).…”
Section: Discussionmentioning
confidence: 99%
“…Transmembrane migration assay. Cell migration was evaluated using a Transwell system as previously described (10). Chambers with a 8-µm pore size and 6.4 mm in diameter were used (Corning Cell Culture Insert 24-well System; Sigma-Aldrich, St. Louis, MO, USA).…”
Section: Reverse Transcription Polymerase Chain Reaction (Rt-pcr)mentioning
confidence: 99%
“…RNA (1 µg) was reversetranscribed to first-strand cDNA using a PrimeScript™ RT reagent kit (Takara Bio, Inc., Shiga, Japan) according to the manufacturer's instructions. A Thermal Cycler Dice real-time system (Takara Bio, Inc.) was used for real-time PCR (18)(19)(20). The cDNAs were amplified with SYBR ® Premix Ex Taq and specific primer pairs for fibroblast growth factor (FGF)-1 (21), FGF-2 (22), epidermal growth factor (EGF) (23), vascular endothelial growth factor (VEGF) (24,25), insulin-like growth factor-1 (IGF-1) (26), nerve growth factor (NGF), neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), neuropeptide Y, β-tubulin Ⅲ, 2' ,3'-cyclic-nucleotide phosphodiesterase (CNPase), microtubule-associated protein 2 (Map2), glial fibrillary acidic protein (GFAP), nestin, glutamate receptor 1 (GluR1) (27), glutamate receptor 2 (GluR2) (27), glutamate receptor 3 (GluR3) (27), glutamate receptor 4 (GluR4) (27), N-methyl D-aspartate receptor subtype 2B (NR2B) (28) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH).…”
Section: Volume Measurement Of the Granular Cell Layer Of The Dgmentioning
confidence: 99%