Differential effects of the combination of tyrosol with chlorhexidine gluconate on oral biofilms

Vale, Luana Ribeiro do; Delbem, Alberto Carlos Botazzo; Arias, Laís Salomão; Fernandes, Rosa Áurea Quintella; Vieira, Ana Paula Miranda; Barbosa, Débora de Barros; Monteiro, Douglas Roberto

doi:10.1111/odi.12648

Cited by 18 publications

(10 citation statements)

References 25 publications

(35 reference statements)

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“…Arias et al (2016) showed that tyrosol treatment at concentrations ranging from 100 to 200 mM exerted a significant reduction in metabolic activity against C. albicans and C. glabrata two-day-old oral biofilms, which was proportional to a reduction in cell number [ 103 ]. Do Vale et al (2017) showed that tyrosol alone at concentrations of 50 and 90 mM demonstrated inhibition of the planktonic growth of C. albicans and C. glabrata cells, respectively [ 104 ]. However, tyrosol does not significantly reduce metabolic activity or the number of cells for one-day-old oral biofilms; in addition, the nature of interaction of tyrosol with chlorohexidine gluconate was indifferent.…”

Section: Tyrosolmentioning

confidence: 99%

“…However, tyrosol does not significantly reduce metabolic activity or the number of cells for one-day-old oral biofilms; in addition, the nature of interaction of tyrosol with chlorohexidine gluconate was indifferent. Nevertheless, 1.25 mM tyrosol with 0.00725 mM chlorhexidine gluconate showed a synergistic interaction in reducing the number of hyphae formed [ 104 ]. A combination of tyrosol and farnesol has been explored for oral Candida isolates for both planktonic and sessile growth.…”

Section: Tyrosolmentioning

confidence: 99%

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Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Kovács

Majoros

2020

JoF

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The number of effective therapeutic strategies against biofilms is limited; development of novel therapies is urgently needed to treat a variety of biofilm-associated infections. Quorum sensing is a special form of microbial cell-to-cell communication that is responsible for the release of numerous extracellular molecules, whose concentration is proportional with cell density. Candida-secreted quorum-sensing molecules (i.e., farnesol and tyrosol) have a pivotal role in morphogenesis, biofilm formation, and virulence. Farnesol can mediate the hyphae-to-yeast transition, while tyrosol has the opposite effect of inducing transition from the yeast to hyphal form. A number of questions regarding Candida quorum sensing remain to be addressed; nevertheless, the literature shows that farnesol and tyrosol possess remarkable antifungal and anti-biofilm effect at supraphysiological concentration. Furthermore, previous in vitro and in vivo data suggest that they may have a potent adjuvant effect in combination with certain traditional antifungal agents. This review discusses the most promising farnesol- and tyrosol-based in vitro and in vivo results, which may be a foundation for future development of novel therapeutic strategies to combat Candida biofilms.

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Section: Tyrosolmentioning

confidence: 99%

Section: Tyrosolmentioning

confidence: 99%

Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Kovács

Majoros

2020

JoF

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“…Because implants need a rough surface for osseointegration, the use of mechanical measures, such as periodontal scaling [10], dental floss, or rinsing may not be a feasible option [11]. Chemical plaque control, such as the use of chlorhexidine mouthwashes, has an effective role in controlling microorganisms including Streptococcus mutans [12] and Candida albicans [13] and periodontal pathogens such as P. gingivalis and Aggregatibacter actinomycetemcomitans [14]. Although chlorhexidine has an effective action against oral bacteria, its long-term use can lead to unwanted effects, such as altered sensations in taste, discoloration of the oral mucosa/tongue/gingiva [15], imbalances of the oral flora, and the development of drug resistance [16].…”

Section: Introductionmentioning

confidence: 99%

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Liu

et al. 2020

J Oral Sci

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Porphyromonas gingivalis (P. gingivalis) is one of the major pathogenic bacteria of periodontitis or peri-implantitis. P. gingivalis tends to attach to the implant's neck with the formation of biofilm, leading to peri-implantitis. d-arginine has been shown to have a potential antimicrobial role. In this study, P. gingivalis was cultured in Brain Heart Infusion broth together with d-arginine. After 3 days (inhibition) or 6 days (dissociation), these were characterized using crystal violet (CV) staining for the biofilm, extracellular polysaccharide (EPS) production from the biofilm, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for biofilm activation. Furthermore, the P. gingivalis biofilm was observed by scanning electron microscopy (SEM). d-arginine effectively reduced biomass accumulation and promoted dissociation at concentrations of ≥50 mM and 100 mM, respectively. Through CV staining, d-arginine concentrations of EPS production from the biofilm for inhibition and dissociation effects was ≥50 mM and 100 mM, respectively. In addition, d-arginine affected biofilm activation for the corresponding concentrations: ≥60 mM for inhibition and ≥90 mM for dispersal. Under SEM observation, d-arginine changed the P. gingivalis biofilm structure in relatively high concentrations for inhibition or dissociation, respectively. The authors concluded that d-arginine could inhibit the formation of P. gingivalis biofilm and promote the dissociation of P. gingivalis biofilm.

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“…Chlorhexidine at low concentrations, but with the addition of cis-2-decenoic acid, was able to disperse single-species biofilms formed by S. mutans and C. albicans, as well as bacterial-fungal dual-species consortia [56]. Also, chlorhexidine gluconate with tyrosol was revealed to be effective against single and mixed-species oral biofilms [57,58]. A solution containing 2.5% sodium hypochlorite, 2% chlorhexidine, and ozonated water inhibited biofilms of S. mutans and C. albicans in mesiobuccal root canals after irrigation [25].…”

Section: Chemical Compounds With Activity Against Streptococcus Mutanmentioning

confidence: 99%

Management of Streptococcus mutans-Candida spp. Oral Biofilms’ Infections: Paving the Way for Effective Clinical Interventions

Salehi

Kręgiel

Mahady

et al. 2020

JCM

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Oral diseases are considered the most common noncommunicable diseases and are related to serious local and systemic disorders. Oral pathogens can grow and spread in the oral mucosae and frequently in biomaterials (e.g., dentures or prostheses) under polymicrobial biofilms, leading to several disorders such as dental caries and periodontal disease. Biofilms harbor a complex array of interacting microbes, increasingly unapproachable to antimicrobials and with dynamic processes key to disease pathogenicity, which partially explain the gradual loss of response towards conventional therapeutic regimens. New drugs (synthesized and natural) and other therapies that have revealed promising results for the treatment or control of these mixed biofilms are presented and discussed here. A structured search of bibliographic databases was applied to include recent research. There are several promising new approaches in the treatment of Candida spp.–Streptococcus mutans oral mixed biofilms that could be clinically applied in the near future. These findings confirm the importance of developing effective therapies for oral Candida–bacterial infections.

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Differential effects of the combination of tyrosol with chlorhexidine gluconate on oral biofilms

Abstract: The combination of tyrosol with chlorhexidine gluconate was only effective in reducing the number of hyphae by C. albicans, a relevant virulence factor of this species.

Cited by 18 publications

References 25 publications

Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Fungal Quorum-Sensing Molecules: A Review of Their Antifungal Effect against Candida Biofilms

Effects of D-arginine on <i>Porphyromonas gingivalis</i> biofilm

Management of Streptococcus mutans-Candida spp. Oral Biofilms’ Infections: Paving the Way for Effective Clinical Interventions

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