“…Consistent with this data, genistein-bound ERα recruits the steroid receptor coactivator (SRC)-3 nuclear receptor (NR) box I of the p160 transcriptional coactivator family with less than half of the efficacy of E2-bound ERα, and SRC-3 NR box II is not recruited at all (Routledge et al, 2000;Bramlett et al, 2001). On the other hand, the crystal structure of ERβ with genistein indicates that H12 is positioned on the surface of the LBD and the binding of SRC-3 NR box I showed a 3-fold amount in the presence of genistein greater than E2-bound receptor (Routledge et al, 2000;Bramlett et al, 2001). Thus, selective coactivator recruitment may be an underlying mechanism for tissue/cell (anti) estrogenicity of genistein.…”