Differential efficacy of endodontic obturation procedures: an ex vivo study

Abstract

By means of a double-chamber model, different root canal filling materials and procedures were compared. Briefly, the root canals of single-rooted human teeth, extracted for periodontal reasons, were instrumented and obturated by gutta-percha/Pulp Canal Sealer EWT (PCS) or by Resilon, in association with different sealers (Real Seal, RelyX Unicem or Meta). Obturation was achieved by traditional continuous wave of condensation technique (TCWCT), a modified version of it (MCWCT), or single cone technique (SCT). The obturated roots, inserted in a double-chamber model, were sterilized by gamma irradiation. Next, Enterococcus faecalis was added to the upper chamber and the specimens were incubated at 37 °C for up to 120 days; the development of turbidity in the lower chambers' broths indicated bacterial leakage through the obturated root canals. The kinetics of leakage were analyzed in different groups by means of Kaplan-Meier statistics and compared by log-rank test. The results showed that root canals obturated with either gutta-percha/PCS using the MCWCT, Resilon/Real Seal SCT or Resilon/RelyX Unicem using the TCWCT displayed significantly better performance than the remaining groups (p< 0.01). Histological evaluation, performed to investigate microbial localization inside specimens, confirmed that this parameter varied according to the obturation procedures and materials employed. This ex vivo study indicates that gutta-percha/PCS, if used with the MCWCT, is as effective as Resilon when coupled to Real Seal with the SCT or, interestingly, to RelyX Unicem with the TCWCT. These data suggest that further improvement of the currently employed root canal filling procedures is achievable, depending on both the filling materials and the technique employed, thus encouraging clinical studies in this direction.

Sodium hypochlorite solution penetration into human dentine: a histochemical evaluation

“…); otherwise, infiltration of tissue fluids could provide a substrate for bacterial growth (Siqueira , Ardizzoni et al . ). A possible way of improving the bactericidal efficacy of disinfecting solutions is to incorporate detergents as surface‐active agents to enhance their wetting properties (Glantz & Hansson , Giardino et al .…”

“…Increasing the concentration of NaOCl from 1% to 6% improved the penetration depth of its antibacterial action, but complete eradication of bacteria from dentinal tubules could not be achieved, especially beyond the first 300 lm from the pulp-dentine junction (Wong & Cheung 2014). Therefore, remaining bacteria should be entombed by root fillings (Peters et al 1995); otherwise, infiltration of tissue fluids could provide a substrate for bacterial growth (Siqueira 2001, Ardizzoni et al 2014. A possible way of improving the bactericidal efficacy of disinfecting solutions is to incorporate detergents as surface-active agents to enhance their wetting properties (Glantz & Hansson 1972.…”

Double dye technique and fluid filtration test to evaluate early sealing ability of an endodontic sealer

“…The tridimensional root filling could prevent coronal infection or re-infection [2,3]. However, root canal fillings are affected by gaps that alter their sealing ability [4]. Gaps may be connected and may create a sort of complex spiral network that affects the tridimensional morphology of the root filling [5].…”

Push-out bond strength of a self-adhesive resin cement used as endodontic sealer

“…Several in vitro studies have reported bond strength values of different adhesive systems used in combination with a luting composite to both enamel and dentin 16,17,18,19,20. In view of these desirable features, this material could be used as an endodontic sealer.…”

“…In view of these desirable features, this material could be used as an endodontic sealer. A recent study demonstrated good results when associating RelyX Unicem to Resilon cones 20. However, up to the present date, little information regarding its potential to be used as an endodontic sealer is available in the literature.…”