Differential expansion and evolution of the exon family encoding the Serpin-1 reactive centre loop has resulted in divergent serpin repertoires among the Lepidoptera

Hegedus, Dwayne D.; Erlandson, Martin A.; Baldwin, Doug; Hou, Xingwei; Chamankhah, Mahmood

doi:10.1016/j.gene.2008.03.015

Cited by 24 publications

(16 citation statements)

References 28 publications

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“…5). This expression profi le of Apserpin-10 is consistent with that of M. confi gurata serpin-1d (Hegedus et al, 2008), Choristoneura fumiferana serpin-1 (Zheng et al, 2009) andA. pernyi serpin-3 and14 (Kausar et al, 2017;Wang et al, 2017).…”

Section: Discussionsupporting

confidence: 74%

Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

Kausar¹,

Qian²,

Abbas³

et al. 2017

Eur. J. Entomol.

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Section: Discussionsupporting

confidence: 74%

Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

Kausar¹,

Qian²,

Abbas³

et al. 2017

Eur. J. Entomol.

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“…Apart from gene duplication, diversity of serpins can also be generated through alternative exon splicing [45, 46]. This mechanism, first discovered in M. sexta [47], represents a parsimonious way of producing variations in RSL sequence and inhibitory selectivity.…”

Section: Discussionmentioning

confidence: 99%

A comparative analysis of serpin genes in the silkworm genome

et al. 2009

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Serine protease inhibitors (serpins) are a superfamily of proteins, most of which control protease-mediated processes by inhibiting their cognate enzymes. Sequencing of the silkworm genome provides an opportunity to investigate serpin structure, function, and evolution at the genome level. There are thirty-four serpin genes in Bombyx mori. Six are highly similar to their Manduca sexta orthologs that regulate innate immunity. Three alternative exons in serpin1 gene and four in serpin28 encode a variable region including the reactive site loop. Splicing of serpin2 pre-mRNA yields variations in serpin2A, 2A′ and 2B. Sequence similarity and intron positions reveal the evolutionary pathway of seven serpin genes in group C. RT-PCR indicates an increase in the mRNA levels of serpin1, 3, 5, 6, 9, 12, 13, 25, 27, 32 and 34 in fat body and hemocytes of larvae injected with bacteria. These results suggest that the silkworm serpins play regulatory roles in defense responses.

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“…Orthologous serpin-1 genes from other lepidopteran species, with alternate exons in the same position as in M. sexta serpin-1, have been identified [23–25]. The serpin-1 gene of B. mori , in the same superfamily as M. sexta , has only four alternate versions of exon 9 [11,26], indicating considerable genetic flexibility and relatively recent expansions and divergence of these alternate exons in lepidopteran evolution [24]. Alternative splicing at the same position, to produce serpins with differing RCLs also occurs in An.…”

Section: Introductionmentioning

confidence: 99%

Serpins in arthropod biology

Meekins

Kanost

Michel

2017

Seminars in Cell & Developmental Biology

150

136

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Serpins are the largest known family of serine proteinase inhibitors and perform a variety of physiological functions in arthropods. Herein, we review the field of serpins in arthropod biology, providing an overview of current knowledge and topics of interest. Serpins regulate insect innate immunity via inhibition of serine proteinase cascades that initiate immune responses such as melanization and antimicrobial peptide production. In addition, several serpins with anti-pathogen activity are expressed as acute-phase serpins in insects upon infection. Parasitoid wasps can downregulate host serpin expression to modulate the host immune system. In addition, examples of serpin activity in development and reproduction in Drosophila have also been discovered. Serpins also function in host-pathogen interactions beyond immunity as constituents of venom in parasitoid wasps and saliva of blood-feeding ticks and mosquitoes. These serpins have distinct effects on immunosuppression and anticoagulation and are of interest for vaccine development. Lastly, the known structures of arthropod serpins are discussed, which represent the serpin inhibitory mechanism and provide a detailed overview of the process.

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Differential expansion and evolution of the exon family encoding the Serpin-1 reactive centre loop has resulted in divergent serpin repertoires among the Lepidoptera

Cited by 24 publications

References 28 publications

Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

Characterization and functional analysis of the serpin-10 gene from oak silkworm, Antheraea pernyi (Lepidoptera: Saturniidae)

A comparative analysis of serpin genes in the silkworm genome

Serpins in arthropod biology

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