Differential expression and anti‐oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization

Xu, Xiu; Leng, Jing-Yu; Gao, Fei; Zhao, Zhen-Ao; Deng, Wenbo; Liang, Xiao-Huan; Zhang, Yi-Juan; Zhang, Zhirong; Li, Ming; Sha, Aihua; Yang, Zeng‐Ming

doi:10.1016/j.febslet.2014.02.043

Cited by 34 publications

(32 citation statements)

References 43 publications

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“…В 2014 г. в FEBS Letters была опубликована работа [23], выполненная главным образом на мышах, указывающая на тесную связь экс-прессии GPX3 в стромальных клетках эндоме-трия с децидуализацией эндометрия и имплан-тацией бластоцисты в стенку матки. Результаты этой работы объясняют механизмы, по кото-рым происходит регуляция экспрессии GPX3 в эндометрии.…”

Section: исследования экспрессии глутатионпероксидаз в ткани эндометрunclassified

“…Даже в конце 5-го дня нормальной беременности имплантация может все еще не произойти [24]. Но, вероятно, мыши, используемые в работе [23], были очень сходны между собой по времени имплантации…”

Section: исследования экспрессии глутатионпероксидаз в ткани эндометрunclassified

“…Выявленное авторами увеличение экспрессии мРНК Gpx1 на 8-й день беременности, возможно, не являет-ся статистически значимым, поскольку из работы не ясно, каким методом пользовались авторы для статистических сравнений, а прирост экспрессии по сравнению с 4-м и 5-м днями сравнительно мал. Экспрессия мРНК Gpx5, Gpx6 и Gpx7 в эндо-метрии была очень слабой в исследуемый период (1-8-й дни беременности) [23]. При задержке имплантации с последующей ее активацией эстрогеном опять-таки выявле-но увеличение экспрессии мРНК Gpx3 методом гибридизации in situ до хорошо детектируемого уровня в строме, окружающей имплантирую-щийся эмбрион.…”

Section: исследования экспрессии глутатионпероксидаз в ткани эндометрunclassified

“…При искусственной дециду-ализации посредством инъекции масла в рог матки также выявлен высокий уровень экспрес-сии мРНК Gpx3 в клетках стромы. В роге матки, который не подвергался инъекции, экспрессия мРНК Gpx3 регистрировалась в поверхност-ном эпителии эндометрия и в меньшей степени в железистом эпителии, но не в клетках стро-мы [23].…”

Section: исследования экспрессии глутатионпероксидаз в ткани эндометрunclassified

“…В опытах in vitro установлена необходимость добавления соединений селена (селенит на-трия -в концентрациях порядка 0,1 мкМ) в культуральную среду с клетками эндометрия для экспрессии в них белка GPX3, что понятно, поскольку селен включается в состав молекулы фермента при трансляции (без него белковая молекула не синтезируется [25]). Получены данные, свидетельствующие в пользу того, что GPX3 снижает концентрацию пероксида водо-рода в строме [23].…”

Section: исследования экспрессии глутатионпероксидаз в ткани эндометрunclassified

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Significance of glutathione peroxidases in endometrium function: facts, hypotheses, and research perspectives

2017

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Enzymes of glutathione peroxidase (GPX) family, together with peroxiredoxins, form thiol peroxidase superfamily, the property of which is the thiol-dependent catalysis of the hydroperoxide reduction. This property determines them as antioxidant protectors. Among human GPXs the eight forms are known, five of which are selenium-dependent (GPX1,2,3,4, and 6). The most number of facts supporting the substantial role of GPX in functioning of endometrium are linked with the GPX3, which is the secretory GPX. The number of candidate progesterone response elements in the promoter of GPX3 gene prevails over the number of candidate estrogen response elements; GPX3 is upregulated gene during postovulatory phase of reproductive cycle and during pregnancy; in the endometrial stroma, the transcription of Gpx3 is stimulated through the transcription factor HIF1α. Using laboratory animals, the spatial and temporal coincidence of Gpx3 activation and blastocyst implantation was observed. It was confirmed that GPX3 decreases hydrogen peroxide concentration in endometrium in pregnant animals and during in vitro decidualization. The vulnerability of reproductive function to physiological stress at the insufficient expression of GPX3 is hypothesized. The GPX3 enzymatic activity in endometrium is poorly investigated. The has been hypothesized that selenium-containing medications are effective in the endometrium receptivity improvement and in the supporting the normal embryo development (especially during the influence of physiological stressors) by the maintenance of the posttranscriptional, selenium-dependent stage of GPX3 biosynthesis in endometrium. Probably, the GPX3 expression can be increased by the steriods with gestagenic activity (through the increase of Gpx3 transcription, analogously to effect of progesterone). In contrast with the «classic» GPX (GPX1) and probably with most of other members of GPX family, GPX3 has a wide thiol specificity. It is proposed to assess the activity of GPX3 using the reduced homocysteine and cysteine as thiol substrates instead of the reduced glutathione.

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Section: исследования экспрессии глутатионпероксидаз в ткани эндометрunclassified

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Significance of glutathione peroxidases in endometrium function: facts, hypotheses, and research perspectives

2017

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Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer

Rabajdová,

Špaková,

Smolko

et al. 2023

FEBS Open Bio

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Abstract:Endometriosis and endometrial cancer are closely related with oxidative stress. However, the direct relationship between copper and zinc levels and oxidative stress in the extracellular and intracellular space remains unclear. The presented study is focused on determination of serum Zn and Cu levels, glutathione concentration and enzyme activity in three groups: patients diagnosed with endometrial cancer (EC), patients diagnosed with endometriosis (EM) and a healthy control group. Spectrophotometric determination of trace elements revealed that levels of zinc and copper were lower in blood plasma of patients with endometriosis as compared with the other groups; however, there were no significant differences in the Cu/Zn ratio. Furthermore, significantly increased blood serum glutathione levels were detected in both EM and EC groups compared to the control group. While the activity of superoxide dismutase (SOD) was similar across the studied groups, we observed differences in the activity of other enzymes associated with oxidative stress, including glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S‐transferase (GST), between the control group and the EM and EC patients. Additionally, analysis of gene expression based on free circulating mRNA indicated significant differences in the expression of SOD isoenzymes between the patient groups and the control group; expression of GPx isoenzymes was also altered. Our results may have potential application in diagnostics as well as monitoring of endometriosis and endometrial cancer.

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Selenium in Food Chain in Relation to Human and Animal Nutrition and Health

Kurt

Özdemir

2022

Sustainable Plant Nutrition in a Changing World

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Differential expression and anti‐oxidant function of glutathione peroxidase 3 in mouse uterus during decidualization

Cited by 34 publications

References 43 publications

Significance of glutathione peroxidases in endometrium function: facts, hypotheses, and research perspectives

Significance of glutathione peroxidases in endometrium function: facts, hypotheses, and research perspectives

Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer

Selenium in Food Chain in Relation to Human and Animal Nutrition and Health

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