Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis

Venditti, Massimo; Minucci, Sergio

doi:10.3390/biomedicines10010181

Cited by 8 publications

(4 citation statements)

References 39 publications

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“…For IF staining, testis sections were processed according to Venditti and Minucci (2022) . For details on the used antibodies, see Supplementary Table S2 .…”

Section: Methodsmentioning

confidence: 99%

The simultaneous administration of microplastics and cadmium alters rat testicular activity and changes the expression of PTMA, DAAM1 and PREP

Venditti

Hassine

Messaoudi

et al. 2023

Front. Cell Dev. Biol.

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This paper confirms the damaging effects produced by MP and Cd on testicular activity in the rat. Oral treatment with both chemicals resulted in testicular damage, documented by biomolecular and histological alterations, particularly by impaired morphometric parameters, increased apoptosis, reduced testosterone synthesis, and downregulation of the steroidogenic enzyme 3β-HSD. We also demonstrated, for the first time, that both MP and Cd can affect the protein level of PTMA, a small peptide that regulates germ cell proliferation and differentiation. Interestingly, the cytoarchitecture of testicular cells was also altered by the treatments, as evidenced by the impaired expression and localization of DAAM1 and PREP, two proteins involved in actin- and microtubule-associated processes, respectively, during germ cells differentiation into spermatozoa, impairing normal spermatogenesis. Finally, we showed that the effect of simultaneous treatment with MP and Cd were more severe than those produced by MP alone and less harmful than those of Cd alone. This could be due to the different ways of exposure of the two substances to rats (in drinking water for Cd and in oral gavage for MP), since being the first contact in the animals’ gastrointestinal tract, MP can adsorb Cd, reducing its bioavailability through the Trojan-horse effect.

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“…For IF staining, testis sections were processed according to Venditti and Minucci (2022) . For details on the used antibodies, see Supplementary Table S2 .…”

Section: Methodsmentioning

confidence: 99%

The simultaneous administration of microplastics and cadmium alters rat testicular activity and changes the expression of PTMA, DAAM1 and PREP

Venditti

Hassine

Messaoudi

et al. 2023

Front. Cell Dev. Biol.

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“…The Golgi apparatus is responsible for acrosome formation during spermatogenesis ( Khawar, Gao & Li, 2019 ). In the early stages of spermatid development, the Golgi-derived small vesicles gather in the concave area of the sperm nucleus, and along with sperm development, the small vesicles fuse to form large acrosome granules ( Venditti & Minucci, 2022 ). PICK1 and Gopc gene are essential for acrosome formation in male germ cells ( Bizkarguenaga et al, 2019 ; Du et al, 2023 ).…”

Section: Discussionmentioning

confidence: 99%

GGA1 participates in spermatogenesis in mice under stress

Jiao

Chen

Han

et al. 2023

PeerJ

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Background Infertility is recognized as a common and worrisome problem of human reproduction worldwide. Based on previous studies, male factors account for about half of all infertility cases. Exposure to environmental toxicants is an important contributor to male infertility. Bisphenol A (BPA) is the most prominent toxic environmental contaminant worldwide affecting the male reproductive system. BPA can impair the function of the Golgi apparatus which is important in spermatogenesis. GGA1 is known as Golgi-localized, gamma adaptin ear-containing, ARF-binding protein 1. Previously, it has been shown that GGA1 is associated with spermatogenesis in Drosophila, however, its function in mammalian spermatogenesis remains unclear. Methods Gga1 knockout mice were generated using the CRISPR/Cas9 system. Gga1-/- male mice and wild-type littermates received intraperitoneal (i.p.) injections of BPA (40 µg/kg) once daily for 2 weeks. Histological and immunofluorescence staining were performed to analyze the phenotypes of these mice. Results Male mice lacking Gga1 had normal fertility without any obvious defects in spermatogenesis, sperm count and sperm morphology. Gga1 ablation led to infertility in male mice exposed to BPA, along with a significant reduction in sperm count, sperm motility and the percentage of normal sperm. Histological analysis of the seminiferous epithelium showed that spermatogenesis was severely disorganized, while apoptotic germ cells were significantly increased in the Gga1 null mice exposed to BPA. Our findings suggest that Gga1 protects spermatogenesis against damage induced by environmental pollutants.

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“…For LC3, 3β‐HSD, OCN, and CX43 staining, 5 μm testis sections were deparaffinized, rehydrated, and processed as described previously by (Venditti & Minucci, 2022). Sections were incubated overnight at 4°C with primary antibodies, diluted 1:100.…”

Section: Methodsmentioning

confidence: 99%

Melatonin counteracts cadmium‐induced rat testicular toxicity via the mechanistic target rapamycin (mTOR) pathway

Rhouma,

Venditti,

Haddadi

et al. 2024

J Exp Zool Pt A

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The protective action of melatonin (MLT) against the harmful effects of cadmium (Cd) on testicular activity in rats has been documented previously; however, the involved molecular mechanisms have yet to be elucidated. Herein, we investigate the involvement of the mammalian target of rapamycin (mTOR) on the ability of MLT to counteract the damage induced by Cd on the rat testicular activity. Our study confirmed that Cd has harmful effects on the testes of rats and the protective action exerted by MLT. We reported, for the first time, that the addition of rapamycin (Rapa), a specific mTOR inhibitor, to animals co‐treated with Cd and MLT completely abolished the beneficial effects exerted by MLT, indicating that the mTOR pathway partially modulates its helpful effects on Cd testicular toxicity. Interestingly, Rapa‐alone treatment, provoking mTOR inhibition, produced altered morphological parameters, increased autophagy of germ and somatic cells, and reduced serum testosterone concentration. In addition, mTOR inhibition also reduced protein levels of markers of steroidogenesis (3β‐Hydroxysteroid dehydrogenase) and blood–testis barrier integrity (occludin and connexin 43). Finally, Rapa altered sperm parameters as well as the ability of mature spermatozoa to perform a proper acrosome reaction. Although further investigation is needed to better clarify the molecular pathway involved in MLT action, we confirm that MLT alleviating Cd effects can be used as a supplement to enhance testicular function and improve male gamete quality.

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Differential Expression and Localization of EHBP1L1 during the First Wave of Rat Spermatogenesis Suggest Its Involvement in Acrosome Biogenesis

Cited by 8 publications

References 39 publications

The simultaneous administration of microplastics and cadmium alters rat testicular activity and changes the expression of PTMA, DAAM1 and PREP

The simultaneous administration of microplastics and cadmium alters rat testicular activity and changes the expression of PTMA, DAAM1 and PREP

GGA1 participates in spermatogenesis in mice under stress

Melatonin counteracts cadmium‐induced rat testicular toxicity via the mechanistic target rapamycin (mTOR) pathway

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