Differential expression and roles of volume-activated chloride channels in control of growth of normal and cancerous nasopharyngeal epithelial cells

ClC-3 is a candidate of the channel proteins mediating acid-activated chloride currents in nasopharyngeal carcinoma cells

“…Cell shrinkage induced by hypertonic challenges inhibited the currents. Furthermore, both the acid-induced Cl Ϫ currents and the volume-activated Cl Ϫ currents were attenuated by ClC-3 siRNA treatments (31). These results suggest that the acidinduced chloride channel is a volume-sensitive chloride channel.…”

“…ClC-3 was expressed in CNE-2Z cells and has been proposed by others and us to be the molecular candidate involved in the activation or regulation of volume-sensitive Cl Ϫ currents (7,9,18,28,31). To investigate the role of ClC-3 in the activation of the acid-activated chloride currents, the effect of knockdown of ClC-3 expression by ClC-3 siRNA was tested.…”

Changing expression of chloride channels during preimplantation mouse development

“…However, the molecular identity of VSOAC has not been firmly established to be CLCN3. There is also evidence in other cell types for the involvement of CLCN3 in Cl K currents observed under isotonic conditions (Yang et al 2011) and for these currents being required for cell proliferation (Habela et al 2008, Tang et al 2008, Zhu et al 2012. In mouse embryos, VSOAC is only present in the oocyte, 1-, and 2-cell stages in which its activation by hypotonicity is cell cycle dependent (Kolajova & Baltz 1999, Kolajova et al 2001.…”

The AQP-3 water channel and the ClC-3 chloride channel coordinate the hypotonicity-induced swelling volume in nasopharyngeal carcinoma cells

“…We and others have shown that ClC-3, a candidate of volumesensitive Cl − channels, is the main chloride channel protein mediating the cell volume changes associated with proliferation, migration and apoptosis (Chen et al, 2002;Mao et al, 2008Mao et al, , 2009Mao et al, , 2012Mao et al, , 2013Zhang et al, 2013a;Zhang et al, 2013b;Zhu et al, 2012). We demonstrated previously that lithium could inhibit the swelling-induced Cl − current (I Cl,swell ) in nasopharyngeal carcinoma cells (Bai et al, 2010).…”