Differential expression and translocation of protein tyrosine phosphatase 1B-related proteins in ME-180 tumor cells expressing apoptotic sensitivity and resistance to tumor necrosis factor: potential interaction with epidermal growth factor receptor

Perez, Margot; Haschke, Brian; Donato, Nicholas J.

doi:10.1038/sj.onc.1202368

Cited by 15 publications

(21 citation statements)

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“…Another mechanism resulting in an increased EGFR expression and connected with the stimulation of TNF· receptor has also been noted (32,(34)(35)(36). Evidence suggested that the overexpression of tyrosine kinase activity EGFR may suppress the antiproliferative or cytotoxic activity of TNF· (32,36). Interestingly, EGFR expression can be increased by TNF· via the p55 receptor (36).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, decreased EGFR tyrosine phosphorylation leads to the blockade of the EGFR-mediated signal transduction (32). Another mechanism resulting in an increased EGFR expression and connected with the stimulation of TNF· receptor has also been noted (32,(34)(35)(36). Evidence suggested that the overexpression of tyrosine kinase activity EGFR may suppress the antiproliferative or cytotoxic activity of TNF· (32,36).…”

Section: Introductionmentioning

confidence: 99%

“…Evidence for the stimulation of protein tyrosine phosphatase and the reduction of EGFR tyrosine phosphorylation by TNF· has been provided. Thus, decreased EGFR tyrosine phosphorylation leads to the blockade of the EGFR-mediated signal transduction (32). Another mechanism resulting in an increased EGFR expression and connected with the stimulation of TNF· receptor has also been noted (32,(34)(35)(36).…”

Section: Introductionmentioning

confidence: 99%

“…In squamous cell head and neck carcinomas (HNSCC), constitutively active STAT3 has been shown to be associated with EGFR signaling and deregulated cell growth (20,21,27,28). Nevertheless, several studies have been reported, which demonstrate the interaction of proinflammatory cytokines with EGFR as well as the effects of antitumor cytokine connected with the activation of cell death program (29)(30)(31)(32)(33). It is known that TNF· is capable of activating tumor cell apoptosis through receptor clustering and the stimulation of caspases (caspase-8) (31,32).…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, several studies have been reported, which demonstrate the interaction of proinflammatory cytokines with EGFR as well as the effects of antitumor cytokine connected with the activation of cell death program (29)(30)(31)(32)(33). It is known that TNF· is capable of activating tumor cell apoptosis through receptor clustering and the stimulation of caspases (caspase-8) (31,32). Evidence for the stimulation of protein tyrosine phosphatase and the reduction of EGFR tyrosine phosphorylation by TNF· has been provided.…”

Section: Introductionmentioning

confidence: 99%

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Impact of EGFR immunoexpression on STAT3 activation and association with proinflammatory/regulatory cytokine pattern in laryngeal squamous cell carcinoma

Starska

Bryś²,

Forma³

et al. 2009

Oncol Rep

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Abstract. Stimulation of epidermal growth factor receptor (EGFR) results in the activation of signal transducer and activator of transcription-3 (STAT3), a transcriptional factor associated with carcinogenesis. Proinflammatory cytokines are capable of activating a tumor cell death program by reducing EGFR tyrosine phosphorylation. This study aimed to identify EGFR expression in laryngeal carcinoma and determine the relationship with STAT3 and proinflammatory/ regulatory cytokine secretion. An analysis of EGFR expression (membranous EGFR-m and cytoplasmic EGFR-c) was performed in tumor tissues by immunohistochemical (IHC) staining in 45 medical cases of laryngeal carcinoma. STAT3 expression in freshly isolated tumor and non-cancerous normal epithelial cells by RT-PCR was analyzed in 24 patients after total larynx resection. The concentrations of TNF·, IL-2, IL-6, IL-8 and IFNÁ secreted by purified peripheral blood mononuclear cells (PBMCs) or contained in whole blood samples were measured by ELISA. The relationship between EGFR and mRNA STAT3 expression as well as the level of secreted cytokines was investigated. In our study, 93.3% tumors expressed EGFR-m and 37.8% EGFR-c. It also revealed a statistically significant dependence of the EGFR status on STAT3 expression in neoplastic tissues. Tumors with IHC EGFR-m positive staining >50% of the total number of cells, as well as with EGFR-c positive staining, were characterized by the most frequent presence of STAT3 expression. Our data demonstrate a significant negative relationship between EGFR-m expression and TNF· concentration, and a positive connection between membranous EGFR and IL-8 or IFNÁ levels recorded in isolated PBMCs. Furthermore, this study revealed a significant relationship between EGFR-c immunoexpression and IL-8 or IFNÁ concentration. Our findings have confirmed a key role of EGFR in determining the proliferative and malignant potential of laryngeal carcinoma.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Impact of EGFR immunoexpression on STAT3 activation and association with proinflammatory/regulatory cytokine pattern in laryngeal squamous cell carcinoma

Starska

Bryś²,

Forma³

et al. 2009

Oncol Rep

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Levels of protein tyrosine phosphatase 1B determine susceptibility to apoptosis in serum‐deprived hepatocytes

González-Rodriguez

Escribano

Alba

et al. 2007

Journal Cellular Physiology

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Protein tyrosine phosphatase 1B (PTP1B) is a negative regulator of tyrosine kinase growth factor signaling. To assess the importance of PTP1B in the balance between death and survival in the liver, we have developed immortalized neonatal hepatocyte cell lines lacking (PTP1B−/−) or overexpressing (PTP1B+/+PTP1B) PTP1B. Early activation of caspase‐3 occurred in PTP1B+/+PTP1B hepatocytes but was nearly abolished in PTP1B−/− cells. At the molecular level, PTP1B overexpression/deficiency altered the balance of pro‐(Bim) and anti‐(Bcl‐xL) apoptotic members of the Bcl‐2 family upon serum withdrawal. Likewise, cytosolic cytochrome C increased rapidly in PTP1B+/+PTP1B hepatocytes whereas it was retained in the mitochondria of PTP1B−/− cells. DNA fragmentation and the increase of apoptotic cells induced by serum withdrawal in wild‐type (PTP1B+/+) hepatocytes were absent in PTP1B−/− cells. Conversely, overexpression of PTP1B accelerated DNA laddering and increased the number of apoptotic cells. In serum‐deprived PTP1B+/+PTP1B hepatocytes, a rapid entry of Foxo1 into the nucleus and an earlier activation of caspase‐8 was observed. However, both events were suppressed in PTP1B−/− hepatocytes. Moreover, PTP1B deficiency conferred resistance to apoptosis induced by activation of Fas and constitutively active Foxo1. Rescue of PTP 1B in deficient hepatocytes recovered the phenotype of wild‐type cells whereas reduction of PTP1B by siRNA suppressed apoptosis. Our results reveal a unique role for PTP1B as a mediator of the apoptotic pathways triggered by trophic factors withdrawal in hepatocytes. This novel mechanism may represent an important target in the design of therapeutic strategies for human liver regeneration after pathological damage as well as for treatment of hepatocarcinomas. J. Cell. Physiol. 212: 76–88, 2007. © 2007 Wiley‐Liss, Inc.

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Role of Protein Tyrosine Phosphatase 1B in Hepatocyte-Specific Insulin and Growth Factor Signaling

González-Rodrı́guez

Valverde

2013

Protein Tyrosine Phosphatase Control of Metabolism

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Differential expression and translocation of protein tyrosine phosphatase 1B-related proteins in ME-180 tumor cells expressing apoptotic sensitivity and resistance to tumor necrosis factor: potential interaction with epidermal growth factor receptor

Cited by 15 publications

References 70 publications

Impact of EGFR immunoexpression on STAT3 activation and association with proinflammatory/regulatory cytokine pattern in laryngeal squamous cell carcinoma

Impact of EGFR immunoexpression on STAT3 activation and association with proinflammatory/regulatory cytokine pattern in laryngeal squamous cell carcinoma

Levels of protein tyrosine phosphatase 1B determine susceptibility to apoptosis in serum‐deprived hepatocytes

Role of Protein Tyrosine Phosphatase 1B in Hepatocyte-Specific Insulin and Growth Factor Signaling

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