Differential expression in glioblastoma multiforme and cerebral hemangioblastoma of cytoplasmic proteins that bind two different domains within the 3'-untranslated region of the human glucose transporter 1 (GLUT1) messenger RNA.

Tsukamoto, Haruhisa; Boado, Rubén J.; Pardridge, William M.

doi:10.1172/jci118738

Cited by 38 publications

(31 citation statements)

References 33 publications

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“…These observations suggest there is post-transcriptional regulation of BSAT1 gene expression, which produces low transporter in cells despite high mRNA levels. Such translational repression would be mediated by the binding of cellular proteins to specific sequences within the 5 0 -or 3 0 -UTR of the BSAT1 mRNA, similar to that previously showed for other BBB transporters, including the GLUT1 glucose transporter mRNA (Tsukamoto et al, 1996), or the LAT1 amino acid transporter mRNA (Boado et al, 2003b). The selective binding of brain endothelial polysome proteins to the full-length BSAT1 cloned RNA is showed in Figure 6.…”

Section: Discussionsupporting

confidence: 61%

Blood—Brain Barrier Genomics and Cloning of a Novel Organic Anion Transporter

Cai¹,

Li²,

Boado³

et al. 2007

J Cereb Blood Flow Metab

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A novel organic anion transporter selectively expressed at the blood-brain barrier (BBB), originally designated BBB-specific anion transporter type 1 (BSAT1), and now classified as Slco1c1, has been cloned from a BBB genomics program as a partial cDNA; this study describes the cloning and expression of the full-length cDNA from a rat brain capillary cDNA library. Northern analysis revealed the selective expression of the transporter at the BBB, and the transporter was expressed after permanent transfection of human 293 cells with cDNA encoding either the full length or open reading frame mRNA. The full-length transporter cDNA was 2.6 kb, and the mRNA was highly expressed at the rat brain microvasculature, but not in kidney, liver, heart, or lung, or in glial cells or brain glial tumors. Blood-brain barrier-specific anion transporter type 1 expression in 293 cells was poor after the transfection of the full-length cDNA, whereas transporter expression in 293 cells was high after transfection of the open reading frame. The transporter showed asymmetric kinetic properties in comparison of the influx and efflux of model substrates, thyroxine (T4), triiodothyronine (T3), and estradiol-glucuronide (E2G). Thyroxine and T3 inhibited the influx of E2G, but E2G did not inhibit thyroxine influx, and T3 only weakly inhibited the influx of T4. Extracellular E2G stimulated the transefflux of intracellular T4. Blood-brain barrier-specific anion transporter type 1 is a novel organic anion transporter that is a sodium-independent exchanger that may participate in the active efflux of iodothyronines and steroid conjugates at the BBB.

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Section: Discussionsupporting

confidence: 61%

Blood—Brain Barrier Genomics and Cloning of a Novel Organic Anion Transporter

Cai¹,

Li²,

Boado³

et al. 2007

J Cereb Blood Flow Metab

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“…Cultured cells were Journal of Cell Science 115 (23) All have biotinylated U at the 3′ end. The sequences for rat zipcode, A2RE and AURE are from Schedlich et al, 1997and Munro et al, 1999, and Hamilton et al, 1999and Tsukamoto et al, 1996 incubated in 0.1% Triton X-100 for 10 minutes, 0.2% fish skin gelatin for 15 minutes, primary antibody for 30 minutes and secondary antibody for 30 minutes. Tissue sections and cultured cells were mounted in Vectashield containing 4′-6-diamidino-2-phenylindole (DAPI, Molecular Probes Inc, Portland, OR).…”

Section: Fluorescence Immunostainingmentioning

confidence: 99%

RNA trafficking and stabilization elements associate with multiple brain proteins

Snee

Kidd

Munro³

et al. 2002

Journal of Cell Science

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Two of the best understood somatic cell mRNA cytoplasmic trafficking elements are those governing localization of β-actin and myelin basic protein mRNAs. These cis-acting elements bind the trans-acting factors fibroblast ZBP-1 and hnRNP A2, respectively. It is not known whether these elements fulfil other roles in mRNA metabolism. To address this question we have used Edman sequencing and western blotting to identify six rat brain proteins that bind the β-actin element (zipcode). All are known RNA-binding proteins and differ from ZBP-1. Comparison with proteins that bind the hnRNP A2 and AU-rich response elements, A2RE/A2RE11 and AURE, showed that AURE and zipcode bind a similar set of proteins that does not overlap with those that bind A2RE11. The zipcode-binding protein, KSRP, and hnRNP A2 were selected for further study and were shown by confocal immunofluorescence microscopy to have similar distributions in the central nervous system, but they were found in largely separate locations in cell nuclei. In the cytoplasm of cultured oligodendrocytes they were segregated into separate populations of cytoplasmic granules. We conclude that not only may there be families of trans-acting factors for the same cis-acting element, which are presumably required at different stages of mRNA processing and metabolism, but independent factors may also target different and multiple RNAs in the same cell.

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“…Several other HIF-1a downstreamregulated proteins and their association with cancer is less known, although CA-IX and Glut-1 expression has been reported to correlate with poor response to adjuvant treatments [46][47][48]. Overexpression of CA-IX and Glut-1 has been established in higher-grade brain tumours [41,[49][50][51][52][53][54][55][56][57] and malignant glioma cell lines [56,58], whereas CA-IX expression in malignant gliomas has been shown to predict radiological response and survival of patients treated with bevacizumab and irinotecan [59]. High-grade gliomas were also more likely to be immunohistochemically positive for HIF-1a, VEGF, Glut-1 and CA-IX than are low-grade tumours [41,49].…”

Section: Overview Of Hypoxia and Its Importancementioning

confidence: 99%