Differential expression of CD8α and CD8β associated with MHC-restricted and non-MHC-restricted cytolytic effector cells

Baume, D; Caligiuri, Michael A.; Manley, Thomas; Daley, John; Ritz, Jérôme

doi:10.1016/0008-8749(90)90260-x

Cited by 72 publications

(38 citation statements)

References 34 publications

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“…This finding is somewhat surprising and may help in explaining some experimental and/or clinical observations. The ␣␣ form of CD8 is mostly expressed on NK cells (43,62) and is usually associated with a poor proliferative response to mitogenic stimulation and preferential display of non-MHC-restricted cytotoxic activity (43,63). Indeed, in several repeated attempts we never succeeded in stabilizing in vitro long term CD3 ϩ CD8 ϩ CD5 Ϫ lines or clones; the inability of these cells to undergo substantial in vitro expansion was apparently not due to the production of immunomodulatory cytokines, such as TGF-␤ (data not shown).…”

Section: Discussionmentioning

confidence: 99%

CD8+αβ+ T Cells That Lack Surface CD5 Antigen Expression Are a Major Lymphotactin (XCL1) Source in Peripheral Blood Lymphocytes

Stievano

Tosello

Marcato

et al. 2003

The Journal of Immunology

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To better characterize the cellular source of lymphotactin (XCL1), we compared XCL1 expression in different lymphocyte subsets by real-time PCR. XCL1 was constitutively expressed in both PBMC and CD4+ cells, but its expression was almost 2 log higher in CD8+ cells. In vitro activation was associated with a substantial increase in XCL1 expression in both PBMC and CD8+ cells, but not in CD4+ lymphocytes. The preferential expression of XCL1 in CD8+ cells was confirmed by measuring XCL1 production in culture supernatants, and a good correlation was found between figures obtained by real-time PCR and XCL1 contents. XCL1 expression was mostly confined to a CD3+CD8+ subset not expressing CD5, where XCL1 expression equaled that shown by γδ+ T cells. Compared with the CD5+ counterpart, CD3+CD8+CD5− cells, which did not express CD5 following in vitro activation, showed preferential expression of the αα form of CD8 and a lower expression of molecules associated with a noncommitted/naive phenotype, such as CD62L. CD3+CD8+CD5− cells also expressed higher levels of the XCL1 receptor; in addition, although not differing from CD3+CD8+CD5+ cells in terms of the expression of most α- and β-chemokines, they showed higher expression of CCL3/macrophage inflammatory protein-1α. These data show that TCR αβ-expressing lymphocytes that lack CD5 expression are a major XCL1 source, and that the contribution to its synthesis by different TCR αβ-expressing T cell subsets, namely CD4+ lymphocytes, is negligible. In addition, they point to the CD3+CD8+CD5− population as a particular T cell subset within the CD8+ compartment, whose functional properties deserve further attention.

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Section: Discussionmentioning

confidence: 99%

CD8+αβ+ T Cells That Lack Surface CD5 Antigen Expression Are a Major Lymphotactin (XCL1) Source in Peripheral Blood Lymphocytes

Stievano

Tosello

Marcato

et al. 2003

The Journal of Immunology

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“…Besides this, NK-cell activation also results in either down or upregulation of different receptors constitutively expressed by resting mature NK-cells. Accordingly, it has been reported that upon in vitro activation, expression of CD2, CD11a/CD18, CD26, CD44, CD54, and CD58 increases (19 -21), whereas the surface levels of CD7 (22), CD11b-CD11c/CD18 and CD16/TCRzeta chain complex decrease (9,10,19,23,24,26), reactivity for other molecules such as CD8 remaining stable (25). Activation-related changes on the expression of some other molecules still remain controversial, probably depending on the type and intensity of the stimuli.…”

Section: Discussionmentioning

confidence: 99%

The “ex Vivo” Patterns of CD2/CD7, CD57/CD11c, CD38/CD11b, CD45RA/CD45RO, and CD11a/HLA-DR Expression Identify Acute/Early and Chronic/Late NK-Cell Activation States

Lima

Almeida

Teixeira

et al. 2002

Blood Cells, Molecules, and Diseases

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ABSTRACT:To define a dynamic sequence of phenotypic changes related to early and late phases of NK-cell activation, we have analyzed by four-color flow cytometry the immunophenotype of normal blood NK-cells from 12 healthy individuals and compared it with those from 15 patients with acute viral infections and 15 patients with either chronic infections or tumors. Although a great interindividual variability was found, nonstimulated CD56 ϩ NK-cells, present in normal blood samples, usually were CD2 Ϫ/ϩlo , CD7 ϩhi , HLA-DR Ϫ , CD11b ϩ , CD38 ϩ , CD11a ϩhi , CD45RA ϩhi , and CD45RO Ϫ , the expression of CD11c and CD57 being heterogeneous and variable. Recently activated NK-cells, herein corresponding to NK-cells from patients with acute viral infections, displayed a pattern of expression of CD2/CD7 similar to that referred to above, but they typically showed higher levels of CD11a, CD38, and HLA-DR, as well as downregulation of CD11b and CD45RA, accompanied in some cases by coexpression of CD45RO; in addition, these NK-cells were CD11c ϩ and CD57 Ϫ/ϩlo . Late-activated NK-cells, represented by NK-cells present in patients with chronic infections and tumors, converted into a CD2 ϩhi /CD7 Ϫ/ϩlo immunophenotype and expressed heterogeneously low levels of CD38 and CD11b; moreover, they were CD57 ϩ and CD11c Ϫ/ϩ . At this stage, most NK-cells had already reverted into their original CD45RA ϩ /CD45RO Ϫ /HLA-DR Ϫ phenotype. In summary, we show that the patterns of expression of CD2/CD7, CD57/CD11c, CD38/CD11b, CD45RA/CD45RO, and CD11a/HLA-DR may help us to define the immunophenotypic profiles associated with early and late NK-cell activation phases in "in vivo" models. © 2002 Elsevier Science (USA)

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“…9 In contrast to CD8 + T cells, where CD8 is composed of both α and β chains, CD8 expressed on the surface of NK cells is composed of predominantly CD8-α chains. 14 …”

Section: Characterization Of Nk Cellsmentioning

confidence: 99%

Interactions Between Natural Killer Cells, Cortisol and Prolactin in Malaria During Pregnancy

Mavoungou¹

2006

Clinical Medicine & Research

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Natural killer cells derived from pluripotent hematopoietic stem cells are important cells of the immune system that have two main functions: a cytolytic activity and a cytokine-producing capacity. These functions are tightly regulated by numerous activating and inhibitory receptors, including newly discovered receptors that selectively trigger the cytolytic activity in a major histocompatibility complex independent manner. Based on their defining function of spontaneous cytotoxicity without prior immunization, natural killer (NK) cells have been thought to play a critical role in immune surveillance and cancer therapy. New insights into NK cell biology have suggested their major roles in the control of infections, particularly in Plasmodium falciparum infection and in fetal implantation. P. falciparum is the main protozoan parasite responsible for malaria causing 200-300 million clinical cases and killing over 3 million people each year. This review provides an update on NK cell function, ontogeny and biology in order to better understand the role of NK cells in pregnancy in regions where malaria is endemic. Understanding mechanisms of NK cell functions may lead to novel therapeutic strategies for the treatment of human disease, in general, and particularly in the fight against malaria.

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Differential expression of CD8α and CD8β associated with MHC-restricted and non-MHC-restricted cytolytic effector cells

Cited by 72 publications

References 34 publications

CD8+αβ+ T Cells That Lack Surface CD5 Antigen Expression Are a Major Lymphotactin (XCL1) Source in Peripheral Blood Lymphocytes

CD8+αβ+ T Cells That Lack Surface CD5 Antigen Expression Are a Major Lymphotactin (XCL1) Source in Peripheral Blood Lymphocytes

The “ex Vivo” Patterns of CD2/CD7, CD57/CD11c, CD38/CD11b, CD45RA/CD45RO, and CD11a/HLA-DR Expression Identify Acute/Early and Chronic/Late NK-Cell Activation States

Interactions Between Natural Killer Cells, Cortisol and Prolactin in Malaria During Pregnancy

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