Differential expression of claudin‑4, occludin, SOX2 and proliferating cell nuclear antigen between basaloid squamous cell carcinoma and squamous cell carcinoma

Sato, Fuyuki; Bhawal, Ujjal K.; Tojyo, Itaru; Fujita, Shigeyuki; Murata, Shin‐ichi; Muragaki, Yasuteru

doi:10.3892/mmr.2019.10417

Cited by 7 publications

(15 citation statements)

References 34 publications

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“…Ten 4 μm serial sections were prepared for staining and incubated with primary antibodies for 2 h. Immunohistochemistry was performed using a Discovery Auto-Stainer with automated protocols (Ventana Medical Systems, Inc., Tucson, AZ, USA; Roche, Mannheim, Germany) as previously described [ 37 , 38 ]. The intensities of DEC1, DEC2, SOX2, c-MYC and vimentin were determined by qualitative assessment of three levels: weak (1), moderate (2) and strong (3), as previously described [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

“…The cells were lysed using M-PER protein lysis buffer (Thermo Fisher Scientific, Inc.). Protein determination was performed using the bicinchoninic method [ 38 ], and the 40 µg proteins of the total cell lysates were run on SDS-polyacrylamide gels followed by western blotting using standard procedures. A WesternBright Sirius Kit (Advansta, CA, USA) was used for antibody detection, and an AE-9300 Ez capture MG (ATTO, Tokyo, Japan) was used for image data capture.…”

Section: Methodsmentioning

confidence: 99%

“…We prepared three independent RNA samples from HeLa and SiHa cells. The total RNA was isolated and first-strand cDNA was synthesized as previously described [ 38 ]. Real-time PCR was performed using SYBR Green Master Mix (Bio-Rad Laboratories, Inc., Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

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Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis

et al. 2020

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Basic helix-loop-helix (BHLH) transcription factors differentiated embryonic chondrocyte gene 1 (DEC1) and gene 2 (DEC2) regulate circadian rhythms, apoptosis, epithelial mesenchymal transition (EMT), invasions and metastases in various kinds of cancer. The stem cell markers SOX2 and c-MYC are involved in the regulation of apoptosis and poor prognosis. In cervical cancer, however, their roles are not well elucidated yet. To determine the function of these genes in human cervical cancer, we examined the expression of DEC1, DEC2, SOX2 and c-MYC in human cervical cancer tissues. In immunohistochemistry, they were strongly expressed in cancer cells compared with in non-cancerous cells. Notably, the strong rate of DEC1 and SOX2 expressions were over 80% among 20 cases. We further examined the roles of DEC1 and DEC2 in apoptosis. Human cervical cancer HeLa and SiHa cells were treated with cisplatin—HeLa cells were sensitive to apoptosis, but SiHa cells were resistant. DEC1 expression decreased in the cisplatin-treated HeLa cells, but had little effect on SiHa cells. Combination treatment of DEC1 overexpression and cisplatin inhibited apoptosis and affected SOX2 and c-MYC expressions in HeLa cells. Meanwhile, DEC2 overexpression had little effect on apoptosis and on SOX2 and c-MYC expressions. We conclude that DEC1 has anti-apoptotic effects and regulates SOX2 and c-MYC expressions on apoptosis.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis

et al. 2020

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“…The PD-L1, P53 and CK17 expression OSCC tissues were evaluated from serial deparaffinized sections. The OSCC biopsy specimens had been fixed with formalin from 24 to 48 h at room temperature and treated with routine processing as in a previous study [12, 13]. Four micrometer-thick sections of paraffin-embedded tissues were mounted on precoated slides and air-dried overnight at 58°C.…”

Section: Methodsmentioning

confidence: 99%

“…Four micrometer-thick sections of paraffin-embedded tissues were mounted on precoated slides and air-dried overnight at 58°C. The serial sections were prepared for staining and were incubated with primary antibodies for 12 h. Immunohistochemistry (IHC) was performed using a Discovery Auto-Stainer with automated protocols (Ventana Medical Systems, Inc.; Roche) as previously described [13, 14].…”

Section: Methodsmentioning

confidence: 99%

PD-L1 expression correlated with p53 expression in oral squamous cell carcinoma

Tojyo

Shintani

Nakanishi

et al. 2019

Maxillofac Plast Reconstr Surg

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Background: Programmed cell death ligand 1 (PD-L1) is an immune checkpoint molecule that attenuates the immune response. PD-L1 contributes to failed antitumor immunity; thereby, blockade of PD-L1 with monoclonal antibody enhances the immune response. Recently, it was reported that PD-L1 was regulated by protein 53 (p53). Besides, cytokeratin 17 (CK17) is thought to be a diagnostic marker of oral squamous cell carcinoma (OSCC). Our aim was to evaluate the correlation between the immunohistochemical expression of PD-L1, p53 and CK17 with clinicopathological characteristics and disease-specific survival in patients with OSCC. Methods: A total of 48 patients with OSCC were included in this study. Immunohistochemical staining was performed to evaluate the correlation among the expressions of PD-L1, p53 and CK17, and furthermore the correlation among various clinicopathological factors, PD-L1, p53 and CK17. Results: The positive rate of p53, CK17, PD-L1 (tumor cells) and PD-L1 (tumor-infiltrating lymphocytes) was 63.2%, 91.7%, 48.9% and 57.1%. A statistically significant correlation between p53 expression and T stage and TNM stage (p = 0.049, p = 0.03, respectively) was observed. Also, a statistically significant correlation between p53 and PD-L1 (TCs) expression (p = 0.0009) was observed. Five-year disease-specific survival rate was not significantly correlated with gender, TNM stage, p53 expression, PD-L1 expression and CK17 expression. Conclusion: The expression of p53 and PD-L1 shows significantly positive correlation in oral squamous cell carcinoma in tumor cells. Also, a significant correlation between p53 expression and T stage and TNM stage was observed. No other significant correlation between PD-L1 staining or CK17 and clinical or pathologic characteristics was identified.

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The relationship of immunohistochemical SOX-2 staining with histopathological diagnosis in patients with abnormal colposcopic findings

Atıgan¹,

Kılıç

Karakaya

et al. 2023

Histochem Cell Biol

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Differential expression of claudin‑4, occludin, SOX2 and proliferating cell nuclear antigen between basaloid squamous cell carcinoma and squamous cell carcinoma

Cited by 7 publications

References 34 publications

Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis

Potential Role of DEC1 in Cervical Cancer Cells Involving Overexpression and Apoptosis

PD-L1 expression correlated with p53 expression in oral squamous cell carcinoma

The relationship of immunohistochemical SOX-2 staining with histopathological diagnosis in patients with abnormal colposcopic findings

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