2014
DOI: 10.1002/term.1949
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Differential expression of GAP-43 and neurofilament during peripheral nerve regeneration through bio-artificial conduits

Abstract: Nerve conduits are promising alternatives for repairing nerve gaps; they provide a close microenvironment that supports nerve regeneration. In this sense, histological analysis of axonal growth is a determinant to achieve successful nerve regeneration. To evaluate this process, the most-used immunohistochemical markers are neurofilament (NF), β-III tubulin and, infrequently, GAP-43. However, GAP-43 expression in long-term nerve regeneration models is still poorly understood. In this study we analysed GAP-43 ex… Show more

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Cited by 97 publications
(96 citation statements)
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“…Quantification of the histochemical signal as determined by percentage of area occupied by positively stained structures and intensity of the signal was performed in each sample for each histochemical method using the ImageJ software (National Institutes of Health, USA) as previously described (Carriel et al 2013, 2017). Briefly, intensity was determined by randomly selecting ten points of each image using the multi-point tool of the software, and the average intensity was calculated by subtracting the white signal to the value calculated by the program.…”
Section: Methodsmentioning
confidence: 99%
“…Quantification of the histochemical signal as determined by percentage of area occupied by positively stained structures and intensity of the signal was performed in each sample for each histochemical method using the ImageJ software (National Institutes of Health, USA) as previously described (Carriel et al 2013, 2017). Briefly, intensity was determined by randomly selecting ten points of each image using the multi-point tool of the software, and the average intensity was calculated by subtracting the white signal to the value calculated by the program.…”
Section: Methodsmentioning
confidence: 99%
“…Therefore, those results indicate that short‐term OGD treatment facilitates the dendritic morphogenesis. The expression level of growth‐associated protein‐43 (GAP‐43), a marker for neuronal growth (Carriel et al, 2014), was analyzed using western blot in different OGD treatment groups. Higher‐than‐control GAP‐43 level was observed in 2 h and 4 h OGD treatment, whereas GAP‐43 expression showed no significant difference in 6 h and 8 h OGD groups (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…One of the possible limitations of the present study is the quantification method used to determine the amount of ECM constituents of the different samples. Although this is a simple straightforward method that has been extensively used by our research group, 4 , direct quantification of the stained areas could preferentially reflect the mean signal intensity of positively stained areas as suggested by Gonzalez and cols, thus failing to accurately report ECM components preservation after treatment in the whole tissue. Therefore, it could be used as an indicator of the retained portion of a specific ECM component after preservation.…”
Section: Discussionmentioning
confidence: 99%
“…For the ECM analysis, three tissue sections were obtained from each sample. Three microphotographs were taken from each sample using a Nikon Eclipse 90i microscope (Nikon corp., Tokyo, Japan), and quantification of the staining signal of each ECM component was performed by using the ImageJ software (National Institutes of Health, USA) as previously published . Briefly, 15 measurements were obtained from the nine images taken of each sample type and the positive signal was automatically quantified by the software.…”
Section: Methodsmentioning
confidence: 99%