Differential expression of genes in greenbug (<i>Schizaphis graminum</i> Rondani) treated by imidacloprid and RNA interference

Ma, Kangsheng; Liu, Jun-Jie; Lu, Lu; Chen, Xiling; Zhang, Shouping; Gao, Xiwu

doi:10.1002/ps.5293

Cited by 24 publications

(17 citation statements)

References 40 publications

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“…Wheat ( Triticum aestivum L.) is an important food crop grown worldwide and used as a staple food in many countries 1 . The wheat aphid ( Schizaphis graminum Rondani) (Hemiptera: Aphididae) is a destructive insect pest of many cereal crops and causes severe damage by phloem sap ingestion and virus transmission 2–5 . However, efforts to control this pest by spraying crops with high doses of multiple pesticides pose serious risks to the environment and have resulted in pesticide resistance 6–8 .…”

Section: Introductionmentioning

confidence: 99%

Seed priming with calcium chloride enhances wheat resistance against wheat aphid Schizaphis graminumRondani

Wang

Song

et al. 2021

Pest Management Science

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BACKGROUND Calcium is an essential macronutrient for plant growth. Although it has been shown that exogenous Ca application can increase plant resistance to abiotic stress, little is known about its potential to enhance plant tolerance to biotic stress. Here, we investigated whether pretreatment of wheat (Triticum aestivum L.) seeds with calcium chloride (CaCl2) improves plant resistance against wheat aphid (Schizaphis graminum Rondani). The developmental time, population size, feeding behavior of aphids on plants grown from CaCl2‐ and water‐pretreated seeds, and plant defense responses to aphid attack were investigated. RESULTS Seed pretreatment with CaCl2 extended aphid development time and reduced aphid population size and feeding efficiency. In addition, the pretreatment significantly increased the concentration of Ca2+ in wheat leaves, and upregulated expression levels of TaCaM genes and callose synthase genes (TaGSL2, TaGSL8, TaGSL10, TaGSL12, TaGSL19, TaGSL22 and TaGSL23). Callose concentration in the leaves of plants grown from CaCl2‐pretreated seeds increased significantly upon aphid attack. Further, callose deposition was observed mainly in the phloem. CONCLUSION These results suggest that seed pretreatment with CaCl2 primes the plant response against wheat aphid attack, leading to modulation of callose deposition in the phloem in response to aphid attack. © 2021 Society of Chemical Industry.

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Section: Introductionmentioning

confidence: 99%

Seed priming with calcium chloride enhances wheat resistance against wheat aphid Schizaphis graminumRondani

Wang

Song

et al. 2021

Pest Management Science

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“…At last, PCR products were purified (AMPure XP system) and library products were then ready for sequencing analysis via Illumina HiseqTM 4000 (Beijing Novogene, Beijing, China) using paired‐end technology in a single run (Zhang et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…Then PCR was performed with Phusion High-Fidelity DNA polymerase, Universal PCR primers and Index (X) Primer. At last, PCR products were purified (AMPure XP system) and library products were then ready for sequencing analysis via Illumina HiseqTM 4000 (Beijing Novogene, Beijing, China) using paired-end technology in a single run (Zhang et al 2019).…”

Section: Insect Culture and Collectionmentioning

confidence: 99%

De novo assembly of the transcriptome for Greenbug (Schizaphis graminum Rondani) and analysis on insecticide resistance‐related genes

Liu

et al. 2019

Entomological Research

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The greenbug, Schizaphis graminum Rondani is a major pest species of wheat crops. In this study, a transcriptome sequencing, and the expression of the 12 genes related to insecticide resistance were conducted in S. graminum. The sequencing and subsequent bioinformatics analysis outputed 46,593 unigenes, among which 28,289 unigenes were annotated to corresponding functions by blasting with high homologous genes in database, giving annotation rate of 60.72%. To gain insight into the mechanism of insecticide resistance, the expression of the 12 genes related to insecticide resistance for S. graminum was investigated. The expression level of aminopeptidase N (AN), cytochrome P450 (CYP), acetylcholinesterase 1 (AC), catalase (CAT), cytochrome c oxidas (CCC), GABA receptor (GABA), glutathione S‐transferase (GST) were highest in the apterous nymphs among different developmental stages; The expression level of AN, CBL, CYP, CA, SD, and GST were relatively more abundant in the abdomen compared to head and throax. The results could give out the key information about the relationship between the expression of these genes in different developmental stages, tissues, treatments and metabolism of insecticides. These genes that were co‐up‐regulated significantly in third instar nymphs of S. graminum induced by imidacloprid, were consistent with their putative involvement in insecticide resistance. This provides most comprehensive transcriptome data for S. graminum to further study and managenment.

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“…Total RNA, first strand cDNA synthesized, SYBR Green Master Mix (miScript SYBR Green PCR Kit, Qiagen) and qPCR procedure determined was described as previously. 33 Relative expression quantification was calculated by the 2 −∆∆Ct method. 34 U6 snRNA was used to normalize expression quantiy of miRNAs as an endogenous control.…”

Section: Quantitative Real-time Pcr (Qpcr) Validationmentioning

confidence: 99%

Differentially expressed microRNAs under imidacloprid exposure and identification in Sitobion avenae

Chen

Liu

et al. 2019

Preprint

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Backgroud: MicroRNAs (miRNAs), which are as short single-stranded non-coding RNAs, coudl regulate the expression of target genes, especially regulation or metabolism of endogenous or xenobiotic compounds. Results: The de novo assembly of the transcriptomes was obtained through Illumina short-read sequencing technology in Sitobion avenae. 57 miRNAs, of which 36 were known and 21 were novel were identified. Quantitative expression levels of miRNA showed that the expression of 5 miRNAs were significant up-regulation, and the expression of 11 miRNAs were significant down-regulation in the nymph of S. avenae treated by imidacloprid in comparison with the control, respectively. The candidate transcript target genes in S. avenae that could be regulated by these miRNAs were also carried out. The functions of the miRNAs, which could potentially regulate the target genes participated in the metabolism, regulatory or detoxification of S. avenae were clarified based on Gene Ontology and KEGG pathway. The effects of the miRNAs identified api-miR-1000, api-miR-316, and api-miR-iab-4 on susceptibility of S. avenae to imidacloprid was determined. The abundance of api-miR-1000, api-miR-316, and api-miR-iab-4 modulated by the addition of their own inhibitors to the artificial diet could change significantly the susceptibility of S. avenae to imidacloprid, which further proved that the regulatory affect of these miRNAs on regulation or metabolism of insecticides. Conclusion: It suggested that differentially expressed microRNAs under the stress of imidacloprid could play critical regulatory role in the resistance of S. avenae to imidacloprid.

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Differential expression of genes in greenbug (Schizaphis graminum Rondani) treated by imidacloprid and RNA interference

Cited by 24 publications

References 40 publications

Seed priming with calcium chloride enhances wheat resistance against wheat aphid Schizaphis graminumRondani

Seed priming with calcium chloride enhances wheat resistance against wheat aphid Schizaphis graminumRondani

De novo assembly of the transcriptome for Greenbug (Schizaphis graminum Rondani) and analysis on insecticide resistance‐related genes

Differentially expressed microRNAs under imidacloprid exposure and identification in Sitobion avenae

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