Differential Expression of Histone H3.3 Genes and Their Role in Modulating Temperature Stress Response inCaenorhabditis elegans

Abstract

Histone H3.3, a major variant of canonical histone H3, is highly conserved and essential for viability or fertility in most lineages. In Caenorhabditis elegans, H3.3 is expressed from five genes. Here, Delaney...

Local inhibition of PRC2 activity by H3.3K27M drives DNA replication defects through misregulation of the JNK pathway

“…on the chromatin in C. elegans germ cells, and the H3.3-characteristic depletion from the X 159! chromosome is no longer evident 30 . Introducing the K27M mutation into this H3-like histone 160!…”

“…We introduced the K27M mutation into the H3.3 gene his-72, one of five C. elegans H3.3 genes, 98! which is ubiquitously expressed and non-essential 30 . His-72 transcript levels are 50 times lower 99!…”

“…We found that introduction of K27M into the 413! germline-specific H3.3 gene his-74, which is expressed at lower levels compared to his-72 30 , did 414! not result in the replicative phenotype (data not shown), suggesting that in this case chromatin 415!…”

“…cells at all developmental stages both in the soma and in the germline, throughout the 477! manuscript 30,34 . All genome edits were generated at the endogenous loci of his-72 and kgb-1 478!…”

Histone variant H3.3 residue S31 is essential for Xenopus gastrulation regardless of the deposition pathway

“…As sterility could simply reflect a shortage of maternal H3.3 to replace protamine from sperm chromatin after fertilization, the most parsimonious hypothesis suggests that the nature of the variant itself might not be critical. Similarly, H3.3 is not essential in Caenorhabditis elegans, where its removal is not lethal but reduces fertility and viability in response to stress 37 . However, in Arabidopsis thaliana, replicative and non-replicative H3 variants are essential.…”