Differential expression of insecticide resistance genes in Anopheles gambiae from Northern Nigeria

Abdu, Habibu U.; Hapca, Simona M.; Dauda, M; Deeni, Yusuf Y.

doi:10.1016/j.jbiotec.2018.06.067

Cited by 2 publications

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“…in the lowland forest vegetation. This agrees with the findings in Ibadan, northern Nigeria, Kilokope in Togo, Mali, Cote d'Ivoire, southeast Benin Republic, Burkina Faso, southwest and northern Cameroon (Awolola, 2007;Namountougou et al, 2012;Cisse et al, 2015;Etang et al, 2016;Yahouedo et al, 2016;Abdu et al, 2017;Camara et al, 2018;Ketoh et al, 2018). The KDR gene band on sample 2 was very faint and on sample 4, it was fainter.…”

Section: Discussionsupporting

confidence: 91%

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2023

IJENR

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Comprehending the genetic arrangement of resistant mosquito populations is essential for addressing biological and public health concerns as, spread of insecticide resistant alleles. Anopheles gambiae sensu lato, which spread malaria parasites comprises genetically different but morphologically indistinguishable species. This study: determine presence of resistant genes, in the lowland forest, mangrove and fresh water swamp in Rivers state, Niger delta. Sample collection was done from May to December. Laboratoryreared larvae collected from their breeding sites, were used for the molecular bioassay. Resistant genes were determined from DNAs extracted from the tissues of the samples and subjected to polymerase chain reaction (PCR), to identify knockdown and acetylcholinesterase resistance mechanisms. Acetylcholinesterase resistance mutation with accession number OQ802845 was found in all the three vegetations while knockdown resistance mutation was scantily found in the lowland forest vegetation only. The study makes available reference base-line data of various insecticide resistant genes in the Niger delta.

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Section: Discussionsupporting

confidence: 91%

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2023

IJENR

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“…However, yeast and bacteria have only a single Gcl gene. − In insects, studies in Drosophila melanogaster revealed that DmGcl is also composed of a catalytic subunit, DmGclc, and a modifier subunit, DmGclm. , Recently, genes encoding Gclc and Gclm have been identified in Anopheles gambiae and S. litura . , However, only one Gcl gene was found in the silkworm genome . In this study, genes encoding Gclc and Gclm in FAW were cloned by RT-PCR.…”

Section: Discussionmentioning

confidence: 95%

“…39,40 Recently, genes encoding Gclc and Gclm have been identified in Anopheles gambiae and S. litura. 23,41 However, only one Gcl gene was found in the silkworm genome. 42 In this study, genes encoding Gclc and Gclm in FAW were cloned by RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Glutamate Cysteine Ligase Genes in Tolerance to Emamectin Benzoate in Spodoptera frugiperda and Their Putative Regulatory Mechanisms

Cao,

Wei,

et al. 2023

J. Agric. Food Chem.

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As the rate-limiting enzyme in de novo Glutathione (GSH) biosynthesis, the mammalian glutamate cysteine ligase (Gcl) catalytic (Gclc) and modifier (Gclm) subunits are regulated at multiple levels, whereas the function and regulatory mechanism of insect Gcl remain to be explored. In this study, we identified and characterized SfGclc and SfGclm in Spodoptera frugiperda . SfGclc and SfGclm were highly expressed in the hindgut and relatively less expressed in other tissues. The exposure of the third instar larvae to LC30 of emamectin benzoate (EMB) significantly reduced the GSH content with a concomitant upregulation of SfGclc and SfGclm. Further in vivo pretreatment with L-BSO, the Gcl inhibitor, increased the susceptibility of S. frugiperda to EMB. Consistently, overexpression of SfGclc and SfGclm increased the Sf9 cell viability under EMB treatment. Finally, both RNAi and the dual-luciferase reporter assay in Sf9 cells revealed that SfGclc is regulated by transcription factor CncC. These data provide insights into the function and regulatory mechanism of insect Gcl, and they imply that disruption of the redox homeostasis might be a practical strategy to enhance the insecticidal activity of EMB and other insecticides.

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Differential expression of insecticide resistance genes in Anopheles gambiae from Northern Nigeria

Cited by 2 publications

References 0 publications

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Involvement of Glutamate Cysteine Ligase Genes in Tolerance to Emamectin Benzoate in Spodoptera frugiperda and Their Putative Regulatory Mechanisms

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