Differential expression of interferon inducible protein: Guanylate binding protein (GBP1 &amp; GBP2) in severe dengue

Mariappan, Vignesh; Adikari, Shalinda; Lokesh, S.; Easow, Joshy M; Pillai, Agieshkumar Balakrishna

doi:10.1016/j.freeradbiomed.2022.11.037

Cited by 7 publications

(3 citation statements)

References 50 publications

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“…It had been demonstrated that GBP1 inhibited hepatitis C virus replication by hydrolyzing GTPase in knockout and overexpression cell models (Itsui et al 2009). This antiviral mechanism of GBP1 was also confirmed in subsequent studies on dengue virus (Mariappan et al 2023), influenza virus (Nordmann et al 2012), hepatitis E virus (Glitscher et al 2021, and swine fever virus (Li et al 2016). Furthermore, researchers have observed that GBP1 can inhibit the spread of pathogenic microbial infections by activating inflammasomes, inducing pyroptosis, or participating in autophagy (Fisch et al 2019).…”

Section: Introductionmentioning

confidence: 63%

Guanylate-Binding Protein 1 (GBP1) Enhances IFN-α Mediated Antiviral Activity against Hepatitis B Virus Infection

Li,

Luo,

et al. 2024

Polish Journal of Microbiology

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Interferon-alpha (IFN-α) is a first-line drug for treating chronic hepatitis B (CHB). Guanylate-binding protein 1 (GBP1) is one of the interferon-stimulating factors, which participates in the innate immunity of the host and plays an antiviral and antibacterial role. In this study, we explored how GBP1 is involved in IFN-α antiviral activity against HBV. Before being gathered, HepG2-NTCP and HepG2 2.15 cells were transfected with the wild-type hGBP1 plasmid or si-GBP1, respectively, and followed by stimulation with Peg-IFNα-2b. We systematically explored the role of GBP1 in regulating HBV infection in cell models. Additionally, we also examined GBP1 levels in CHB patients. GBP1 activity increased, and its half-life was prolonged after HBV infection. Overexpression of GBP1 inhibited the production of HBsAg and HBeAg, as well as HBs protein and HBV total RNA levels, whereas silencing of GBP1 inhibited its ability to block viral infections. Interestingly, overexpressing GBP1 co-treatment with Peg-IFNα-2b further increased the antiviral effect of IFN-α, while GBP1 silencing co-treatment with Peg-IFNα-2b partly restored its inhibitory effect on HBV. Mechanistically, GBP1 mediates the anti-HBV response of Peg-IFNα-2b by targeting HBs. Analysis of clinical samples revealed that GBP1 was elevated in CHB patients and increased with Peg-IFNα-2b treatment, while GBP1 showed good stability in the interferon response group. Our study demonstrates that GBP1 inhibits HBV replication and promotes HBsAg clearance. It is possible to achieve antiviral effects through the regulation of IFN-α induced immune responses in response to HBV.

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Section: Introductionmentioning

confidence: 63%

Guanylate-Binding Protein 1 (GBP1) Enhances IFN-α Mediated Antiviral Activity against Hepatitis B Virus Infection

Li,

Luo,

et al. 2024

Polish Journal of Microbiology

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“…SAMHD1 is a host-limiting immune factor that inhibits viral infection and replication by hydrolyzing intracellular dNTPs. Previous studies have shown that SAMHD1 is not only involved in autoimmune regulation, but also TNF-α-induced inflammatory responses [30]. Its activity is negatively regulated by phosphorylation, and when SAMHD1 is phosphorylated, its antiviral ability is weakened [31].…”

Section: Ssp1 Alleviates Pcv2-induced Inflammation In Splenic Lymphoc...mentioning

confidence: 99%

“…Stimulation with HIV-1-induced cytokines increased the high expression of the antiviral factors GBP2 and GBP5 in human cells [32]. GBP2 can directly kill intracellular pathogens and activate downstream inflammasomes after being activated by interferon (IFN), which is an important component of host defense against intracellular pathogens [30]. STAT1 is an important mediator in mediating the IFN response and plays a key role in the immune response.…”

Section: Ssp1 Alleviates Pcv2-induced Inflammation In Splenic Lymphoc...mentioning

confidence: 99%

Whole Transcriptome Analysis of Intervention Effect of Sophora subprostrate Polysaccharide on Inflammation in PCV2 Infected Murine Splenic Lymphocytes

Zhao

Jia

Xie

et al. 2023

CIMB

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(1) Background: Sophora subprostrate, is the dried root and rhizome of Sophora tonkinensis Gagnep. Sophora subprostrate polysaccharide (SSP1) was extracted from Sophora subprostrate, which has shown good anti-inflammatory and antioxidant effects. Previous studies showed SSP1 could modulate inflammatory damage induced by porcine circovirus type 2 (PCV2) in murine splenic lymphocytes, but the specific regulatory mechanism is unclear. (2) Methods: Whole transcriptome analysis was used to characterize the differentially expressed mRNA, lncRNA, and miRNA in PCV2-infected cells and SSP1-treated infected cells. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and other analyses were used to screen for key inflammation-related differentially expressed genes. The sequencing results were verified by RT-qPCR, and western blot was used to verify the key protein in main enriched signal pathways. (3) Results: SSP1 can regulate inflammation-related gene changes induced by PCV2, and its interventional mechanism is mainly involved in the key differential miRNA including miR-7032-y, miR-328-y, and miR-484-z. These inflammation-related genes were mainly enriched in the TNF signal pathway and NF-κB signal pathway, and SSP1 could significantly inhibit the protein expression levels of p-IκB, p-p65, TNF-α, IRF1, GBP2 and p-SAMHD1 to alleviate inflammatory damage. (4) Conclusions: The mechanism of SSP1 regulating PCV2-induced murine splenic lymphocyte inflammation was explored from a whole transcriptome perspective, which provides a theoretical basis for the practical application of SSP1.

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Clinical Relevance of Oxidative Stress Biomarkers in Human Flavivirus Infections as Predictors of Disease Progression and Severity

Van Herreweghe,

De Bruyne,

Hermans

et al. 2024

Reviews in Medical Virology

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Several Flaviviridae constitute an emerging threat to global health because of their continuing spread and the expansion of vector habitats, largely driven by climate change and intensified global travel. Infections can result in severe neurological or visceral pathologies. The relationship between oxidative stress (OS), an imbalance between generated reactive oxygen species and the antioxidant defences of the host, and flavivirus infection has been repeatedly demonstrated in in vitro and animal studies, but measuring biomarkers of oxidative stress in vivo could prove useful in clinical patient management. We summarise the knowledge and prospects of measuring peripheral OS biomarker levels for clinical case management and correlation with disease severity in six important human flavivirus infections (dengue virus (DENV), Japanese encephalitis virus, West Nile virus (WNV), tick‐borne encephalitis virus (TBEV), yellow fever virus and zika virus). We searched the Medline and Web of Science databases for ‘Oxidative Stress’ AND ‘Biomarkers’ AND ‘Flavivirus’, combined with ‘clinical’, ‘in vivo/in vivo’, ‘patient’ and/or ‘disease’ and included 43 peer‐reviewed publications. Correlation between OS and infection has been studied in all six Flaviviridae, but most clinically relevant data are available for DENV, TBEV and WNV. Plasma protein carbonyls, glutathione peroxidase activity and nitrogen monoxide are promising prognostic markers, but their measurement would benefit from methodological harmonisation. Future studies should investigate a broad range of OS biomarkers as predictors of clinically relevant outcomes. We advocate the validation and use of universal or disease‐specific oxidative stress indexes that incorporate the most significant outcomes into one, easy‐to‐use clinical determinant.

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Differential expression of interferon inducible protein: Guanylate binding protein (GBP1 & GBP2) in severe dengue

Cited by 7 publications

References 50 publications

Guanylate-Binding Protein 1 (GBP1) Enhances IFN-α Mediated Antiviral Activity against Hepatitis B Virus Infection

Guanylate-Binding Protein 1 (GBP1) Enhances IFN-α Mediated Antiviral Activity against Hepatitis B Virus Infection

Whole Transcriptome Analysis of Intervention Effect of Sophora subprostrate Polysaccharide on Inflammation in PCV2 Infected Murine Splenic Lymphocytes

Clinical Relevance of Oxidative Stress Biomarkers in Human Flavivirus Infections as Predictors of Disease Progression and Severity

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