Differential expression of L-selectin ligand in the endometrium during the menstrual cycle

Lai, Tsung Hsuan; Shih, Ie Ming; Vlahos, Nikolaos; Ho, Chung Liang; Wallach, Edward E.; Zhao, Yulian

doi:10.1016/j.fertnstert.2004.11.040

Cited by 57 publications

(54 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Studies show that sLe x is expressed in human endometrium throughout the menstrual cycle with a significant increase from early to mid-secretory phases (Ravn et al 1992, Hey & Aplin 1996. Lai et al (2005) showed that the expression of L-selectin ligands that bind to MECA-79 antibody (an antibody that recognizes a sulfate and carbohydrate epitope on a group of endothelial L-selectin ligands) significantly increases throughout the early and mid-secretory stages of the menstrual cycle. Therefore, it is possible that FUT4 participates in the production of these ligands.…”

Section: Discussionmentioning

confidence: 99%

Expression and regulation of fucosyltransferase 4 in human endometrium

Ponnampalam¹,

Rogers²

2008

Reproduction

View full text Add to dashboard Cite

It has been suggested that selectin ligands expressed by the endometrial epithelium are essential for the initial adhesion of the blastocyst to the luminal epithelium of human endometrium. One of the enzymes responsible for the production of selectin ligands is fucosyltransferase 4 (FUT4), a member of a1,3 fucosyltransferases. The aims of the present study were to characterize FUT4 mRNA and protein in human endometrium during the menstrual cycle and to investigate the hormonal regulation of FUT4 whose mRNA expression was quantified by real-time PCR in fresh endometrial tissue from cycling women and protein expression was analyzed by immunohistochemistry and Western blotting. Hormonal regulation of FUT4 transcription was investigated using an endometrial explant system. FUT4 mRNA was significantly upregulated in fresh tissues during early and mid-secretory phases when compared with other phases of the menstrual cycle. FUT4 protein was localized to glandular and luminal epithelium and the expression levels followed the same pattern as for FUT4 mRNA. Our data also show that, in proliferative explants, progesterone significantly increased FUT4 transcription and translation after 24 h in culture. The inductive effect of progesterone on FUT4 transcription was lost after 48 h of treatment. Estrogen did not have any significant effects. These data suggest that the upregulation of selectin ligands in the human endometrium at the time of implantation may be mediated, at least in part, by the regulation of FUT4 expression.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression and regulation of fucosyltransferase 4 in human endometrium

Ponnampalam¹,

Rogers²

2008

Reproduction

View full text Add to dashboard Cite

show abstract

“…Outside this window, the endometrium is resistant to embryo attachment [12]. Lai et al [13,14] described the expression of L-selectin ligand throughout the natural menstrual cycle and controlled ovarian stimulation cycle. Its expression increased from the periovulatory interval to the mid-secretory phase.…”

Section: Discussionmentioning

confidence: 99%

Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity

Foulk

Zdravkovic

Genbačev

et al. 2007

J Assist Reprod Genet

View full text Add to dashboard Cite

Problem Patients with repeated implantation failure (RIF) represent a subgroup of couples who suffer from unexplained infertility. Human blastocysts utilize L-selectin to initiate implantation by binding to endometrial ligands composed of oligosaccharide moieties on the surface glycoproteins. The absence of these ligands could lead to recurrent implantation failure (RIF) in some of these couples. Methods Twenty fertile women and 20 patients with RIF were tested for the presence of the L-selectin ligands by immunohistochemistry. Endometrial biopsies were obtained on the sixth day post ovulation. After fixation, they were dated according to Noyes. Immunolocalization was performed using the MECA-79 antibody which is directed against ligands of L-selectin. Results The fertile group all showed the presence of the L-selectin ligand. Of those with RIF, five were negative for the ligand and never, despite an average of five successive embryo transfers, became pregnant. Fifteen RIF patients were positive for the L-selectin ligand, of whom ten subsequently conceived. As a screening test for RIF patients who lack the ligand, the predictive value was 100% with a sensitivity of 50% and specificity of 100%. The positive predictive value was 100% and negative predictive value is 87%. Conclusions L-selectin and its ligands play a vital role for early human implantation. Screening for the absence of the ligand may help many patients with RIF to avoid undergoing repeated failed treatment cycles.

show abstract

“…It stimulates endothelial cell proliferation, permeability, migration, and assembly into capillary tubes (Okada et al 2014). And the temporal and spatial distribution of VEGF expression in the three components of human endometrium during the menstrual cycle suggests the functional role of angiogenesis in the remodelling process of endometrial tissue (Lai et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Endometrial receptivity has become one of the research hotspots in recent years, and accumulated evidence suggests that hormones (Young 2013), osteopontin (OPN) (Talbi et al 2006), vascular endothelial growth factor (VEGF) (Lai et al 2015), leukaemia-inhibitory factor (LIF) (Miravet-Valenciano et al 2015), prolactin (PRL) (Kautz et al 2015) and other factors participate in the preparation of endometrial receptivity. In addition, intensive research work has been performed to better understand the regulation of the endometrium and its clinical implications to improve implantation in human, and many genes have been reported to influence endometrial development (Strowitzki et al 2006).…”

Section: Doi: 1017221/15/2017-cjasmentioning

confidence: 99%

The study of endometrium at gestational days 5 and 15 in dairy goats (Capra hircus)

Zhang¹,

Liu²,

Ping³

et al. 2017

Czech J. Anim. Sci.

View full text Add to dashboard Cite

Zhang L., Liu X.-R., Han P., Zhou Z.-Q., Cao B.-Y., Song Y.-X. (2017): The study of endometrium at gestational days 5 and 15 in dairy goats (Capra hircus). Czech J. Anim. Sci., 62,[357][358][359][360][361][362][363][364][365][366][367][368] Endometrium receptivity, an absolutely necessary part of successful embryo implantation, and several morphological and biochemical endometrial receptivity biomarkers have already been studied and proposed in some animals. However, to our knowledge, no such a study has as yet been undertaken in dairy goat. In the present study, the serum and uterus of dairy goat were studied at gestational days 5 and 15, the estrogen (E2) and progesterone (P4) concentrations in serum were determined by electrochemiluminescence immunoassay, the surface topography of endometrium was observed by scanning electron microscopy. Furthermore, the estrogen receptors (ER), prolactin (PRL), some marker genes of receptive endometrium, and cell proliferation and apoptosis in the uterus were also detected. Well-formed pinopodes were found on the surface of endometrium at day 15 with higher E2 and P4 concentrations in the serum and higher estrogen receptors ERα and ERβ expression levels and a lower PRL level in the endometrium. Moreover, some expression levels of marker genes of receptive endometrium (OPN, VEGF, LIF, PRLR) were increased at day 15 compared to day 5, but no significant differences were observed in the cell proliferation and apoptosis in the uterus. The results showed that the endometrium reached the receptive state at gestational day 15 in dairy goats. Keywords: endometrial receptivity; pinopodes; gestational day 15 (D15)List of abbreviations: E2 = estrogen, P4 = progesterone, D = gestational day (D0, D1, D5, D15), ER = estrogen receptor, ERα = E2 receptor α, ERβ = E2 receptor β, PR = P4 receptor, PRL = prolactin, PRLR = prolactin receptor, PCNA = proliferating cell nuclear antigen, GHR = growth hormone receptor, IFG1R = insulin-like growth factor 1 receptor, OPN = osteopontin, VEGF = vascular endothelial growth factor, LIF = leukaemiainhibitory factor, RT-qPCR = quantitative real-time polymerase chain reaction, MPA = medroxyprogesterone acetate, FSH = follicle-stimulating hormone, PMSG = pregnant mare's serum gonadotropin, SEM = scanning electron microscopy, PA = paraformaldehyde, PBS = phosphate-buffered saline, ECLI = electrochemiluminescence immunoassay, HE = hematoxylin/eosin, IHC = immunohistochemistry, BSA = bull serum albumin, HRP = horseradish peroxidase, DAB = diaminobenzidine, NC = negative control, TUNEL = terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling, LE = luminal epithelium, GE = glandular epithelium, SC = stromal cell, VE = vascular endothelial cell, IOD = integrated optical density, WOI = window of implantation, EEC = endometrial epithelial cells, ESC = endometrial stromal cells

show abstract

Differential expression of L-selectin ligand in the endometrium during the menstrual cycle

Cited by 57 publications

References 27 publications

Expression and regulation of fucosyltransferase 4 in human endometrium

Expression and regulation of fucosyltransferase 4 in human endometrium

Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity

The study of endometrium at gestational days 5 and 15 in dairy goats (Capra hircus)

Contact Info

Product

Resources

About