Differential expression of microRNA in the serum of patients with polycystic ovary syndrome with insulin resistance

Abstract

Background: Polycystic ovary syndrome (PCOS) is the most common reproductive endocrine disease in women of childbearing age, and insulin resistance is an important etiological mechanism in PCOS. This study revealed the microRNA (miRNA) expression profile of PCOS with insulin resistance and explored the potential biological functions of differentially expressed miRNA.Methods: A total of 76 patients with PCOS and 30 normal healthy women were recruited in the gynecological clinic of the Second Hospital of Tianjin Medical University. We divided the patients with PCOS into a group with insulin resistance (n=46) and a group without insulin resistance (n=30). Peripheral venous serum samples from each group were used for deep sequencing to identify differentially expressed miRNAs. Hierarchical clustering heat maps were used to show differences in miRNA expression. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and target gene network databases were used to explore the potential target genes of differentially expressed miRNAs and to analyze their specific biological functions.Results: A case-control analysis found that the levels of body mass index (BMI), prolactin (PRL), total testosterone (T), fasting blood glucose (FBG), and fasting insulin (INS) in patients with PCOS were higher than those in healthy controls. High BMI, high blood sugar, and hyperinsulinemia were more significant in the PCOS with insulin resistance group than without insulin resistance group. Among the patients with PCOS, miR-122-5p was found to have more significant differences in the PCOS with insulin resistance group. GO and KEGG pathway analysis showed that the identified miRNAs were involved in the regulation of different biological processes, such as signal transduction, negative regulation of GTPase activity, chloride channel complex. The predicted target genes were related to the citrate cycle (TCA cycle) and the biosynthesis of mucin-type O-glycans.Conclusions: Our research demonstrated the use of miRNAs as new biomarkers for the diagnosis, treatment and presented a new strategy to lessen the symptoms of PCOS with insulin resistance.

Non-coding RNA genes modulate PI3K/AKT signaling pathway in polycystic ovary syndrome

“…When compared to the normal control group and those receiving metformin treatment, the PCOS patient group in the current study demonstrated statistically signi cant increases in FBS, HbA1c, fasting insulin, HOMA-IR TC, TG, LDL, and testosterone while there was a signi cant decrease in HDL levels (p-value 0.05). In the same vein, (14) discovered that individuals with PCOS had signi cantly higher levels of (BMI), (T), (FBG), and (INS) of fasting insulin than did healthy controls (p0.05). Like how (15) con rmed our ndings, they discovered that the PCOS group had considerably lower levels of HDL, FSH, and E2 than the controls while signi cantly higher levels of BMI, fasting insulin, HOMA-IR, LDL, TG, TC, testosterone, and LH were present.…”

Exosome Therapy and Photobiomodulation Therapy Regulate mi-RNA 21, 155 Expressions, Nucleus Acetylation and Glutathione in a Polycystic Ovary Oocyte: An In Vitro Study

“…Recent studies have shown that miRNAs play an important role in the development of PCOS, including the regulation of insulin resistance, inflammation, and ovarian function. 13 Among these miRNAs, the miR-17-92 cluster and miR-21 have been found to be dysregulated in PCOS patients. miR-17-92 cluster increases in the granulosa cells of PCOS patients, which may contribute to the development of follicular cysts.…”

“…[10][11][12] Furthermore, miRNA-155 may contribute to the development of PCOS by disrupting normal ovarian function. 13,14 In addition to its role in ovarian function, miRNA-155 has also been implicated in inflammation and insulin resistance, two other key features of PCOS. miRNA-155 can promote inflammation and insulin resistance in various tissues throughout the body.…”