Differential expression of microRNAs miR‐21, miR‐31, miR‐203, miR‐125a‐5p and miR‐125b and proteins PTEN and p63 in verrucous carcinoma of the head and neck

Odar, Katarina; Boštjančič, Emanuela; Gale, Nina; Glavač, Damjan; Zidar, Nina

doi:10.1111/j.1365-2559.2012.04242.x

Cited by 41 publications

(34 citation statements)

References 39 publications

(57 reference statements)

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“…49 Up-regulation of miR-31 was observed in colorectal 50 and head-and-neck tumors. 51 Although the detail mechanism underlying miR-31 dysregulation in tumorigenesis remains to be investigated, studying miR-31 expression in serum could be a potential approach for diagnosis of esophageal SCC. 52 …”

Section: Discussionmentioning

confidence: 99%

Analysis of MicroRNAs in Sputum to Improve Computed Tomography for Lung Cancer Diagnosis

Shen

Liao

Guarnera

et al. 2014

Journal of Thoracic Oncology

115

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Introduction Computed tomography (CT) plays a central role in lung cancer diagnosis. However, CT has relatively low specificity, presenting a challenge in clinical settings. We previously identified 12 microRNAs (miRNAs) whose expressions in tumor tissues were associated with lung cancer. Methods Using quantitative reverse transcriptase polymerase chain reaction, we aimed to identify miRNA biomarkers in sputum that could complement CT for diagnosis of lung cancer. Results In a training set consisting of 66 lung cancer patients and 68 cancer-free smokers, 10 of the 12 miRNAs were differentially expressed between the cases and controls (p ≤ 0.01). From the miRNAs, a logistic regression model was built on the basis of miR-31 and miR-210, both of which had the best prediction for lung cancer, producing an area under receiver operating characteristic curve of 0.83. Combined use of the two miRNAs yielded 65.2% sensitivity and 89.7% specificity, CT had 93.9% sensitivity and 83.8% specificity for lung cancer diagnosis. Notably, combined analysis of the miRNA biomarkers and CT produced a higher specificity than does CT used alone (91.2% versus 83.8%; p < 0.05). The diagnostic performance of the biomarkers was confirmed in a testing set comprising 64 lung cancer patients and 73 cancer-free smokers. Conclusion The sputum miRNA biomarkers might be useful in improving CT for diagnosis of lung cancer, but further independent validation on an external and prospective cohort of patients is required.

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Section: Discussionmentioning

confidence: 99%

Analysis of MicroRNAs in Sputum to Improve Computed Tomography for Lung Cancer Diagnosis

Shen

Liao

Guarnera

et al. 2014

Journal of Thoracic Oncology

115

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“…Concurrent with our examinations, an upregulation of miR-31 in cSCCs was reported (Table 1) (9). Like miR-21, miR-31 is a proto-oncogene (15), but its effect seems to be versatile in several cancers and cancer stages (16)(17)(18)(19)(20). In cSCC, the function of miR-31 has yet to be clarified.…”

Section: Accepted For Publication 14 April 2013mentioning

confidence: 99%

MicroRNA expression differs in cutaneous squamous cell carcinomas and healthy skin of immunocompetent individuals

Bruegger

Kempf²,

Spoerri

et al. 2013

Experimental Dermatology

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Cutaneous squamous cell carcinoma (cSCC) is one of the most common skin cancers, but the influence of microRNA (miRNA) expression has only been sporadically analysed. We hypothesized that miRNAs are differentially expressed in cSCC and hence influence its development. We therefore isolated total miRNA from well-differentiated cSCCs and from controls without SCC. Expression analyses of 12 miRNAs showed three significantly differentially expressed miRNAs. We identified a significant upregulation of the miR-21 and the miR-31, a proto-oncogene like miR-21. While the upregulated expression of miR-21 has been known for some time, the increased expression of miR-31 was never shown so clearly. Furthermore, we showed the upregulation of miRNA-205, which has never been described before. The miR-205 induces specific keratinocyte migration and could be a characteristic marker for cSCC. It has to be determined in following studies whether these upregulated expressions are specific for cSCC and if so, for which cSCC stages.

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“…miR-125a-5p has been shown to be overexpressed in many cancers [12][13][14]. In addition, miR-125-5p is also reported to trigger proliferation, invasion, and migration in tumor cells [15][16][17].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-125a-5p Contributes to Hepatic Stellate Cell Activation through Targeting FIH1

et al. 2016

Cell Physiol Biochem

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Key Words MicroRNA • MiR-125a-5p • Hepatic stellate cells • Liver fibrosis • FIH1Abstract Background/Aims: Emerging evidence shows that microRNAs (miRNAs) play a crucial role in the regulation of activation, proliferation and apoptosis of hepatic stellate cells (HSCs). Previous studies have indicated that miR-125a-5p is correlated with hepatitis B virus replication and disease progression. However, little is known about the biological role and underlying mechanism of miR-125a-5p in liver fibrosis. Methods: We analyzed the level of miR-125a-5p in carbon tetrachloride-induced liver fibrosis and activated HSCs. We analyzed the effects of miR-125a-5p down-regulation on HSC activation and proliferation. We also analyzed the binding of miR-125a-5p to the 3′-untranslated region of factor inhibiting hypoxia-inducible factor 1 (FIH1) mRNA. Results: Up-regulation of miR-125a-5p was observed in the liver tissues of fibrotic mice and activated HSCs. Down-regulation of miR-125a-5p prevented the activation and proliferation of HSCs. FIH1, a negative modulator of hypoxia inducible factor 1, was confirmed to be a target of miR-125a-5p using the luciferase reporter assay. Further studies demonstrated that miR-125a-5p prompted the activation and proliferation of HSCs, at least in part, by down-regulating FIH1. Conclusion: Our findings shed new light on miRNAs as a promising therapeutic target in liver fibrosis.G. Li and J. Li contributed equally to this work.

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Differential expression of microRNAs miR‐21, miR‐31, miR‐203, miR‐125a‐5p and miR‐125b and proteins PTEN and p63 in verrucous carcinoma of the head and neck

Cited by 41 publications

References 39 publications

Analysis of MicroRNAs in Sputum to Improve Computed Tomography for Lung Cancer Diagnosis

Analysis of MicroRNAs in Sputum to Improve Computed Tomography for Lung Cancer Diagnosis

MicroRNA expression differs in cutaneous squamous cell carcinomas and healthy skin of immunocompetent individuals

MicroRNA-125a-5p Contributes to Hepatic Stellate Cell Activation through Targeting FIH1

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