Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening

Ng, Enders K.W.; Chong, Wilson; Jin, Hongchuan; Lam, Emily Kai Yee; Shin, Vivian Y.; Yu, Jing; Poon, Terence C.W.; Ng, Simon Siu Man; Sung, Joseph J.Y.

doi:10.1136/gut.2008.167817

Cited by 1,040 publications

(911 citation statements)

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“…Specific serum miRNA expression profiles show a great potential to serve as a novel class of biomarkers for the diagnosis of cancer and other diseases [5,6]. Numerous reports have subsequently shown that unique expression patterns of circulating miRNAs reflect various physiological and pathological conditions [7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA

et al. 2011

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Our previous studies have demonstrated that stable microRNAs (miRNAs) in mammalian serum and plasma are actively secreted from tissues and cells and can serve as a novel class of biomarkers for diseases, and act as signaling molecules in intercellular communication. Here, we report the surprising finding that exogenous plant miRNAs are present in the sera and tissues of various animals and that these exogenous plant miRNAs are primarily acquired orally, through food intake. MIR168a is abundant in rice and is one of the most highly enriched exogenous plant miRNAs in the sera of Chinese subjects. Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 (LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. These findings demonstrate that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.

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Section: Introductionmentioning

confidence: 99%

Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA

et al. 2011

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“…RNU19 or RNU6B was used as controls for tissue and cell analysis (Chen and Stallings, 2007;Liu et al, 2010). miR-16 was used as a control for the plasma analysis (Ng et al, 2009). mRNA expression of TGFb-RII and glyceraldehyde 3-phosphate dehydrogenase (internal control) was also analyzed using TaqMan qPCR system (Applied Biosystems).…”

Section: Gc Tissues and Plasma Samplesmentioning

confidence: 99%

Overexpression of miR-370 and downregulation of its novel target TGFβ-RII contribute to the progression of gastric carcinoma

et al. 2011

Oncogene

101

100

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MicroRNAs (miRNAs) are endogenous non-coding RNAs that are known to be involved in the pathogenesis of tumors. Gastric carcinoma (GC) is a common malignancy worldwide. The aim of this study was the identification of the expression signature and functional roles of aberrant miRNAs in GC. Initial screening established a profile of aberrantly expressed miRNAs in tumors. miR-370 was confirmed to be overexpressed in GC tissues. Higher expression of miR-370 in GC tissues was associated with more advanced nodal metastasis and a higher clinical stage compared with controls. In addition, significantly higher level of miR-370 was noted in the plasma of GC patients compared with controls. Patients having more invasive or advanced tumors also exhibited a higher plasma level of miR-370. In vitro assays indicated that exogenous miR-370 expression enhanced the oncogenic potential of GC cells. The AGS-GFPM2 cells with exogenous miR-370 expression also exhibited enhanced abdominal metastatic dissemination in nude mice. Reporter assays confirmed that miR-370 targeted predicted sites in 3 0 UTR of transforming growth factor-b receptor II (TGFb-RII) gene. The exogenous miR-370 expression decreased TGFb-RII expression and the phosphorylation of Smad3 elicited by TGFb1. The TGFb1-mediated repression in cell migration was reverted by exogenous miR-370 expression. A reverse correlation between miR-370 and TGFb-RII expression was noted in GC tissues. This study concludes that miR-370 is a miRNA that is associated with GC progression by downregulating TGFb-RII. The miRNA expression profile described in this study should contribute to future studies on the role of miRNAs in GC.

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“…Because serum and plasma are easily accessible, circulating biomarkers are one of the most promising means of diagnosis. Related studies have shown that human serum contains miRNAs and the expression pattern of these serum miRNAs can potentially be used to identify various types of cancer (Chen et al, 2008;Gilad et al, 2008;Mitchell et al, 2008;Ng et al, 2009;Resnick et al, 2009). Given that endogenous miRNAs exist in a form that is resistant to RNase activity (Patrick et al, 2008), identifying a unique serum miRNA expression in esophageal cancer can potentially assist tumor diagnosis and cancer treatment.…”

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confidence: 99%

Serum miR-21 Expression in Human Esophageal Squamous Cell Carcinomas

Cai¹,

Gao²,

Wei³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Differential expression of microRNAs in plasma of patients with colorectal cancer: a potential marker for colorectal cancer screening

Abstract: MiR-92 is significantly elevated in plasma of patients with CRC and can be a potential non-invasive molecular marker for CRC screening.

Cited by 1,040 publications

References 29 publications

Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA

Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA

Overexpression of miR-370 and downregulation of its novel target TGFβ-RII contribute to the progression of gastric carcinoma

Serum miR-21 Expression in Human Esophageal Squamous Cell Carcinomas

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