Differential expression of miRNAs in a human developing neuronal cell line chronically infected with Zika virus

Abstract

Zika virus (ZIKV) is a serious public health concern that may lead to neurological disorders in affected individuals. The virus can be transmitted from an infected mother to her fetus, via mosquitoes, or sexually. ZIKV infections are associated with increased risk for Guillain-Barré syndrome (GBS) and congenital microcephaly in newborns infected prenatally. Dysregulations of intracellular microRNAs (miRNAs) in infected neurons have been linked to different neurological diseases. To determine the potential role of miRNAs in ZIKV infection we developed a chronically infected neuroblastoma cell line and carried out differential expression analyses of miRNAs with reference to an uninfected neuroblastoma cell line. A total of 3192miRNAs were evaluated and 389 were found to be upregulated < 2-fold and 1291 were downregulated < 2-fold. In particular, we determined that hsa-mir- 431-5p, hsa-mir-3687, hsamir-4655-5p, hsa-mir-6071, hsa-mir-762, hsa-mir-5787, and hsa-mir-6825-3p were significantly downregulated, ranging from -5711 to -660-fold whereas, has- mir-4315, hsa-mir-5681b, hsamir-6511a-3p, hsa-mir-1264, hsa-mir-4418, hsa-mir-4497, hsa-mir-4485-3p, hsa-mir-4715-3p, hsamir-4433-3p, hsa-mir-4708-3p, hsa-mir-1973 and hsa-mir-564 were upregulated, ranging from 20-0.8-fold. We carried out target gene alignment of these miRNAs with the ZIKV genome to predict the function of the differentially expressed miRNAs and their potential impact on ZIKV pathogenesis. These miRNAs might prove useful as novel diagnostic or therapeutic markers and targets for further research on ZIKV infection and neuronal injury resulting from ZIKV infectivity in developing fetal brain neurons.

The Correlation Between Peripheral Blood MicroRibonucleic Acid Expression Level and Personality Disorder in Patients with Schizophrenia

“… 36 MiR-3687, miR-4725-3P, and miRNA-5096 are involved in pathways that regulate glial cell tumorigenesis in the nervous system. 37 - 39 The above studies can partly explain why the miRNA in this study has abnormal expression in schizophrenia, because the above processes may lead to the occurrence and development of schizophrenia. Previous studies have also shown that miRNAs associated with schizophrenia have something in common and are related to the exposure and response of stressor, 40 and the correlation between the activation level of stress response and schizophrenia has also been reported, which seems to explain the differential expression of some mRNAs in schizophrenia.…”

Biomarker profiling to determine clinical impact of microRNAs in cognitive disorders

“…We also identified several novel DE-miRNAs, including hsa-miR-548as-5p, hsa-miR-519c-3p, hsa-miR-6883-5p, hsa-miR-5186, hsa-miR-548ak, and hsa-miR-147a. The remaining DE-miRNAs have been implicated in infectious diseases 51 , endocrine diseases 52 and neoplastic diseases 53 – 56 , but not in CNS diseases or any cognitive disorders. Notably, changes in cellular developmental pathways do not yield the same outcome in every disease; in cancers, they cause rapid cell growth and proliferation, whereas in CNS disorders, they accelerate neuron aging and death.…”

Mapping CircRNA–miRNA–mRNA regulatory axis identifies hsa_circ_0080942 and hsa_circ_0080135 as a potential theranostic agents for SARS-CoV-2 infection

“…The work performed by Sung et al, revealed anti-HIV-1 activity of miR-198 via targeting cyclin T1 [ 79 ]. Bagasra et al, found down-regulation of has-miR-5787 in Zika virus infected neuronal cell line [ 80 ]. A number of other studies have revealed down-regulation of miRNA targets of prioritized circRNAs in viral infections and other diseases.…”